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Response Mechanisms in Serratia marcescens IBBPo15 During Organic Solvents Exposure

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Abstract

Serratia marcescens strain IBBPo15 (KT315653) which possesses serratiopeptidase (ser) gene (KT894207) exhibited good solvent tolerance. During the exposure of S. marcescens IBBPo15 cells to 5 % organic solvents, n-decane was less toxic for this bacterium, compared with n-hexane, cyclohexane, ethylbenzene, toluene, and styrene. The exposure of the S. marcescens IBBPo15 cells to n-hexane, cyclohexane, ethylbenzene, toluene, and styrene induced the formation of large clusters, while in control and n-decane-exposed cells, only organization into small clusters was observed. The data obtained suggested that S. marcescens IBBPo15 cells produced some secondary metabolites (i.e., surfactant serrawettin, red pigment prodigiosin) which are well known as valuable molecules due to their large applications. The exposure of the bacterial cells to organic solvents induced secondary metabolites profile modifications. However, S. marcescens IBBPo15 possesses only alkB1, todM, rhlAB, pswP, mpr, and ser genes, the unspecific amplification of other fragments being acquired also when the primers for alkM1, xylM, ndoM, and C23DO genes were used. Modifications of DNA patterns were not depicted in S. marcescens IBBPo15 cells exposed to organic solvents.

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Acknowledgments

The study was funded by the Project No. RO1567-IBB05/2015 from the Institute of Biology Bucharest of Romanian Academy. The author is grateful to Ana Dinu, Alexandru Brînzan, and Gabriel Mihai Maria for technical support.

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  1. Institute of Biology Bucharest of Romanian Academy, 296 Splaiul Independentei, P.O. Box 56-53, 060031, Bucharest, Romania

    Mihaela Marilena Stancu

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Stancu, M.M. Response Mechanisms in Serratia marcescens IBBPo15 During Organic Solvents Exposure. Curr Microbiol 73, 755–765 (2016). https://doi.org/10.1007/s00284-016-1108-7

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  • DOI: https://doi.org/10.1007/s00284-016-1108-7

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