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Antibacterial Performance of a Streptomyces spectabilis Strain Producing Metacycloprodigiosin

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Abstract

After separation of bacterial colonies on solid plates, purification, and screening through the agar cup-plate method, an antibiotic-resistant bacterial isolate was obtained, and named strain L20190601, the 16S rRNA gene sequence data of strain L20190601 to GenBank, NCBI have provided GenBank accession number MW931615. 16S rRNA gene sequencing revealed that this isolate was highly similar to a number of Streptomyces species. Among them, the homology with S. spectabilis was the highest, reaching 99.9, together with curved hyphal morphology and biochemical tests, allowed us to identify strain L20190601 as S. spectabilis. The red pigment produced by S. spectabilis strain L20190601 was structurally identified. An acid–base color reaction assay showed that when this pigment was dissolved in a solution at pH 3.0 and 9.0, the color of the solution was red and yellow, respectively. In addition, the analysis of absorption spectra revealed that at pH 8.0 and 3.0, the maximum absorption peaks were at 466 and 531 nm, respectively. These results are consistent with the spectral absorption characteristics of metacycloprodigiosin reported in the literature. Moreover, the retention time of purified pigments was identical to those of standard metacycloprodigiosin solutions. Mass spectrometry analysis revealed that the molecular weight of the red compound was 392.2 [M + H]+. Finally, metacycloprodigiosin was found to be effective against eight clinically common pathogens: Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Streptococcus pyogenes, Pseudomonas aeruginosa, Bacillus typhi, Candida albicans, and Trichophyton rubrum. In summary, metacycloprodigiosin exhibited strong antibacterial activity and a broad antibacterial spectrum, and thus is a promising compound for the development of a new type of antibacterial drug.

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Acknowledgements

This research was supported by the Natural Science Foundation of Guangxi Natural Science Foundation (Grant Number, 2018GXNSFBA294004), the Natural Science Foundation of Hubei Province (Grant Number, 2020CFB206), the Hubei Provincial Department of Education (Grant Number, Q20202805), the Guangxi Autonomous Region University Student Innovation and Entrepreneurship Project (Grant Numbers 202010605054 and 202010605068), and the Doctoral Foundation of HuBei University of Science & Technology Science (Grant Number BK202028).

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Authors and Affiliations

  1. College of Clinical Medicine, HuBei University of Science and Technology, Xianning, 437000, Hubei, China

    L. I. Meng-xi

  2. Medicine Research Institute, HuBei University of Science and Technology, Xianning, 437000, China

    Zhang Zhen-wang

  3. College of Chemical and Biological Engineering, Hechi University, Hechi, 546300, China

    L. I. Meng-xi, Huang Hui-bin, Long Jie-yun & C. A. O. Jing-xiao

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  1. L. I. Meng-xi
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  2. Huang Hui-bin
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  3. Long Jie-yun
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Correspondence to Zhang Zhen-wang.

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Meng-xi, L.I., Hui-bin, H., Jie-yun, L. et al. Antibacterial Performance of a Streptomyces spectabilis Strain Producing Metacycloprodigiosin. Curr Microbiol 78, 2569–2576 (2021). https://doi.org/10.1007/s00284-021-02513-w

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