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Application of Differential Proteomic Analysis to Authenticate Ophiocordyceps sinensis

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Abstract

Ophiocordyceps sinensis (Berk.) Sacc. is one of the most well-known fungi in traditional Chinese medicine and is attracting attention because of its nutritious and medicinal properties. The present study aimed to produce a proteomic map to identify common O. sinensis proteins. The caterpillar body and stroma of O. sinensis collected from five locations and four fungal specimens of similar appearance were examined by two-dimensional electrophoresis (2-DE). Five proteins were identified using MALDI-TOF-–TOF/MS, and the 2-DE identification pattern was provided. OCS_04585 and β-lactamase domain-containing protein, the two abundant and characteristic proteins, were separated and purified using liquid-phase isoelectric focusing. The products were high-quality materials that can be used for future protein-function studies and immunoassay development.

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Authors and Affiliations

  1. Shenzhen Academy of Metrology and Quality Inspection, Shenzhen, 518102, People’s Republic of China

    Shiwei Zhang, Xintian Lai, Bifang Li, Cong Wu, Shifeng Wang, Xuejian Chen, Jingmin Huang & Guowu Yang

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  1. Shiwei Zhang
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  2. Xintian Lai
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  3. Bifang Li
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  4. Cong Wu
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  5. Shifeng Wang
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  6. Xuejian Chen
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  7. Jingmin Huang
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  8. Guowu Yang
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Correspondence to Xintian Lai.

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Zhang, S., Lai, X., Li, B. et al. Application of Differential Proteomic Analysis to Authenticate Ophiocordyceps sinensis . Curr Microbiol 72, 337–343 (2016). https://doi.org/10.1007/s00284-015-0950-3

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  • DOI: https://doi.org/10.1007/s00284-015-0950-3

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