Abstract
Human tissue plasminogen activator was the first recombinant therapy protein that successfully produced in Chinese hamster ovary cells in 1986 and approved for clinical use. Since then, more and more therapeutic proteins are being manufactured in mammalian cells, and the technologies for recombinant protein production in this expression system have developed rapidly, with the optimization of both upstream and downstream processes. One of the most promising strategies is expression vector cassette optimization based on the expression vector cassette. In this review paper, these approaches and developments are summarized, and the future strategy on the utilizing of expression cassettes for the production of recombinant therapeutic proteins in mammalian cells is discussed.
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Funding
This work was supported by the Grants from Basic Research Foundation of Key Scientific Research of Universities in Henan Province (No. 20zx013), Major Science and Technology projects of Henan Province (No. 191110311500), the Key Scientific Research Projects in Universities of Henan Province (No. 19A350008).
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Xiao Guo reviewed and wrote the manuscript. Tian-Yun Wang designed, analyzed, and co-wrote the manuscript.
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Key points
Expression vector cassette engineering in mammalian cell systems is reviewed.
Vector engineering can improve the expression of recombinant protein.
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Wang, TY., Guo, X. Expression vector cassette engineering for recombinant therapeutic production in mammalian cell systems. Appl Microbiol Biotechnol 104, 5673–5688 (2020). https://doi.org/10.1007/s00253-020-10640-w
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DOI: https://doi.org/10.1007/s00253-020-10640-w