Abstract
This work performed co-AD from the vinasse and filter cake (from 1G ethanol production) and deacetylation liquor (from the pretreatment of sugarcane straw for 2G ethanol production) in a semi-Continuous Stirred Tank Reactor (s-CSTR) aiming to provide optimum operational parameters for continuous CH4 production. Using filter cake as co-substrate may allow the reactor to operate throughout the year, as it is available in the sugarcane off-season, unlike vinasse. A comparison was made from the microbial community of the seed sludge and the reactor sludge when CH4 production stabilized. Lactate, butyrate, and propionate fermentation routes were denoted at the start-up of the s-CSTR, characterizing the acidogenic phase: the oxidation-reduction potential (ORP) values ranged from −800 to −100 mV. Once the methanogenesis was initiated, alkalizing addition was no longer needed as its demand by the microorganisms was supplied by the alkali characteristics of the deacetylation liquor. The gradual increase of the applied organic load rates (OLR) allowed stabilization of the methanogenesis from 3.20 gVS L−1 day−1: the highest CH4 yield (230 mLNCH4 g−1VS) and average organic matter removal efficiency (83% ± 13) was achieved at ORL of 4.16 gVS L−1 day−1. The microbial community changed along with the reactor operation, presenting different metabolic routes mainly due to the used lignocellulosic substrates. Bacteria from the syntrophic acetate oxidation (SAO) process coupled to hydrogenotrophic methanogenesis were predominant (~ 90% Methanoculleus) during the CH4 production stability. The overall results are useful as preliminary drivers in terms of visualizing the co-AD process in a sugarcane biorefinery integrated to scale.
Key points
• Integration of 1G2G sugarcane ethanol biorefinery from co-digestion of its residues.
• Biogas production from vinasse, filter cake, and deacetylation liquor in a semi-CSTR.
• Lignocellulosic substrates affected the biochemical routes and microbial community.
• Biomol confirmed the establishment of the thermophilic community from mesophilic sludge.
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Data availability
The datasets generated during and/r analyzed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
The authors gratefully acknowledge the support of the Laboratory of Environment and Sanitation (LMAS) at the School of Agricultural Engineering (FEAGRI/UNICAMP), the National Laboratory of Biorenewables (LNBR/CNPEM) and the Interdisciplinary Center of Energy Planning (NIPE/UNICAMP).
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This work was supported by São Paulo Research Foundation - FAPESP contract numbers 2018/09893-1 to MPCV, 2016/16438-3 to BSM, and 2015/50612-8 to TTF (FAPESP-BBSRC Thematic Project).
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MPCV: conceptualization, investigation, methodology, data curation, and writing-original draft preparation. ADFNJ: methodology, resources, data curation, and writing–original draft preparation. TTF: project administration and funding acquisition. BSM: conceptualization, formal analysis, writing–review and editing, supervision, and funding acquisition. All authors read and approved the manuscript.
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Volpi, M.P.C., Junior, A.D.N.F., Franco, T.T. et al. Operational and biochemical aspects of co-digestion (co-AD) from sugarcane vinasse, filter cake, and deacetylation liquor. Appl Microbiol Biotechnol 105, 8969–8987 (2021). https://doi.org/10.1007/s00253-021-11635-x
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DOI: https://doi.org/10.1007/s00253-021-11635-x