Abstract
The depletion of Bacteroides in the gut is closely correlated with the progression of alcoholic liver disease (ALD). This study aimed to identify Bacteroides strains with protective effects against ALD and evaluate the synergistic effects of Bacteroides and pectin in this disease. Mice were fed Lieber-DeCarli alcohol diet to establish an experimental ALD model and pre-treated with 4 Bacteroides strains. The severity of the liver injury, hepatic steatosis, and inflammation was evaluated through histological and biochemical assays. We found that Bacteroides fragilis ATCC25285 had the best protective effects against ALD strains by alleviating both ethanol-induced liver injury and steatosis. B. fragilis ATCC25285 could counteract inflammatory reactions in ALD by producing short-chain fat acids (SCFAs) and enhancing the intestinal barrier. In the subsequent experiment, the synbiotic combination of B. fragilis ATCC25285 and pectin was evaluated and the underlying mechanisms were investigated by metabolomic and microbiome analyses. The combination elicited superior anti-ALD effects than the individual agents used alone. The synergistic effects of B. fragilis ATCC25285 and pectin were driven by modulating gut microbiota, improving tryptophan metabolism, and regulating intestinal immune function. Based on our findings, the combination of B. fragilis ATCC25285 and pectin can be considered a potential treatment for ALD.
Key points
• B. fragilis ATCC25285 was identified as a protective Bacteroides strain against ALD.
• The synbiotic combination of B. fragilis and pectin has better anti-ALD effects.
• The synbiotic combination modulates gut microbiota and tryptophan metabolism.
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Data availability
Gut microbiome 16S sequencing data were deposited in the Sequence Read Archive (SRA) database and can be accessed by the SRA accession number, PRJNA738382.
References
Albillos A, de Gottardi A, Rescigno M (2020) The gut-liver axis in liver disease: pathophysiological basis for therapy. J Hepatol 72(3):558–577. https://doi.org/10.1016/j.jhep.2019.10.003
Aßhauer KP, Wemheuer B, Daniel R, Meinicke P (2015) Tax4Fun: predicting functional profiles from metagenomic 16S rRNA data. Bioinformatics 31(17):2882–2884. https://doi.org/10.1093/bioinformatics/btv287
Avila MA, Dufour J-F, Gerbes AL, Zoulim F, Bataller R, Burra P, Cortez-Pinto H, Gao B, Gilmore I, Mathurin P, Moreno C, Poznyak V, Schnabl B, Szabo G, Thiele M, Thursz MR (2020) Recent advances in alcohol-related liver disease (ALD): summary of a Gut round table meeting. Gut 69(4):764–780. https://doi.org/10.1136/gutjnl-2019-319720
Bertola A, Mathews S, Ki SH, Wang H, Gao B (2013) Mouse model of chronic and binge ethanol feeding (the NIAAA model). Nat Protoc 8(3):627–637. https://doi.org/10.1038/nprot.2013.032
Bry L, Falk PG, Midtvedt T, Gordon JI (1996) A model of host-microbial interactions in an open mammalian ecosystem. Science 273(5280):1380–1383. https://doi.org/10.1126/science.273.5280.1380
Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Peña AG, Goodrich JK, Gordon JI, Huttley GA, Kelley ST, Knights D, Koenig JE, Ley RE, Lozupone CA, McDonald D, Muegge BD, Pirrung M, Reeder J, Sevinsky JR, Turnbaugh PJ, Walters WA, Widmann J, Yatsunenko T, Zaneveld J, Knight R (2010) QIIME allows analysis of high-throughput community sequencing data. Nat Methods 7(5):335–336. https://doi.org/10.1038/nmeth.f.303
Chan JL, Wu S, Geis AL, Chan GV, Gomes TAM, Beck SE, Wu X, Fan H, Tam AJ, Chung L, Ding H, Wang H, Pardoll DM, Housseau F, Sears CL (2019) Non-toxigenic Bacteroides fragilis (NTBF) administration reduces bacteria-driven chronic colitis and tumor development independent of polysaccharide A. Mucosal Immunol 12(1):164–177. https://doi.org/10.1038/s41385-018-0085-5
Chen G, Ran X, Li B, Li Y, He D, Huang B, Fu S, Liu J, Wang W (2018) Sodium butyrate inhibits inflammation and maintains epithelium barrier integrity in a tnbs-induced inflammatory bowel disease mice model. EBioMedicine 30:317–325. https://doi.org/10.1016/j.ebiom.2018.03.030
Cummings JH, Macfarlane GT, Englyst HN (2001) Prebiotic digestion and fermentation. Am J Clin Nutr 73(2 Suppl):415s–420s. https://doi.org/10.1093/ajcn/73.2.415s
Delday M, Mulder I, Logan ET, Grant G (2019) Bacteroides thetaiotaomicron ameliorates colon inflammation in preclinical models of Crohn’s disease. Inflamm Bowel Dis 25(1):85–96. https://doi.org/10.1093/ibd/izy281
Duan Y, Llorente C, Lang S, Brandl K, Chu H, Jiang L, White RC, Clarke TH, Nguyen K, Torralba M, Shao Y, Liu J, Hernandez-Morales A, Lessor L, Rahman IR, Miyamoto Y, Ly M, Gao B, Sun W, Kiesel R, Hutmacher F, Lee S, Ventura-Cots M, Bosques-Padilla F, Verna EC, Abraldes JG, Brown RS, Vargas V, Altamirano J, Caballería J, Shawcross DL, Ho SB, Louvet A, Lucey MR, Mathurin P, Garcia-Tsao G, Bataller R, Tu XM, Eckmann L, van der Donk WA, Young R, Lawley TD, Stärkel P, Pride D, Fouts DE, Schnabl B (2019) Bacteriophage targeting of gut bacterium attenuates alcoholic liver disease. Nature 575(7783):505–511. https://doi.org/10.1038/s41586-019-1742-x
Elshahed MS, Miron A, Aprotosoaie AC, Farag MA (2021) Pectin in diet: interactions with the human microbiome, role in gut homeostasis, and nutrient-drug interactions. Carbohydr Polym 255:117388. https://doi.org/10.1016/j.carbpol.2020.117388
Ferrere G, Wrzosek L, Cailleux F, Turpin W, Puchois V, Spatz M, Ciocan D, Rainteau D, Humbert L, Hugot C, Gaudin F, Noordine M-L, Robert V, Berrebi D, Thomas M, Naveau S, Perlemuter G, Cassard A-M (2017) Fecal microbiota manipulation prevents dysbiosis and alcohol-induced liver injury in mice. J Hepatol 66(4):806–815. https://doi.org/10.1016/j.jhep.2016.11.008
Fu X, Liu Z, Zhu C, Mou H, Kong Q (2019) Nondigestible carbohydrates, butyrate, and butyrate-producing bacteria. Crit Rev Food Sci Nutr 59(sup1):S130-s152. https://doi.org/10.1080/10408398.2018.1542587
Ghosh S, Whitley CS, Haribabu B, Jala VR (2021) Regulation of intestinal barrier function by microbial metabolites. Cell Mol Gastroenterol Hepatol 11(5):1463–1482. https://doi.org/10.1016/j.jcmgh.2021.02.007
Goldstein EJ (1996) Anaerobic bacteremia. Clin Infect Dis 23(Suppl 1):S97-101. https://doi.org/10.1093/clinids/23.supplement_1.s97
Grander C, Adolph TE, Wieser V, Lowe P, Wrzosek L, Gyongyosi B, Ward DV, Grabherr F, Gerner RR, Pfister A, Enrich B, Ciocan D, Macheiner S, Mayr L, Drach M, Moser P, Moschen AR, Perlemuter G, Szabo G, Cassard AM, Tilg H (2018) Recovery of ethanol-induced Akkermansia muciniphila depletion ameliorates alcoholic liver disease. Gut 67(5):891–901. https://doi.org/10.1136/gutjnl-2016-313432
Hamer HM, Jonkers D, Venema K, Vanhoutvin S, Troost FJ, Brummer RJ (2008) Review article: the role of butyrate on colonic function. Aliment Pharmacol Ther 27(2):104–119. https://doi.org/10.1111/j.1365-2036.2007.03562.x
Hendrikx T, Duan Y, Wang Y, Oh J-H, Alexander LM, Huang W, Stärkel P, Ho SB, Gao B, Fiehn O, Emond P, Sokol H, van Pijkeren J-P, Schnabl B (2019) Bacteria engineered to produce IL-22 in intestine induce expression of REG3G to reduce ethanol-induced liver disease in mice. Gut 68(8):1504–1515. https://doi.org/10.1136/gutjnl-2018-317232
Hong Y, Sheng L, Zhong J, Tao X, Zhu W, Ma J, Yan J, Zhao A, Zheng X, Wu G, Li B, Han B, Ding K, Zheng N, Jia W, Li H (2021) Desulfovibrio vulgaris, a potent acetic acid-producing bacterium, attenuates nonalcoholic fatty liver disease in mice. Gut Microbes 13(1):1–20. https://doi.org/10.1080/19490976.2021.1930874
Hooper LV, Midtvedt T, Gordon JI (2002) How host-microbial interactions shape the nutrient environment of the mammalian intestine. Annu Rev Nutr 22:283–307. https://doi.org/10.1146/annurev.nutr.22.011602.092259
Hosseini E, Grootaert C, Verstraete W, Van de Wiele T (2011) Propionate as a health-promoting microbial metabolite in the human gut. Nutr Rev 69(5):245–258. https://doi.org/10.1111/j.1753-4887.2011.00388.x
Hritz I, Mandrekar P, Velayudham A, Catalano D, Dolganiuc A, Kodys K, Kurt-Jones E, Szabo G (2008) The critical role of toll-like receptor (TLR) 4 in alcoholic liver disease is independent of the common TLR adapter MyD88. Hepatology 48(4):1224–1231. https://doi.org/10.1002/hep.22470
Ihekweazu FD, Fofanova TY, Queliza K, Nagy-Szakal D, Stewart CJ, Engevik MA, Hulten KG, Tatevian N, Graham DY, Versalovic J, Petrosino JF, Kellermayer R (2019) ATCC 8483 monotherapy is superior to traditional fecal transplant and multi-strain bacteriotherapy in a murine colitis model. Gut Microbes 10(4):504–520. https://doi.org/10.1080/19490976.2018.1560753
Jalili V, Afgan E, Gu Q, Clements D, Blankenberg D, Goecks J, Taylor J, Nekrutenko A (2020) The Galaxy platform for accessible, reproducible and collaborative biomedical analyses: 2020 update. Nucleic Acids Res 48(W1):W395-w402. https://doi.org/10.1093/nar/gkaa434
Jiang X-W, Li Y-T, Ye J-Z, Lv L-X, Yang L-Y, Bian X-Y, Wu W-R, Wu J-J, Shi D, Wang Q, Fang D-Q, Wang K-C, Wang Q-Q, Lu Y-M, Xie J-J, Li L-J (2020) New strain of Pediococcus pentosaceus alleviates ethanol-induced liver injury by modulating the gut microbiota and short-chain fatty acid metabolism. World J Gastroentero 26(40):6224–6240. https://doi.org/10.3748/wjg.v26.i40.6224
Keir M, Yi Y, Lu T, Ghilardi N (2020) The role of IL-22 in intestinal health and disease. J EXP MED 217(3):e20192195. https://doi.org/10.1084/jem.20192195
Kondo T, Kishi M, Fushimi T, Kaga T (2009) Acetic acid upregulates the expression of genes for fatty acid oxidation enzymes in liver to suppress body fat accumulation. J Agric Food Chem 57(13):5982–5986. https://doi.org/10.1021/jf900470c
Lee SM, Donaldson GP, Mikulski Z, Boyajian S, Ley K, Mazmanian SK (2013) Bacterial colonization factors control specificity and stability of the gut microbiota. Nature 501(7467):426–429. https://doi.org/10.1038/nature12447
Liu YX, Qin Y, Chen T, Lu M, Qian X, Guo X, Bai Y (2021) A practical guide to amplicon and metagenomic analysis of microbiome data. Protein Cell 12(5):315–330. https://doi.org/10.1007/s13238-020-00724-8
Llopis M, Cassard AM, Wrzosek L, Boschat L, Bruneau A, Ferrere G, Puchois V, Martin JC, Lepage P, Le Roy T, Lefèvre L, Langelier B, Cailleux F, González-Castro AM, Rabot S, Gaudin F, Agostini H, Prévot S, Berrebi D, Ciocan D, Jousse C, Naveau S, Gérard P, Perlemuter G (2016) Intestinal microbiota contributes to individual susceptibility to alcoholic liver disease. Gut 65(5):830–839. https://doi.org/10.1136/gutjnl-2015-310585
Luis AS, Briggs J, Zhang X, Farnell B, Ndeh D, Labourel A, Baslé A, Cartmell A, Terrapon N, Stott K, Lowe EC, McLean R, Shearer K, Schückel J, Venditto I, Ralet M-C, Henrissat B, Martens EC, Mosimann SC, Abbott DW, Gilbert HJ (2018) Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. Nat Microbiol 3(2):210–219. https://doi.org/10.1038/s41564-017-0079-1
Mazmanian SK, Round JL, Kasper DL (2008) A microbial symbiosis factor prevents intestinal inflammatory disease. Nature 453(7195):620–625. https://doi.org/10.1038/nature07008
Parks OB, Pociask DA, Hodzic Z, Kolls JK, Good M (2015) Interleukin-22 signaling in the regulation of intestinal health and disease. Front Cell Dev Biol 3:85. https://doi.org/10.3389/fcell.2015.00085
Qian M, Liu J, Zhao D, Cai P, Pan C, Jia W, Gao Y, Zhang Y, Zhang N, Zhang Y, Zhang Q, Wu D, Shan C, Zhang M, Schnabl B, Yang S, Shen X, Wang L (2021) Aryl hydrocarbon receptor deficiency in intestinal epithelial cells aggravates alcohol-related liver disease. Cell Mol Gastroenterol Hepatol. https://doi.org/10.1016/j.jcmgh.2021.08.014
Ridlon JM, Devendran S, Alves JM, Doden H, Wolf PG, Pereira GV, Ly L, Volland A, Takei H, Nittono H, Murai T, Kurosawa T, Chlipala GE, Green SJ, Hernandez AG, Fields CJ, Wright CL, Kakiyama G, Cann I, Kashyap P, McCracken V, Gaskins HR (2020) The “in vivo lifestyle” of bile acid 7α-dehydroxylating bacteria: comparative genomics, metatranscriptomic, and bile acid metabolomics analysis of a defined microbial community in gnotobiotic mice. Gut Microbes 11(3):381–404. https://doi.org/10.1080/19490976.2019.1618173
Roager HM, Licht TR (2018) Microbial tryptophan catabolites in health and disease. Nat Commun 9(1):3294. https://doi.org/10.1038/s41467-018-05470-4
Roediger WE (1980) Role of anaerobic bacteria in the metabolic welfare of the colonic mucosa in man. Gut 21(9):793–798. https://doi.org/10.1136/gut.21.9.793
Sarin SK, Pande A, Schnabl B (2019) Microbiome as a therapeutic target in alcohol-related liver disease. J Hepatol 70(2):260–272. https://doi.org/10.1016/j.jhep.2018.10.019
Schwalm ND, Groisman EA (2017) Navigating the gut buffet: control of polysaccharide utilization in Bacteroides spp. Trends Microbiol 25(12):1005–1015. https://doi.org/10.1016/j.tim.2017.06.009
Sehrawat TS, Liu M, Shah VH (2020) The knowns and unknowns of treatment for alcoholic hepatitis. Lancet Gastroenterol Hepatol 5(5):494–506. https://doi.org/10.1016/S2468-1253(19)30326-7
Seo B, Jeon K, Moon S, Lee K, Kim W-K, Jeong H, Cha KH, Lim MY, Kang W, Kweon M-N, Sung J, Kim W, Park J-H, Ko G (2020) Roseburia spp. abundance associates with alcohol consumption in humans and its administration ameliorates alcoholic fatty liver in mice. Cell Host Microbe 27(1):25–40.e6. https://doi.org/10.1016/j.chom.2019.11.001
Shen Y, Giardino Torchia ML, Lawson GW, Karp CL, Ashwell JD, Mazmanian SK (2012) Outer membrane vesicles of a human commensal mediate immune regulation and disease protection. Cell Host Microbe 12(4):509–520. https://doi.org/10.1016/j.chom.2012.08.004
Singh V, Yeoh BS, Chassaing B, Xiao X, Saha P, Aguilera Olvera R, Lapek JD Jr, Zhang L, Wang WB, Hao S, Flythe MD, Gonzalez DJ, Cani PD, Conejo-Garcia JR, Xiong N, Kennett MJ, Joe B, Patterson AD, Gewirtz AT, Vijay-Kumar M (2018) Dysregulated microbial fermentation of soluble fiber induces cholestatic liver cancer. Cell 175(3):679-694.e22. https://doi.org/10.1016/j.cell.2018.09.004
Singhal R, Donde H, Ghare S, Stocke K, Zhang J, Vadhanam M, Reddy S, Gobejishvili L, Chilton P, Joshi-Barve S, Feng W, McClain C, Hoffman K, Petrosino J, Vital M, Barve S (2021) Decrease in acetyl-CoA pathway utilizing butyrate-producing bacteria is a key pathogenic feature of alcohol-induced functional gut microbial dysbiosis and development of liver disease in mice. Gut Microbes 13(1):1946367. https://doi.org/10.1080/19490976.2021.1946367
Sofi MH, Wu Y, Ticer T, Schutt S, Bastian D, Choi H-J, Tian L, Mealer C, Liu C, Westwater C, Armeson KE, Alekseyenko AV, Yu X-Z (2021) A single strain of Bacteroides fragilis protects gut integrity and reduces GVHD. JCI insight 6(3):e136841. https://doi.org/10.1172/jci.insight.136841
Swanson KS, Gibson GR, Hutkins R, Reimer RA, Reid G, Verbeke K, Scott KP, Holscher HD, Azad MB, Delzenne NM, Sanders ME (2020) The international scientific association for probiotics and prebiotics (ISAPP) consensus statement on the definition and scope of synbiotics. Nat Rev Gastroenterol Hepatol 17(11):687–701. https://doi.org/10.1038/s41575-020-0344-2
Tsuruya A, Kuwahara A, Saito Y, Yamaguchi H, Tsubo T, Suga S, Inai M, Aoki Y, Takahashi S, Tsutsumi E, Suwa Y, Morita H, Kinoshita K, Totsuka Y, Suda W, Oshima K, Hattori M, Mizukami T, Yokoyama A, Shimoyama T, Nakayama T (2016) Ecophysiological consequences of alcoholism on human gut microbiota: implications for ethanol-related pathogenesis of colon cancer. Sci Rep 6:27923. https://doi.org/10.1038/srep27923
Wang L, Fouts DE, Stärkel P, Hartmann P, Chen P, Llorente C, DePew J, Moncera K, Ho SB, Brenner DA, Hooper LV, Schnabl B (2016) Intestinal REG3 lectins protect against alcoholic steatohepatitis by reducing mucosa-associated microbiota and preventing bacterial translocation. Cell Host Microbe 19(2):227–239. https://doi.org/10.1016/j.chom.2016.01.003
Wang R, Tang R, Li B, Ma X, Schnabl B, Tilg H (2021) Gut microbiome, liver immunology, and liver diseases. Cell Mol Immunol 18(1):4–17. https://doi.org/10.1038/s41423-020-00592-6
Wang S, Yang L, Hu H, Lv L, Ji Z, Zhao Y, Zhang H, Xu M, Fang R, Zheng L, Ding C, Yang M, Xu K, Li L (2022) Characteristic gut microbiota and metabolic changes in patients with pulmonary tuberculosis. Microb Biotechnol 15(1):262–275. https://doi.org/10.1111/1751-7915.13761
Weckx S, Van der Meulen R, Maes D, Scheirlinck I, Huys G, Vandamme P, De Vuyst L (2010) Lactic acid bacteria community dynamics and metabolite production of rye sourdough fermentations share characteristics of wheat and spelt sourdough fermentations. Food Microbiol 27(8):1000–1008. https://doi.org/10.1016/j.fm.2010.06.005
Wexler AG, Goodman AL (2017) An insider’s perspective: Bacteroides as a window into the microbiome. Nat Microbiol 2:17026. https://doi.org/10.1038/nmicrobiol.2017.26
Wexler HM (2007) Bacteroides: the good, the bad, and the nitty-gritty. Clin Microbiol Rev 20(4):593–621
Willemsen LE, Koetsier MA, van Deventer SJ, van Tol EA (2003) Short chain fatty acids stimulate epithelial mucin 2 expression through differential effects on prostaglandin E(1) and E(2) production by intestinal myofibroblasts. Gut 52(10):1442–1447. https://doi.org/10.1136/gut.52.10.1442
Wlodarska M, Luo C, Kolde R, d’Hennezel E, Annand JW, Heim CE, Krastel P, Schmitt EK, Omar AS, Creasey EA, Garner AL, Mohammadi S, O’Connell DJ, Abubucker S, Arthur TD, Franzosa EA, Huttenhower C, Murphy LO, Haiser HJ, Vlamakis H, Porter JA, Xavier RJ (2017) Indoleacrylic acid produced by commensal peptostreptococcus species suppresses inflammation. Cell Host Microbe 22(1):25-37.e6. https://doi.org/10.1016/j.chom.2017.06.007
Wrzosek L, Ciocan D, Hugot C, Spatz M, Dupeux M, Houron C, Lievin-Le Moal V, Puchois V, Ferrere G, Trainel N, Mercier-Nomé F, Durand S, Kroemer G, Voican CS, Emond P, Straube M, Sokol H, Perlemuter G, Cassard A-M (2020) Microbiota tryptophan metabolism induces aryl hydrocarbon receptor activation and improves alcohol-induced liver injury. Gut. https://doi.org/10.1136/gutjnl-2020-321565
Wu S, Lim KC, Huang J, Saidi RF, Sears CL (1998) Bacteroides fragilis enterotoxin cleaves the zonula adherens protein, E-cadherin. Proc Natl Acad Sci U S A 95(25):14979–14984. https://doi.org/10.1073/pnas.95.25.14979
Xie G, Zhong W, Zheng X, Li Q, Qiu Y, Li H, Chen H, Zhou Z, Jia W (2013) Chronic ethanol consumption alters mammalian gastrointestinal content metabolites. J Proteome Res 12(7):3297–3306. https://doi.org/10.1021/pr400362z
Xu Y, Zhu J, Feng B, Lin F, Zhou J, Liu J, Shi X, Lu X, Pan Q, Yu J, Zhang Y, Li L, Cao H (2021) Immunosuppressive effect of mesenchymal stem cells on lung and gut CD8(+) T cells in lipopolysaccharide-induced acute lung injury in mice. Cell Prolif 54(5):e13028. https://doi.org/10.1111/cpr.13028
Ye J, Lv L, Wu W, Li Y, Shi D, Fang D, Guo F, Jiang H, Yan R, Ye W, Li L (2018) Butyrate protects mice against methionine-choline-deficient diet-induced non-alcoholic steatohepatitis by improving gut barrier function, attenuating inflammation and reducing endotoxin levels. Front Microbiol 9:1967. https://doi.org/10.3389/fmicb.2018.01967
Zaharie RD, Popa C, Schlanger D, Vălean D, Zaharie F (2022) The role of IL-22 in wound healing. potential implications in clinical practice. Int J Mol Sci 23(7):3693. https://doi.org/10.3390/ijms23073693
Zeisel MB, Dhawan P, Baumert TF (2019) Tight junction proteins in gastrointestinal and liver disease. Gut 68(3):547–561. https://doi.org/10.1136/gutjnl-2018-316906
Zhang J, Zhu S, Ma N, Johnston LJ, Wu C, Ma X (2020) Metabolites of microbiota response to tryptophan and intestinal mucosal immunity: a therapeutic target to control intestinal inflammation. Med Res Rev. https://doi.org/10.1002/med.21752
Acknowledgements
We thank the laboratory animal center of the First Affiliated Hospital of Zhejiang University for helping with the maintenance of SPF mice.
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This work was supported by the National Natural Science Foundation of China under Grant [81790631, 81570512]; the National Key Research and Development Program of China under Grant [2018YFC2000500, 2021YFA1301104, 2021YFC2301804]; the Research Project of Jinan Microecological Biomedicine Shandong Laboratory (JNL-2022001A); and Natural Science Foundation of Zhejiang Province, China under Grant [LQ19H030007].
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QQW, YTL, and LJL conceived and designed the experiments. QQW, STW, YML, ZJW, JS, SMJ, JWL, SJL, and AXZG conducted the experiments and collected the samples. LJL, LXL, HYJ, and RY offered resources for the experiments. QQW and RY analyzed the data. QQW and YTL wrote the paper. All the authors have read and approved the manuscript.
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Wang, Q., Li, Y., Lv, L. et al. Identification of a protective Bacteroides strain of alcoholic liver disease and its synergistic effect with pectin. Appl Microbiol Biotechnol 106, 3735–3749 (2022). https://doi.org/10.1007/s00253-022-11946-7
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DOI: https://doi.org/10.1007/s00253-022-11946-7