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Glycosyl hydrolase catalyzed glycosylation in unconventional media

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Abstract

The reversible hydrolytic property of glycosyl hydrolases (GHs) as well as their acceptance of aglycones other than water has provided the abilities of GHs in synthesizing glycosides. Together with desirable physiochemical properties of glycosides and their high commercial values, research interests have been aroused to investigate the synthetic other than the hydrolytic properties of GHs. On the other hand, just like the esterification processes catalyzed by lipases, GH synthetic effectiveness is strongly obstructed by water both thermodynamically and kinetically. Medium engineering by involving organic solvents can be a viable approach to alleviate the obstacles caused by water. However, as native hydrolyases function in water-enriched environments, most GHs display poor catalytic performance in the presence of organic solvents. Some GHs from thermophiles are more tolerant to organic solvents due to their robust folded structures with strong residue interactions. Other than native sources, immobilization, protein engineering, employment of surfactant, and lyophilization have been proved to enhance the GH stability from the native state, which opens up the possibilities for GHs to be employed in unconventional media as synthases.

Key points

• Unconventional media enhance the synthetic ability but destabilize GHs.

• Viable approaches are discussed to improve GH stability from the native state.

• GHs robust in unconventional media can be valuable industrial synthases.

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Acknowledgments

We are grateful to the National Natural Science Foundation of China and Key Research & Development in Zhejiang in encouraging us for the studies in the glycosylation area.

Funding

This study was funded by the National Natural Science Foundation of China (No. 21908196) and Key Research & Development in Zhejiang (No. 2019C01085).

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Authors and Affiliations

  1. Fermentation Technology Institute, Zhejiang University of Technology, Hangzhou, 310014, China

    Hanchi Chen, Xiao Jin, Linjiang Zhu, Yuele Lu, Zhi Ma & Xiaolong Chen

  2. College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China

    Hanchi Chen, Xiao Jin, Linjiang Zhu, Yuele Lu, Zhi Ma & Xiaolong Chen

  3. Department of Paper and Bioprocess Engineering, SUNY-College of Environmental Science and Forestry, Syracuse, NY, 13210, USA

    Shijie Liu

Authors
  1. Hanchi Chen
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  2. Xiao Jin
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  3. Linjiang Zhu
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  4. Yuele Lu
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  5. Zhi Ma
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  6. Shijie Liu
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  7. Xiaolong Chen
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Contributions

All authors have their own contributions to this work. The first author, Dr. Hanchi Chen, was in charge of the study including writing the manuscript. Ms. Xiao Jin has been helped in collecting and organizing materials. Dr. Linjiang Zhu, Dr. Yuele Lu, Dr. Zhi Ma, and Dr. Shijie Liu have been providing valuable advices. Dr. Xiaolong Chen has founded the whole study as well as providing research advices.

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Correspondence to Xiaolong Chen.

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Chen, H., Jin, X., Zhu, L. et al. Glycosyl hydrolase catalyzed glycosylation in unconventional media. Appl Microbiol Biotechnol 104, 9523–9534 (2020). https://doi.org/10.1007/s00253-020-10924-1

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  • DOI: https://doi.org/10.1007/s00253-020-10924-1

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