Abstract
Purpose
Repair of bone defects, particularly critical-sized bone defects, is a considerable challenge in orthopaedics. Tissue-engineered bones provide an effective approach. However, previous studies mainly focused on the repair of bone defects in small animals. For better clinical application, repairing critical-sized bone defects in large animals must be studied. This study investigated the effect of a tissue-engineered bone for repairing critical-sized bone defect in sheep.
Methods
A tissue-engineered bone was constructed by culturing bone marrow mesenchymal-stem-cell-derived osteoblast cells seeded in a porous β-tricalcium phosphate ceramic (β-TCP) scaffold in a perfusion bioreactor. A critical-sized bone defect in sheep was repaired with the tissue-engineered bone. At the eighth and 16th week after the implantation of the tissue-engineered bone, X-ray examination and histological analysis were performed to evaluate the defect. The bone defect with only the β-TCP scaffold served as the control.
Result
X-ray showed that the bone defect was successfully repaired 16 weeks after implantation of the tissue-engineered bone; histological sections showed that a sufficient volume of new bones formed in β-TCP 16 weeks after implantation. Eight and 16 weeks after implantation, the volume of new bones that formed in the tissue-engineered bone group was more than that in the β-TCP scaffold group (P < 0.05).
Conclusion
Tissue-engineered bone improved osteogenesis in vivo and enhanced the ability to repair critical-sized bone defects in large animals.
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Acknowledgments
The authors are grateful to Shandong Hongli Medical Animal Experimental Research Co Ltd for kindly supplying and breeding the sheep. This research was supported by the Natural Science Foundation of Shandong Province (ZR2010HQ033) and the Independent Innovation Foundation of Shandong University (IIFSDU) (2010TS032).
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Li, D., Li, M., Liu, P. et al. Tissue-engineered bone constructed in a bioreactor for repairing critical-sized bone defects in sheep. International Orthopaedics (SICOT) 38, 2399–2406 (2014). https://doi.org/10.1007/s00264-014-2389-8
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DOI: https://doi.org/10.1007/s00264-014-2389-8