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Lipid Emulsion Enriched in Omega-3 PUFA Accelerates Wound Healing: A Placebo-Controlled Animal Study

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Abstract

Background

The Omega-3 (ω-3) polyunsaturated fatty acids (PUFAs) generate bioactive lipid mediators that reduce inflammation. The present study evaluated the effect of SMOFlipid containing ω-3 PUFAs on wound healing.

Methods

Rats were divided into a SMOFlipid (SMOF) group and a 0.9% saline (placebo) group, with eight rats in each group. Wound excision was performed on the dorsal surface of each rat. In the SMOF group, 1 gm/kg SMOFlipid was dissolved in 3 mL saline as a treatment; in the placebo group, 3 mL saline was prepared as a treatment. The treatments were administered intravenously at an initial rate of 0.2 mL/kg body weight/h immediately after wounding, for 72 h. Blood samples were collected for white blood cell, tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-10 measurements at the baseline and at 1, 6, 12, 24, 48, and 72 h after intervention. Wound areas were measured over a 2-week period after excision, and a histological examination was performed.

Results

Compared with the placebo group, SMOFlipid supplementation engendered significant decreases in the wound area on day 3 (78.28 ± 5.25 vs. 105.86 ± 8.89%), day 5 (72.20 ± 4.31 vs. 96.39 ± 4.72%), day 10 (20.78 ± 1.28 vs. 39.80 ± 10.38%), and day 14 (7.56 ± 0.61 vs. 15.10 ± 2.42%). The placebo group had a higher TNF-α level than the SMOF group at 72 h. The IL-10 level was higher in the SMOF group than in the placebo group at 48 h. Histological analysis revealed a higher rate of fibroblast distribution and collagen fiber organization in the SMOF group (P = 0.01).

Conclusion

SMOFlipid enriched in ω-3 PUFA accelerates wound healing.

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Acknowledgements

This work was partially supported by the Tzu Chi University (TCIRP 101002-01Y1). This manuscript was edited by Wallace Academic Editing.

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Author notes

  1. Yi-Chi Peng and Fwu-Lin Yang have contributed equally to this work.

Authors and Affiliations

  1. Department of Nursing, Asia University, Taichung, Taiwan

    Yi-Chi Peng

  2. Intensive Care Unit, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, New Taipei City, Taiwan

    Fwu-Lin Yang

  3. School of Medicine, Tzu Chi University, Hualien, Taiwan

    Fwu-Lin Yang

  4. Department of Nursing, National Taichung University of Science and Technology, Taichung, Taiwan

    Yi-Maun Subeq

  5. Department of Nursing, Tzu Chi University of Science and Technology, Hualien, Taiwan

    Chin-Chieh Tien

  6. Department of Nursing, Mackay Medical College, New Taipei City, Taiwan

    Yann-Fen C. Chao

  7. Institute of Medical Sciences, Tzu Chi University, No. 701, Sec. 3., Zhongyang Rd., 97004, Hualien, Taiwan

    Ru-Ping Lee

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  1. Yi-Chi Peng
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Correspondence to Ru-Ping Lee.

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Peng, YC., Yang, FL., Subeq, YM. et al. Lipid Emulsion Enriched in Omega-3 PUFA Accelerates Wound Healing: A Placebo-Controlled Animal Study. World J Surg 42, 1714–1720 (2018). https://doi.org/10.1007/s00268-017-4404-x

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  • DOI: https://doi.org/10.1007/s00268-017-4404-x

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