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Microbial diversity and composition in different gut locations of hyperlipidemic mice receiving krill oil

  • Applied microbial and cell physiology
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Abstract

Low-dose (LD, 100 mg kg−1 day−1), moderate-dose (MD, 200 mg kg−1 day−1), and high-dose (HD, 600 mg kg−1 day−1) krill oil treatments have a stepwise, enhanced effect on alleviating hyperlipidemia, and 16S rRNA sequencing of the fecal samples demonstrates that krill oil treatment alters microbial communities. Feces may not represent all microbial communities in the gastrointestinal (GI) tract. Therefore, in this study, the stored ileal and colon samples collected from LD and HD groups were sequenced, and the location-specific modulations of microbial communities were observed after krill oil treatments. The 16S rRNA sequencing of the ileal samples showed that the LD and HD groups have similar patterns between control and high-fat diet (HFD) treatments, and six most abundant genera and 40 operational taxonomic units that respond to krill oil treatment were identified. However, the 16S rRNA sequencing of the colon samples showed that LD krill oil shifts the structure from the HFD to that of the control, whereas the HD group was distributed between the control and HFD groups. The corresponding most abundant genera and responsive OTUs totaled 4 and 45, respectively. In conclusion, different gastrointestinal tract locations contain different microbial communities. These results will help to provide a comprehensive understanding of the role of dietary krill oil in modulating the gut microbiota and alleviating hyperlipidemia.

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Acknowledgments

This work was supported by the Natural Science Foundation of Zhejiang Province (LY18C010001), General project of Zhejiang Education Department (Y20163580), K.C. Wong Magna Fund in Ningbo University. We thank Nature Research Editing Service for English language editing.

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Authors and Affiliations

  1. School of Marine Science, Ningbo University, Ningbo, 315211, China

    Chenyang Lu, Tingting Sun, Dijun Zhang, Jun Zhou & Xiurong Su

  2. Department of Food Science, Cornell University, Ithaca, New York, NY, USA

    Yanyan Li

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  1. Chenyang Lu
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  2. Tingting Sun
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Correspondence to Chenyang Lu.

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All experimental procedures and animal care were performed in accordance with the Guide for the Care and Use of Laboratory Animals prepared by the Ningbo University Laboratory Animal Center (affiliated with the Zhejiang Laboratory Animal Common Service Platform), and all the animal protocols were approved by the Ningbo University Laboratory Animal Center under permit number No. SYXK (ZHE 2008-0110).

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Lu, C., Sun, T., Li, Y. et al. Microbial diversity and composition in different gut locations of hyperlipidemic mice receiving krill oil. Appl Microbiol Biotechnol 102, 355–366 (2018). https://doi.org/10.1007/s00253-017-8601-1

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