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Orange Bagasse Pellets as a Carbon Source for Biobutanol Production

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Abstract

Due to the environmental concerns, the conversion of lignocellulosic waste can be the key to produce bioproducts and biofuels such as butanol. This study aimed to present and evaluate orange bagasse pellets (OBP) as a carbon source to produce butan-1-ol production via ABE fermentation using Clostridium beijerinckii. These bagasse pellets were characterized, holocellulose (18.99%), alfacellulose (5.37%), hemicellulose (13.62%), lignin (6.16%), pectin (7.21%), protein (3.14%), and was tested under three different pretreatments, which were the following: (a) ultrasound, (b) autohydrolysis, and (c) acid-diluted hydrolysis followed by enzymatic hydrolysis to verify an amount of fermentable total reducing sugars. ANOVA was used and pretreatments followed by enzymatic hydrolysis do not enhance a significant amount of available sugars compared to raw bagasse. The ABE fermentation was carried out in batch reactors at 37 °C under agitation of 160 rpm and anaerobic conditions, using OBP without treatment followed by enzymatic hydrolysis. Using a non-mutant microorganism, the fermentation achieved butyric acid yields of 3762.68 mg L−1 for control and 2488.82 mg L−1 for OBP and the butanol production was 63.86 mg L−1 and 196.80 mg L−1 for OBP and the control (glucose) assay, respectively. The results of this solvent’s production have shown that OBP has the potential for ABE fermentation and a promising feedstock.

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Acknowledgements

The authors would like to thank Biomass and the Bioenergy Laboratory from UFSCar-Brazil for their assistance and providing equipments. The present work had no financial support by any funding organization.

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Authors and Affiliations

  1. Department of Biology, Federal University of São Carlos-UFSCar, Campus Sorocaba, Rodovia João Leme dos Santos km 110 SP-264, Bairro Itinga, Sorocaba, SP, 18052-780, Brazil

    Gabriela Fiori da Silva, Tiago Palladino Delforno & Iolanda Cristina Silveira Duarte

  2. Department of Physics, Chemistry and Mathematics, Federal University of São Carlos-UFSCar, Campus Sorocaba, Sorocaba, SP, 18052-780, Brazil

    Samir Leite Mathias & Aparecido Junior de Menezes

  3. Faculty of Engineering of Sorocaba-FACENS, Sorocaba, SP, Brazil

    João Guilherme Pereira Vicente

  4. Department of Hydraulics and Sanitation, School of Engineering of São Carlos, University of São Paulo-USP, São Paulo, SP, Brazil

    Maria Bernadete Amâncio Varesche

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  1. Gabriela Fiori da Silva
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  2. Samir Leite Mathias
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Contributions

GFS and ICSD conceived the present idea. GFS carried out the experiments and wrote the manuscript with support from ICSD that supervised the project. JGPV, SLM, AJM, and MBAV contributed to analytical methods. GFS and TPD contributed to the final version of the manuscript. All authors made substantial contributions to concept this research.

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Correspondence to Gabriela Fiori da Silva.

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da Silva, G.F., Mathias, S.L., de Menezes, A.J. et al. Orange Bagasse Pellets as a Carbon Source for Biobutanol Production. Curr Microbiol 77, 4053–4062 (2020). https://doi.org/10.1007/s00284-020-02245-3

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