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Survival, Intestinal Mucosa Adhesion, and Immunomodulatory Potential of Lactobacillus plantarum Strains

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Abstract

Survival during transit through the gastrointestinal track, intestinal mucosa adhesion, and a potential immunomodulatory effect of Lactobacillus plantarum strains 2035 and ACA-DC 2640 were investigated in a rat model. According to microbiological and multiplex PCR analysis, both strains were detected in feces 24 h after either single-dose or daily administration for 7 days. Intestinal mucosa adhesion of L. plantarum 2035 was noted in the large intestine at 24 h after single-dose administration, while it was not detected at 48 h. Daily dosing, prolonged detection of the strain up to 48 h post-administration, and expanded adhesion to the small intestine. Adhesion of L. plantarum ACA-DC 2640 to the intestinal mucosa after single-dose administration was prolonged and more extended compared to L. plantarum 2035. Daily dosing increased both the levels and the rate of positive cultures of the strains compared to those of the single-dose scheme. In addition, both strains increased total IgG while decreased IgM and IgA serum levels. In conclusion, L. plantarum 2035 and L. plantarum ACA-DC 2640 survived transit through the gastrointestinal track, exhibited transient distinct adhesion to the intestinal mucosa and modulated the systemic immune response.

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Acknowledgements

The authors wish to thank Assistant Professor A. Galanis for his valuable technical support and scientific advice on molecular analysis.

Funding

The present research project was co-financed by the European Union (European Regional Development Fund–ERDF) and Greek national funds through the Operational Program “National Action “COOPERATION 2011—Partnerships of Production and Research Institutions in Focused Research and Technology Sectors” (Project Nr. 11SYN_2_571- ProbioDairyMeat).

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Authors and Affiliations

  1. Laboratory of Applied Microbiology & Biotechnology, Department of Molecular Biology & Genetics, Democritus University of Thrace, 68100, Alexandroupolis, Greece

    Valentini Santarmaki, Yiannis Kourkoutas & Mikis Kiourtzidis

  2. Laboratory of Dairy Research, Department of Food Science and Human Nutrition, Agricultural University of Athens, 11855, Athens, Greece

    Georgia Zoumpopoulou & Effie Tsakalidou

  3. Laboratory of Cell Proliferation and Ageing, Institute of Biosciences and Applications, National Centre for Scientific Research Demokritos, 15310, Athens, Greece

    Eleni Mavrogonatou

  4. Institute of Technology of Agricultural Products, Hellenic Agricultural Organization DEMETER, 1 S. Venizelou Str, Lykovrissi, 14123, Athens, Greece

    Nikos Chorianopoulos & Chrysoula Tassou

  5. Laboratory of Experimental Surgery and Surgical Research, School of Medicine, Democritus University of Thrace, 68100, Alexandroupolis, Greece

    Constantinos Simopoulos & Petros Ypsilantis

  6. University General Hospital of Alexandroupolis, Dragana, 68100, Alexandroupolis, Greece

    Petros Ypsilantis

Authors
  1. Valentini Santarmaki
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Correspondence to Petros Ypsilantis.

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The authors declare no conflict of interest.

Ethical Approval

The experimental protocol was approved by the Animal Care and Use Committee of the local Veterinary Service since it was in accordance with the requirements set by PD 160/91, which complied with Directive 2010/63/EU, which was the legislation in force at the time of experimentation.

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Santarmaki, V., Kourkoutas, Y., Zoumpopoulou, G. et al. Survival, Intestinal Mucosa Adhesion, and Immunomodulatory Potential of Lactobacillus plantarum Strains. Curr Microbiol 74, 1061–1067 (2017). https://doi.org/10.1007/s00284-017-1285-z

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  • DOI: https://doi.org/10.1007/s00284-017-1285-z

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