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Unraveling the proteomic changes involved in the resistance response of Cajanus platycarpus to herbivory by Helicoverpa armigera

  • Genomics, transcriptomics, proteomics
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

The pigeonpea wild relative Cajanus platycarpus is resistant to Helicoverpa armigera, one of the major pests responsible for yield losses in Cajanus cajan. Deciphering the molecular mechanism underlying host plant resistance is pertinent to identify proteins that aid in the mitigation of the insect pest. The present study adopted comparative proteomics as a tool to interpret the resistance mechanism(s) in C. platycarpus vis-à-vis C. cajan during continued herbivory (up to 96 h). Over-representation analysis of the differentially expressed proteins implicated a multi-dimensional resistance response accomplished by both physical and chemical barriers in C. platycarpus. While the chemical basis for resistance was depicted by the upregulation of proteins playing a rate limiting role in the phenylpropanoid pathway, the physical basis was provided by the regulation of proteins involved in microtubule assembly and synthesis of lignins. Upregulation of proteins in the polyamine pathway indicated the role of metabolite conjugates to be negatively affecting herbivore growth. Reallocation of resources and diversion of metabolic flux to support the production of secondary metabolites could be the probable approach in the wild relative against herbivory. Our study provided deeper insights into the pod borer resistance mechanism in C. platycarpus for utility in crop improvement.

Key points

• Pod borer resistance in Cajanus platycarpus is multi-dimensional.

• Pod borer resistance has been arbitrated to cell wall rigidity and secondary metabolites.

• Phenylpropanoid pathway derivatives apparently shaped the plant chemical defense against pod borer.

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Funding

The study was financially supported by DBT-Indo Swiss Collaboration in Biotechnology.

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Authors and Affiliations

  1. ICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi, India

    Maniraj Rathinam, Pragya Mishra, Shaily Tyagi & Rohini Sreevathsa

  2. Functional Genomics Center Zürich, University of Zurich/ETH Zurich, Winterthurerstrasse 190, CH-8057, Zürich, Switzerland

    Bernd Roschitzki, Jonas Grossmann, Laura Kunz, Witold Wolski, Christian Panse & Ralph Schlapbach

  3. Swiss Institute of Bioinformatics, Quartier Sorge - Batiment Amphipole, 1015, Lausanne, Switzerland

    Jonas Grossmann, Witold Wolski & Christian Panse

  4. Division of Nematology, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi, India

    Uma Rao

Authors
  1. Maniraj Rathinam
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  11. Rohini Sreevathsa
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Contributions

RoS, BR, UR conceived and designed the experiments; MR, PM conducted the herbivore challenge experiment and extracted the total proteins; BR, LK performed mass spectrometry and data acquisition; JG, WW, CP developed local data base and performed the protein quantification and identification; MR, JG, PM, ST performed downstream analysis and validated the proteome data; MR, BR, JG and RoS wrote the manuscript; RoS, UR, RaS critically reviewed and edited the manuscript. All authors read and approved the final manuscript.

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Correspondence to Rohini Sreevathsa.

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Rathinam, M., Roschitzki, B., Grossmann, J. et al. Unraveling the proteomic changes involved in the resistance response of Cajanus platycarpus to herbivory by Helicoverpa armigera. Appl Microbiol Biotechnol 104, 7603–7618 (2020). https://doi.org/10.1007/s00253-020-10787-6

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