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A novel whole-cell biosensor of Pseudomonas aeruginosa to monitor the expression of quorum sensing genes

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Abstract

A novel whole-cell biosensor was developed to noninvasively and simultaneously monitor the in situ genetic activities of the four quorum sensing (QS) networks in Pseudomonas aeruginosa PAO1, including the las, rhl, pqs, and iqs systems. P. aeruginosa PAO1 is a model bacterium for studies of biofilm and pathogenesis while both processes are closely controlled by the QS systems. This biosensor worked well by selectively monitoring the expression of one representative gene from each network. In the biosensor, the promoter regions of lasI, rhlI, pqsA, and ambB (QS genes) controlled the fluorescent reporter genes of Turbo YFP, mTag BFP2, mNEON Green, and E2-Orange, respectively. The biosensor was successful in monitoring the impact of an important environmental factor, salt stress, on the genetic regulation of QS networks. High salt concentrations (≥ 20 g·L−1) significantly downregulated rhlI, pqsA, and ambB after the biosensor was incubated for 17 h to 18 h at 37 °C, resulting in slow bacterial growth.

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Acknowledgements

pSEVA-P lac -M Cherry, pSEVA-P lac -Turbo YFP, pSEVA-P lac -mNEON Green, pSEVA-P lac -E2-Orange, pSEVA-P lac -mTag BFP2 (Tables 1 and S1) and several other plasmids used in this study were constructed and generously donated by Dr. Patrick Billard (Université de Lorraine, CNRS, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), UMR 7360, Vandoeuvre-lès-Nancy, France). The authors highly appreciate his contribution and help.

Funding

This study was funded by the MIZZOU Advantage program at the University of Missouri (Columbia, MO, USA).

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Authors and Affiliations

  1. Department of Civil & Environmental Engineering, University of Missouri, Columbia, MO, USA

    Chiqian Zhang, Damien Parrello & Zhiqiang Hu

  2. Division of Biological Sciences, University of Missouri, Columbia, MO, USA

    Pamela J. B. Brown

  3. Department of Biochemistry, University of Missouri, Columbia, MO, USA

    Judy D. Wall

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  1. Chiqian Zhang
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Correspondence to Zhiqiang Hu.

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Zhang, C., Parrello, D., Brown, P.J.B. et al. A novel whole-cell biosensor of Pseudomonas aeruginosa to monitor the expression of quorum sensing genes. Appl Microbiol Biotechnol 102, 6023–6038 (2018). https://doi.org/10.1007/s00253-018-9044-z

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