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Synergistic effect of Lactobacillus gasseri and Cudrania tricuspidata on the modulation of body weight and gut microbiota structure in diet-induced obese mice

  • Applied microbial and cell physiology
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Abstract

High-fat diet (HFD)-induced obesity has been associated with alteration of gut microbiota alongside body weight gain. In this study, the synbiotic effect of Lactobacillus gasseri 505 (LG) and Cudrania tricuspidata (CT) in HFD-induced mice was revealed. After feeding mice with high-fat diet for 10 weeks, combination of LG and CT (LG_CT) exhibited the greatest reduction in the final body weight (11.9%). Moreover, microbial diversity significantly increased, and Principal Coordinate Analysis (PCoA) revealed that the LG_CT group showed closer cluster to NORM. At phylum level, the Firmicutes/Bacteroidetes (F/B) ratio increased in HFD, and the abundance of Bacteroidetes was restored by LG and CT. At genus level, notable changes in Alistipes, Desulfovibrio, Bilophila, and Acetatifactor were observed. Helicobacter elevated to 16.2% in HFD and diminished dramatically to less than 0.01% in LG and/or CT. At species level, L. gasseri increased after the administration of LG (0.54%) and LG_CT (1.14%), suggesting that LG may grow and colonize in the gut and CT can function as a prebiotic. Finally, functional analysis revealed certain metabolic factors correlated with body weight and gut microbiota. This study serves as a potential basis for the application of L. gasseri 505 and C. tricuspidata in the prevention and treatment of diet-induced obesity.

Key Points

• Combination of L. gasseri (LG) and C. tricuspidata (CT) reduced body weight gain.

• Microbial diversity significantly increased in LG_CT treatment.

• Abundance of microorganisms involved with leanness increased in LG, CT, and LG_CT.

• Body weight is associated with some metabolic functions of gut microbiota.

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Availability of data and materials

All standard sequence format (.fastq) files generated by Illumina Miseq containing all raw sequence reads have been deposited at the National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA): SRP201570 (https://trace.ncbi.nlm.nih.gov/Traces/sra/?study=SRP201570).

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Funding

This work was supported by Seoul Dairy Cooperative (Ansan, Republic of Korea). In addition, this work was also supported by a grant from the Next-Generation BioGreen 21 Program (Project No. PJ01322303), Rural Development Administration, Republic of Korea.

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  1. Nam Su Oh

    Present address: Department of Food and Biotechnology, Korea University Sejong Campus, Sejong, 30019, Republic of Korea

  2. Ji Young Lee

    Present address: Institute of Advanced Technology, CJ Cheiljedang Co., Suwon, 16495, Republic of Korea

Authors and Affiliations

  1. Department of Animal Resources Science, Dankook University, 119 Dandae-ro, Cheonan, 31116, Republic of Korea

    Ju Kyoung Oh, Mia Beatriz C. Amoranto, Ye Na Oh & Dae-Kyung Kang

  2. R&D Center, Seoul Dairy Cooperative, Ansan, 15407, Republic of Korea

    Nam Su Oh, Ji Young Lee & Yong Kook Shin

  3. Department of Food and Biotechnology, Korea University Sejong Campus, Sejong, 30019, Republic of Korea

    Yohan Yoon

  4. Department of Food and Nutrition, Sookmyung Women’s University, Seoul, 04310, Republic of Korea

    Sejeong Kim

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Contributions

D-KK, YKS, YY, and NSO designed the experiments. JKO, SK, JYL, and YNO performed experiments and analyzed the data. D-KK, JKO, and MBCA wrote the paper. All authors edited the manuscript and approved the final version.

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Correspondence to Dae-Kyung Kang.

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Oh, J.K., Amoranto, M.B.C., Oh, N.S. et al. Synergistic effect of Lactobacillus gasseri and Cudrania tricuspidata on the modulation of body weight and gut microbiota structure in diet-induced obese mice. Appl Microbiol Biotechnol 104, 6273–6285 (2020). https://doi.org/10.1007/s00253-020-10634-8

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