Abstract
In this study, loquat extract was selected as a promising substrate for bacterial cellulose (BC) production. A new BC-producing bacterial strain was isolated from residual loquat and identified as Komagataeibacter rhaeticus. BC production with different carbon sources and with loquat extract was investigated. Among all tested carbon sources, glucose was demonstrated to be the best substrate for BC production by K. rhaeticus, with up to 7.89 g/L dry BC obtained under the optimal initial pH (5.5) and temperature (28 °C) with 10 days of fermentation. The total sugar and individual sugars were investigated in different loquat extracts, in which fructose, glucose, and sucrose were the three main sugars. When loquat extract was prepared with a solid‒liquid (S-L) ratio of 2:1, the concentrations of glucose, fructose, and sucrose were 7.91 g/L, 9.31 g/L, and 2.84 g/L, respectively. The BC production obtained from loquat extract was higher than that of other carbon sources except glucose, and 6.69 g/L dry BC was obtained from loquat extract with an S-L ratio of 2:1. After BC production, all sugars substantially decreased, with the utilization of glucose, fructose, and sucrose reaching 93.9%, 87.9%, and 100%, respectively. These results suggested that the different sugars in loquat extract were all carbon sources participating in BC production by K. rhaeticus. Structural and physicochemical properties were investigated by SEM, TGA, XRD, and FT-IR spectroscopy. The results showed that the structural, chemical group, and water holding capacity of BC obtained from loquat extract were similar to those of BC obtained from glucose, but the crystallinity and thermal stability of BC were higher than those of BC from mannose and lactose but lower than those of BC from glucose and fructose.
Key points
• A new BC-producing strain was isolated and identified as Komagataeibacter rhaeticus.
• Loquat extract is an alternative substrate for BC production.
• The BC obtained from loquat extract owns advanced physicochemical properties.
Graphical Abstract
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Data availability
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Code availability
The sequence data of this study have been deposited in the NCBI database under accession number OP026093.
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Funding
The project was supported in part by the Doctor Support Grants of Putian University (2021068, 2021069) and the Natural Science Foundation of Fujian Province (2021J05242, 2021J05241, 2022J011167).
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Study concepts: JY, JX. Study design: JL, QW. Experimental studies: XW, SW. Data acquisition: HW, JL. Data analysis: JL, QW. Manuscript preparation: JY, JX. Manuscript revision: JX, HW.
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Ye, J., Li, J., Wang, Q. et al. Bacterial cellulose production by a strain of Komagataeibacter rhaeticus isolated from residual loquat. Appl Microbiol Biotechnol 107, 1551–1562 (2023). https://doi.org/10.1007/s00253-023-12407-5
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DOI: https://doi.org/10.1007/s00253-023-12407-5