Abstract
Menaquinone-7 is a form of vitamin K2 that has been shown to have numerous healthy benefits. In this study, several surfactants were investigated to enhance the production of menaquinone-7 in Bacillus natto. Results showed that Brij-58 supplementation influenced the cell membrane via adsorption, and changed the interfacial tension of fermentation broth, while the changes in the state and the composition of the cell membrane enhanced the secretion and biosynthesis of menaquinone-7. The total production and secretion rate of menaquinone-7 increased by 48.0% and 56.2% respectively. During fermentation, the integrity of the cell membrane decreased by 82.9% while the permeability increased by 158% when the maximum secretory rate was reached. Furthermore, Brij-58 supplementation induced the stress response in bacteria, resulting in hyperpolarization of the membrane, and increased membrane ATPase activity. Finally, changes in fatty acid composition increased membrane fluidity by 30.1%. This study provided an effective strategy to enhance menaquinone-7 yield in Bacillus natto and revealed the mechanism of Brij-58 supplementation in menaquinone-7 production.
Key points
• MK-7 yield in Bacillus natto was significantly increased by Brij-58 supplementation.
• Brij-58 could be adsorbed on cell surface and change fermentation environment.
• Brij-58 supplementation could affect the state and composition of the cell membrane.
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Data availability
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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This work was supported by the State Key Laboratory of Applied Microbiology Southern China (SKLAM010-2021), and the 111 Project (B17018).
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YY and XJ designed the study, conducted experiments and wrote the original manuscript. YY, MC and HY analyzed data and contributed with the analytical tools. TEC and HZ reviewed and edited the manuscript. HZ contributed to project supervision and advice. All authors read and approved the manuscript.
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Yi, Y., Jin, X., Chen, M. et al. Brij-58 supplementation enhances menaquinone-7 biosynthesis and secretion in Bacillus natto. Appl Microbiol Biotechnol 107, 5051–5062 (2023). https://doi.org/10.1007/s00253-023-12640-y
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DOI: https://doi.org/10.1007/s00253-023-12640-y