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Characterization of Non-coding Regions in B Mating Loci of Agrocybe salicacola Groups: Target Sites for B Mating Type Identification

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Abstract

Agrocybe salicacola is a delicious and cultivable mushroom. It is important to understand this species’ inherent characteristics, especially to elucidate the constitution and segregation of mating genes. In this study, two compatible B mating loci in strain YAASM0711 of A. salicacola were cloned from the monokaryons, and sequence and phylogeny analyses showed two conserved genes encoding pheromone receptors maybe lost mating activity, which determined by comparing with those of other mushrooms. In the conserved regions of mating loci, partial insertion/deletion fragments made non-coding regions posses polymorphisms, and monokaryotic strains of different mating types were distinguished from each other according to the amplification profile of variable regions, which suggested mating loci were integrally assigned to offspring strains during mitosis in A. salicacola. As our known, it is the first to develop molecular markers for B mating-type identification using variable non-coding fragments of mating loci in basidiomycetes.

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References

  1. Badrane H, May G (1999) The divergence-homogenization duality in the evolution of the b1 mating type gene of Coprinus cinereus. Mol Biol Evol 16:975–986

    Article  CAS  PubMed  Google Scholar 

  2. Bao D, Gong M, Zheng H, Chen M, Zhang L, Wang H, Jiang J, Wu L, Zhu Y, Zhu G, Zhou Y, Li C, Wang S, Zhao Y, Zhao G, Tan Q (2013) Sequencing and comparative analysis of the straw mushroom (Volvariella volvacea) genome. PLoS ONE 8:e58294

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Brewer MT, Cadle-Davidson L, Cortesi P, Spanu PD, Milgroom MG (2011) Identification and structure of the mating-type locus and development of PCR-based markers for mating type in powdery mildew fungi. Fungal Genet Biol 48:704–713

    Article  CAS  PubMed  Google Scholar 

  4. Chen WM, Chai HM, Zhou HM, Li SH, Zhao YC (2012) Phylogenetic analysis of the Agrocybe aegerita multispecies complex in Southwest China inferred from ITS and mtSSU rDNA sequences and mating tests. Ann Microbiol 4:1791–1801

    Article  Google Scholar 

  5. Debuchy R, Berteaux-Lecellier V, Silar P (2010) Mating systems and sexual morphogenesis in ascomycetes. Cellular and molecular biology of filamentous fungi. ASM Press, Washington, DC, pp 501–535

    Chapter  Google Scholar 

  6. Duong TA, De Beer ZW, Wingfield BD, Wingfield MJ (2013) Characterization of the mating-type genes in Leptographium procerum and Leptographium profanum. Fungal Biol 117:411–421

    Article  CAS  PubMed  Google Scholar 

  7. Duong TA, De Beer ZW, Wingfield BD, Wingfield MJ (2016) Mating type markers reveal high levels of heterothallism in Leptographium sensu lato. Fungal Biol 120:538–546

    Article  CAS  PubMed  Google Scholar 

  8. Groenewald M, Groenewald JZ, Harrington TC, Abeln ECA, Crous PW (2006) Mating type gene analysis in apparently asexual Cercospora species is suggestive of cryptic sex. Fungal Genet Biol 43:813–825

    Article  CAS  PubMed  Google Scholar 

  9. Halsall JR, Milner MJ, Casselton LA (1999) Three subfamilies of pheromone and receptor genes generate multiple B mating specificities in the mushroom Coprinopsis cinerea. Genetics 154:1115–1123

    Google Scholar 

  10. James TY, Liou SR, Vilgalys R (2004) The genetic structure and diversity of the A and B mating-type genes from the tropical oyster mushroom, Pleurotus djamor. Fungal Genet Biol 41:813–825

    Article  CAS  PubMed  Google Scholar 

  11. Ni M, Feretzaki M, Sun S, Wang XY, Heitman J (2011) Sex in fungi. Annu Rev Genet 45:405–430

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Niculita-Hirzel H, Labbé J, Kohler A, Le Tacon F, Martin F, Sanders IR, Kües U (2008) Gene organization of the mating type regions in the ectomycorrhizal fungus Laccaria bicolor reveals distinct evolution between the two mating type loci. N Phytol 180:329–342

    Article  CAS  Google Scholar 

  13. Ohm RA, de Jong JF, Lugones LG, Aerts A, Kothe E, Stajich JE, de Vries RP, Record E, Levasseur A, Baker SE, Bartholomew KA, Coutinho PM, Erdmann S, Fowler TJ, Gathman AC, Lombard V, Henrissat B, Knabe N, Kües U, Lilly WW, Lindquist E, Lucas S, Magnuson JK, Piumi F, Raudaskoski M, Salamov A, Schmutz J, Schwarze FW, van Kuyk PA, Horton JS, Grigoriev IV, Wösten HA (2010) Genome sequence of the model mushroom Schizophyllum commune. Nat Biotechnol 28:957–963

    Article  CAS  PubMed  Google Scholar 

  14. Paoletti M, Buck KW, Brasier CM (2005) Cloning and sequence analysis of the MAT-B (MAT-2) genes from the three Dutch elm disease pathogens, Ophiostoma ulmi, O. novo-ulmi and O. himalulmi. Mycol Res 109:983–991

    Article  CAS  PubMed  Google Scholar 

  15. Raudaskoski M, Kothe E (2010) Basidiomycete mating type genes and pheromone signaling. Eukaryot Cell 9:847–859

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Riquelme M, Challen MP, Casselton LA, Brown AJ (2005) The origin of multiple B mating specificities in Coprinopsis cinerea. Genetics 170:1105–1119

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Ryu JS, Kim MK, Ro HS, Kang YM, Kwon JH, Kong WS, Lee HS (2012) Identification of mating type loci and development of SCAR marker genetically linked to the B3 locus in Pleurotus eryngii. J Microbiol Biotechnol 22:1177–1184

    Article  CAS  PubMed  Google Scholar 

  18. Santos JM, Correia VG, Phillips AJL (2010) Primers for mating-type diagnosis in Diaporthe and Phomopsis: their use in teleomorph induction in vitro and biological species definition. Fungal Biol 114:255–270

    Article  CAS  PubMed  Google Scholar 

  19. Swofford DL (2002) PAUP*. Phylogenetic analysis using parsimony (*and other methods). Sinauer Associates Handbook and Software, Sunderland

  20. Untergasser A, Cutcutache I, Koressaar T, Ye J, Faircloth BC, Remm M, Rozen SG (2012) Primer3—new capabilities and interfaces. Nucleic Acids Res 40:e115

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Vaillancourt LJ, Raudaskoski M, Specht CA, Raper CA (1997) Multiple genes encoding pheromones and a pheromone receptor define the B beta 1 mating-type specificity in Schizophyllum commune. Genetics 146:541–551

    CAS  PubMed  PubMed Central  Google Scholar 

  22. Van Peer AF, Park SY, Shin PG, Jang KY, Yoo YB, Park YJ, Lee BM, Sung GH, James TY, Kong WS (2011) Comparative genomics of the mating-type loci of the mushroom Flammulina velutipes reveals widespread synteny and recent inversions. PLoS ONE 6:e22249

    Article  PubMed  PubMed Central  Google Scholar 

  23. Wada R, J-i Maruyama, Yamaguchi H, Yamamoto N, Wagu Y, Paoletti M, Archer DB, Dyer PS, Kitamoto K (2012) Presence and functionality of mating type genes in the supposedly asexual filamentous fungus Aspergillus oryzae. Appl Environ Microbiol 78:2819–2829

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Wu L, Van Peer A, Song WH, Wang H, Chen MJ, Tan Q, Song CY, Zhang MY, Bao DP (2013) Cloning of the Lentinula edodes B mating-type locus and identification of the genetic structure controlling B mating. Gene 531:270–278

    Article  CAS  PubMed  Google Scholar 

  25. Yang ZL, Zang M, Liu XX (1993) Agrocybe salicacola, a new species of sect. Aporus from Yunnan. Acta Bot Yunnanica 15:18–20

    Google Scholar 

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Acknowledgements

This work was supported by the National Natural Science Foundation Program of PR China (31360024, 31460014) and the China Agriculture Research System (CARS-24).

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Authors and Affiliations

  1. Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650223, Yunnan, China

    Weimin Chen, Hongmei Chai, Weixian Yang, Xiaolei Zhang & YongChang Zhao

  2. Yunnan Provincial Key Lab of Agricultural Biotechnology, Kunming, 650223, Yunnan, China

    Weimin Chen, Hongmei Chai, Xiaolei Zhang & YongChang Zhao

  3. Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, Kunming, 650223, Yunnan, China

    Weimin Chen, Hongmei Chai, Xiaolei Zhang & YongChang Zhao

  4. College of Life Science, Southwest Forestry University, Kunming, 650224, Yunnan, China

    Weixian Yang & Yuhui Chen

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  1. Weimin Chen
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  2. Hongmei Chai
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  3. Weixian Yang
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Correspondence to YongChang Zhao.

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Chen, W., Chai, H., Yang, W. et al. Characterization of Non-coding Regions in B Mating Loci of Agrocybe salicacola Groups: Target Sites for B Mating Type Identification. Curr Microbiol 74, 772–778 (2017). https://doi.org/10.1007/s00284-017-1247-5

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  • DOI: https://doi.org/10.1007/s00284-017-1247-5

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