Abstract
Indian pennywort (Centella asiatica (L.) Urban) is an important herbal plant with valuable medicinal properties. Irrigation with optimum freshwater is a basic crop requirement for plant growth, development, and secondary metabolite enrichment. The objective of the study was to evaluate the effect of different irrigation regimes on plant morphological, physiological, and biochemical responses as well as total centelloside content. Leaf greenness (SPAD), maximum quantum yield of PSII (Fv/Fm), and photon yield of PSII (ΦPSII) in plants grown under 64% field capacity (FC) were significantly reduced by 31.60%, 7.65%, and 25.91%, respectively, compared with plants grown under well irrigation (control; 100% FC). Remarkably, net photosynthetic rate (Pn), stomatal conductance (gs), and transpiration rate (Tr) in plants grown under 64% FC were more sensitive to water shortage, leading to a decline by 68.76%, 87.50%, and 84.08%, respectively, over the control. Leaf temperature (Tleaf) and crop water stress index (CWSI) under limited irrigation conditions (64% FC) were, respectively, increased by + 3.97 °C and 0.72 over the control in relation to decreased Tr (0.43 mmol H2O m−2 s−1) and altered stomatal functions indicated by low gs (0.02 mmol H2O m−2 s−1). Enhancement of glucose (2.70-fold over the control), fructose (2.14-fold over the control), and total soluble sugar (2.52-fold over the control) played a key role in osmotic adjustment when plants were exposed to moderate irrigation conditions (75% FC). Asiaticoside, asiatic acid, and total centellosides in the leaf tissues were enriched under limited irrigation conditions than those in the control, leading to the maximum centellosides yield at 75% FC. Number of green leaves, leaf area, stolon length, and shoot fresh weight in moderately irrigated plants (75% FC) were increased by 1.23, 1.24, 1.13, and 1.15-folds, respectively, over the control. The basic information obtained from this investigation provides a better understanding of the response of Indian pennywort under limited irrigation schedule and suggests an alternative way to manipulate biomass production and yield of total centellosides using moderate level of irrigation (75% FC).
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Afshar RK, Chaichi MR, Assareh MH, Hashemi M, Liaghat A (2014) Interactive effect of deficit irrigation and soil organic amendments on seed yield and flavonolignan production of milk thistle (Silybum marianum L. Gaertn.). Ind Crops Prod 58:166–172. https://doi.org/10.1016/j.indcrop.2014.03.043
Alinian S, Razmjoo J, Zeinali H (2016) Flavonoids, anthocynins, phenolics and essential oil produced in cumin (Cuminum cyminum L.) accessions under different irrigation regimes. Ind Crops Prod 81:49–55. https://doi.org/10.1016/j.indcrop.2015.11.040
Al-Jawad JY, Alsaffar HM, Bertram D, Kalin RM (2019) A comprehensive optimum integrated water resources management approach for multidisciplinary water resources management problems. J Environ Manage 239:211–224. https://doi.org/10.1016/j.jenvman.2019.03.045
An M, Fan L, Huang J, Yang W, Wu H, Wang X, Khanal R (2021) The gap of water supply—Demand and its driving factors: From water footprint view in Huaihe River Basin. PLoS ONE 16:e0247604. https://doi.org/10.1371/journal.pone.0247604
Askary M, Behdani MA, Parsa S, Mahmoodi S, Jamialahmadi M (2018) Water stress and manure application affect the quantity and quality of essential oil of Thymus daenensis and Thymus vulgaris. Ind Crops Prod 111:336–344. https://doi.org/10.1016/j.indcrop.2017.09.056
Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207. https://doi.org/10.1007/BF00018060
Bayat H, Moghadam AN (2019) Drought effects on growth, water status, proline content and antioxidant system in three Salvia nemorosa L. cultivars. Acta Physiol Plant 41:149. https://doi.org/10.1007/s11738-019-2942-6
Biju S, Fuentes S, Gupta D (2018) The use of infrared thermal imaging as a non-destructive screening tool for identifying drought-tolerant lentil genotypes. Plant Physiol Biochem 127:11–24. https://doi.org/10.1016/j.plaphy.2018.03.005
Carroll DA, Hansen NC, Hopkins BG, DeJonge KC (2017) Leaf temperature of maize and crop water stress index with variable irrigation and nitrogen supply. Irrig Sci 35:549–560. https://doi.org/10.1007/s00271-017-0558-4
Carvalho MHC (2008) Drought stress and reactive oxygen species: production, scavenging and signaling. Plant Signal Behav 3:156–165. https://doi.org/10.4161/psb.3.3.5536
Caser M, Chitarra W, D’Angiolillo F, Perrone I, Demasi S, Lovisolo C, Pistelli L, Pistelli L, Scariot V (2019) Drought stress adaptation modulates plant secondary metabolite production in Salvia dolomitica Codd. Ind Crops Prod 129:85–96. https://doi.org/10.1016/j.indcrop.2018.11.068
Caser M, D’Angiolillo F, Chitarra W, Lovisolo C, Ruffoni B, Pistelli L, Pistelli L, Scariot V (2018) Ecophysiological and phytochemical responses of Salvia sinaloensis Fern. to drought stress. Plant Growth Regul 84:383–394. https://doi.org/10.1007/s10725-017-0349-1
Cha-um S, Supaibulwatana K, Kirdmanee C (2006) Water relation, photosynthetic ability and growth of Thai jasmine rice (Oryza sativa L.ssp.indica cv. KDML 105) to salt stress by application of exogenous glycinebetaine and choline. J Agron Crop Sci 192:25–36. https://doi.org/10.1111/j.1439-037X.2006.00186.x
Cheng M, Jiao X, Guo W, Wang S, Pan Y, Zhang H, Sang H (2020) Temporal and spatial distribution characteristics of irrigation water requirement for main crops in the plain area of Hebei Province. Irrig Drain 69:1051–1062. https://doi.org/10.1002/ird.2489
Cozzolino R, Mari A, Ramezani S, Martignetti A, Piacente S, de Giulio B (2021) Assessment of volatile compounds as potential markers of water deficit stress of two wild ecotypes of Salvia reuterana Boiss. J Food Composit Anal 100:103939. https://doi.org/10.1016/j.jfca.2021.103939
de Miguel Á, Kallache M, García-Calvo E (2015) The water footprint of agriculture in Duero River Basin. Sustainability 7:6759–6780. https://doi.org/10.3390/su7066759
Devkota A, Jha PK (2011) Influence of water stress on growth and yield of Centella asiatica. Int Agrophys 25:211–214
Du Y, Zhao Q, Chen L, Yao X, Zhang W, Zhang B, Xie F (2020) Effect of drought stress on sugar metabolism in leaves and roots of soybean seedlings. Plant Physiol Biochem 146:1–12. https://doi.org/10.1016/j.plaphy.2019.11.003
Ekren S, Sönmez Ç, Özçakal E, Kurttaş YSK, Bayram E, Gürgülü H (2012) The effect of different irrigation water levels on yield and quality characteristics of purple basil (Ocimum basilicum L.). Agric Water Manage 109:155–161. https://doi.org/10.1016/j.agwat.2012.03.004
Ewaid SH, Abed SA, Al-Ansari N (2019) Crop water requirements and irrigation schedules for some major crops in Southern Iraq. Water 11:756. https://doi.org/10.3390/w11040756
Fu J, Huang B, Fry J (2010) Osmotic potential, sucrose level, and activity of sucrose metabolic enzymes in tall fescue in response to deficit irrigation. J Am Soc Hortic Sci 135:506−510. https://doi.org/10.21273/JASHS.135.6.506
Gagné-Bourque F, Bertrand A, Claessens A, Aliferis KA, Jabaji S (2016) Alleviation of drought stress and metabolic changes in timothy (Phleum pratense L.) colonized with Bacillus subtilis B26. Front Plant Sci 7:584. https://doi.org/10.3389/fpls.2016.00584
Gao S, Wang Y, Yu S, Huang Y, Liu H, Chen W, He X (2020) Effects of drought stress on growth, physiology and secondary metabolites of two Adonis species in Northeast China. Sci Hortic 259:108795. https://doi.org/10.1016/j.scienta.2019.108795
Gray NE, Magana AA, Lak P, Wright KM, Quinn J, Stevens JF, Maier CS, Soumyanath A (2018) Centella asiatica: Phytochemistry and mechanisms of neuroprotection and cognitive enhancement. Phytochem Rev 17:161–194. https://doi.org/10.1007/s11101-017-9528-y
Hossain I, Imteaz MA, Khastagir A (2021) Water footprint: Applying the water footprint assessment method to Australian agriculture. J Sci Food Agric 101:4090–4098. https://doi.org/10.1002/jsfa.11044
Hussain F, Bronson KF, Peng S (2000) Use of chlorophyll meter sufficiency indices for nitrogen management of irrigated rice in Asia. Agron J 92:875–879. https://doi.org/10.2134/agronj2000.925875x
Jat RS, Gajbhiye NA (2017) Secondary metabolites production influenced with soil fertility and irrigation in medicinal plant; Mandukaparni (Centella asiatica L.). Nat Acad Sci Lett 40:87–90. https://doi.org/10.1007/s40009-016-0531-4y
Jhansi D, Kola M (2019) The antioxidant potential of Centella asiatica: A review. J Med Plant Stud 7:18–20
Jiménez S, Dridi J, Gutiérrez D, Moret D, Irigoyen JJ, Moreno MA, Gogorcena Y (2013) Physiological, biochemical and molecular responses in four Prunus rootstocks submitted to drought stress. Tree Physiol 33:1061–1075. https://doi.org/10.1093/treephys/tpt074
Jisha S, Gouri PR, Anith KN, Sabu KK (2018) Piriformospora indica cell wall extract as the best elicitor for asiaticoside production in Centella asiatica (L.) Urban, evidenced by morphological, physiological and molecular analyses. Plant Physiol Biochem 125:106–115. https://doi.org/10.1016/j.plaphy.2018.01.021
Jones HG, Schofield P (2008) Thermal and other remote sensing of plant stress. Gen Appl Plant Physiol 34:19–32
Kalamartzis I, Dordas C, Georgiou P, Menexes G (2020) The use of appropriate cultivar of basil (Ocimum basilicum) can increase water use efficiency under water stress. Agronomy 10:70. https://doi.org/10.3390/agronomy10010070
Karkacier M, Erbas M, Uslu MK, Aksu M (2003) Comparison of different extraction and detection methods for sugars using amino-bonded phase HPLC. J Chromatog Sci 41:331–333. https://doi.org/10.1093/chromsci/41.6.331
Kirnak H, Irik HA, Unlukara A (2019) Potential use of crop water stress index (CWSI) in irrigation scheduling of drip-irrigated seed pumpkin plants with different irrigation levels. Sci Hortic 256:108608. https://doi.org/10.1016/j.scienta.2019.108608
Lanfermeijer FC, Koerselman-Kooij JW, Borstlap AC (1991) Osmosensitivity of sucrose uptake by immature pea cotyledons disappears during development. Plant Physiol 95:832–838. https://doi.org/10.1104/pp.95.3.832
Li P, Qian H (2018) Water resources research to support a sustainable China. Int J Water Resour Dev 34:327–336. https://doi.org/10.1080/07900627.2018.1452723
Li S, Liu J, Liu H, Qiu R, Gao Y, Duan A (2021) Role of hydraulic signal and ABA in decrease of leaf stomatal and mesophyll conductance in soil drought-stressed tomato. Front Plant Sci 12:711. https://doi.org/10.3389/fpls.2021.653186
Loggini B, Scartazza A, Brugnoli E, Navari-Izzo F (1999) Antioxidant defense system, pigment composition, and photosynthetic efficiency in two wheat cultivars subjected to drought. Plant Physiol 119:1091–1099. https://doi.org/10.1104/pp.119.3.1091
Maxwell K, Johnson GN (2000) Chlorophyll fluorescence—a practical guide. J Exp Bot 51:659–668. https://doi.org/10.1093/jexbot/51.345.659
Mekonnen MM, Hoekstra AY (2016) Four billion people facing severe water scarcity. Sci Adv 2:e1500323. https://doi.org/10.1126/sciadv.1500323
Müller V, Albert A, Winkler JB, Lankes C, Noga G, Hunsche M (2013a) Ecologically relevant UV-B dose combined with high PAR intensity distinctly affect plant growth and accumulation of secondary metabolites in leaves of Centella asiatica L. Urban J Photochem Photobiol b: Biol 127:161–169. https://doi.org/10.1016/j.jphotobiol.2013.08.014
Müller V, Lankes C, Zimmermann BF, Noga G, Hunsche M (2013b) Centelloside accumulation in leaves of Centella asiatica is determined by resource partitioning between primary and secondary metabolism while influenced by supply levels of either nitrogen, phosphorus or potassium. J Plant Physiol 170:1165–1175. https://doi.org/10.1016/j.jplph.2013.03.010
Mumivand H, Ebrahimi A, Morshedloo MR, Shayganfar A (2021) Water deficit stress changes in drug yield, antioxidant enzymes activity and essential oil quality and quantity of Tarragon (Artemisia dracunculus L.). Ind Crops Prod 164:113381. https://doi.org/10.1016/j.indcrop.2021.113381
Narayani M, Srivastava S (2017) Elicitation: a stimulation of stress in in vitro plant cell/tissue cultures for enhancement of secondary metabolite production. Phytochem Rev 16:1227–1252. https://doi.org/10.1007/s11101-017-9534-0
Nav SN, Ebrahimi SN, Sonboli A, Mirjalili MH (2021) Variability, association and path analysis of centellosides and agro-morphological characteristics in Iranian Centella asiatica (L.) Urban ecotypes. South Afr J Bot 139:254–266. https://doi.org/10.1016/j.sajb.2021.03.006
Nouri H, Stokvis B, Galindo A, Blatchford M, Hoekstra AY (2019) Water scarcity alleviation through water footprint reduction in agriculture: The effect of soil mulching and drip irrigation. Sci Total Environ 653:241–252. https://doi.org/10.1016/j.scitotenv.2018.10.311
Pereyra MS, Argüello JA, Bima PI (2021) Genotype-dependent architectural and physiological responses regulate the strategies of two oregano cultivars to water excess and deficiency regimes. Ind Crops Prod 161:113206. https://doi.org/10.1016/j.indcrop.2020.113206
Pipatsitee P, Theerawitaya C, Tiasarum R, Samphumphuang T, Singh HP, Datta A, Cha-um S (2022) Physio-morphological traits and osmoregulation strategies of hybrid maize (Zea mays) at the seedling stage in response to water-deficit stress. Protoplasma 259:869–883. https://doi.org/10.1007/s00709-021-01707-0
Prasad A, Mathur AK, Mathur A (2019) Advances and emerging research trends for modulation of centelloside biosynthesis in Centella asiatica (L.) urban-A review. Ind Crops Prod 141:111768. https://doi.org/10.1016/j.indcrop.2019.111768
Ramezani S, Abbasi A, Sobhanverdi S, Shojaeiyan A, Ahmadi N (2020) The effects of water deficit on the expression of monoterpene synthases and essential oils composition in Salvia ecotypes. Physiol Mol Biol Plants 26:2199–2207. https://doi.org/10.1007/s12298-020-00892-1
Rivas R, Falcão HM, Ribeiro RV, Machado EC, Pimentel C, Santos MG (2016) Drought tolerance in cowpea species is driven by less sensitivity of leaf gas exchange to water deficit and rapid recovery of photosynthesis after rehydration. South Afr J Bot 103:101–107. https://doi.org/10.1016/j.sajb.2015.08.008
Rossati A (2017) Global warming and its health impact. Int J Occup Environ Med 8:7. https://doi.org/10.15171/ijoem.2017.963
Ru C, Hu X, Wang W, Ran H, Song T, Guo Y (2020) Evaluation of the crop water stress index as an indicator for the diagnosis of grapevine water deficiency in greenhouses. Horticulturae 6:86. https://doi.org/10.3390/horticulturae6040086
Sabaragamuwa R, Perera CO, Fedrizzi B (2018) Centella asiatica (Gotu kola) as a neuroprotectant and its potential role in healthy ageing. Trends Food Sci Technol 79:88–97. https://doi.org/10.1016/j.tifs.2018.07.024
Salehi A, Tasdighi H, Gholamhoseini M (2016) Evaluation of proline, chlorophyll, soluble sugar content and uptake of nutrients in the German chamomile (Matricaria chamomilla L.) under drought stress and organic fertilizer treatments. Asian Pacific J Trop Biomed 6:886–891. https://doi.org/10.1016/j.apjtb.2016.08.009
Sánchez-Virosta Á, Sánchez-Gómez D (2020) Thermography as a tool to assess inter-cultivar variability in garlic performance along variations of soil water availability. Remote Sens 12:2990. https://doi.org/10.3390/rs12182990
Singh R, Gupta P, Khan F, Singh S, Sanchita Mishra T, Kumar A, Dhawan SS, Shirke PA (2018) Modulations in primary and secondary metabolic pathways and adjustment in physiological behaviour of Withania somnifera under drought stress. Plant Sci 272:42–54. https://doi.org/10.1016/j.plantsci.2018.03.029
Soni P, Abdin MZ (2017) Water deficit-induced oxidative stress affects artemisinin content and expression of proline metabolic genes in Artemisia annua L. FEBS Open Biol 7:367–381. https://doi.org/10.1002/2211-5463.12184
Souza JV, Ming LC, Santos MA, Simon JE, Juliani HR, Saad JC (2021) Effect of water regime and harvest stage on essential oil accumulation in basil plant growing in sandy soil. Irrig Sci 39:493–503. https://doi.org/10.1007/s00271-021-00719-1
Sreenivasulu N, Harshavardhan VT, Govind G, Seiler C, Kohli A (2012) Contrapuntal role of ABA: Does it mediate stress tolerance or plant growth retardation under long-term drought stress? Gene 506:265–273. https://doi.org/10.1016/j.gene.2012.06.076
Tardieu F, Granier C, Müller B (2011) Water deficit and growth. Co-ordinating processes without an orchestrator? Curr Opin Plant Biol 14:283–289. https://doi.org/10.1016/j.pbi.2011.02.002
Wei Q, Li Q, Jin Y, Li K, Lei N, Chen J (2019) Effects of clonal integration on photochemical activity and growth performance of stoloniferous herb Centella asiatica suffering from heterogeneous water availability. Flora 256:36–42. https://doi.org/10.1016/j.flora.2019.05.001
Wiriya-Alongkorn W, Spreer W, Ongprasert S, Spohrer K, Pankasemsuk T, Müller J (2013) Detecting drought stress in longan tree using thermal imaging. Maejo Int J Sci Technol 7:166–180
Wu HH, Zou YN, Rahman M, Ni QD, Wu QS (2017) Mycorrhizas alter sucrose and proline metabolism in trifoliate orange exposed to drought stress. Sci Rep 7:42389. https://doi.org/10.1038/srep42389
Wulandari S, Widyastuti Y, Yunus A (2020) Growth and yield responses of three accessions of Centella asiatica grown in lowland under varied watering intensities. IOP Conf Ser: Earth Environ Sci 466:012011. https://doi.org/10.1088/1755-1315/466/1/012011
Xiong D, Nadal M (2020) Linking water relations and hydraulics with photosynthesis. Plant J 101:800–815. https://doi.org/10.1111/tpj.14595
Yang D, Yang Y, Xia J (2021) Hydrological cycle and water resources in a changing world: A review. Geograp Sust 2:115–122. https://doi.org/10.1016/j.geosus.2021.05.003
Yasheshwar US, Sharma MP, Khan W, Ahmad S (2017) Variation in ornamental traits, physiological responses of Tagetes erecta L. and T. patula L. in relation to antioxidant and metabolic profile under deficit irrigation strategies. Sci Hortic 214:200–208. https://doi.org/10.1016/j.scienta.2016.11.037
Yousaf S, Hanif MA, Rehman R, Azeem MW, Racoti A (2020) Indian Pennywort. In Medicinal Plants of South Asia, pp 423‒437. Elsevier. https://doi.org/10.1016/B978-0-08-102659-5.00032-X
Zakar MZ, Zakar DR, Fischer F (2020) Climate change-induced water scarcity: a threat to human health. South Asian Stud 27:293–312
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The authors thank the National Science and Technology Development Agency (NSTDA) as the funding source (Grant Number P-20-52585).
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National Science and Technology Development Agency, P-20-52585, Suriyan Cha-um.
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SC-U: project leader, experimental layout, and manuscript preparation, HPS: data analysis and manuscript editing, PP and PP: plant material preparation, data collection and analysis, KT, and RT: establishment and execution of experiment, and physiological data collection and analysis, CT: measurement of morphological, centellosides assay, and overall growth characteristics.
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Theerawitaya, C., Pipatsitee, P., Taota, K. et al. Impact of irrigation regime on morpho-physiological and biochemical attributes and centelloside content in Indian pennywort (Centella asiatica). Irrig Sci 41, 23–34 (2023). https://doi.org/10.1007/s00271-022-00832-9
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DOI: https://doi.org/10.1007/s00271-022-00832-9