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Fluorescence microscopic analysis of antifungal effects of cold atmospheric pressure plasma in Saccharomyces cerevisiae

  • Applied microbial and cell physiology
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Abstract

Cold atmospheric pressure plasma (CAP) has potential to be utilized as an alternative method for sterilization in food industries without thermal damage or toxic residues. In contrast to the bactericidal effects of CAP, information regarding the efficacy of CAP against eukaryotic microorganisms is very limited. Therefore, herein we investigated the effects of CAP on the budding yeast Saccharomyces cerevisiae, with a focus on the cellular response to CAP. The CAP treatment caused oxidative stress responses including the nuclear accumulation of the oxidative stress responsive transcription factor Yap1, mitochondrial fragmentation, and enhanced intracellular oxidation. Yeast cells also induced the expression of heat shock protein (HSP) genes and formation of Hsp104 aggregates when treated with CAP, suggesting that CAP denatures proteins. As phenomena unique to eukaryotic cells, the formation of cytoplasmic mRNP granules such as processing bodies and stress granules and changes in the intracellular localization of Ire1 were caused by the treatment with CAP, indicating that translational repression and endoplasmic reticulum (ER) stress were induced by the CAP treatment. These results suggest that the fungicidal effects of CAP are attributed to the multiple severe stresses.

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Acknowledgments

We are grateful to Drs. Y. Kimata, S. Kuge, and K. Okamoto for the yeast strains and plasmids and A. Yuri and Y. Sanada for the plasma sources.

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  1. Laboratory of Microbial Technology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Kyoto, 606-8585, Japan

    Koki Itooka & Shingo Izawa

  2. Electronic Material Science Laboratory, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Kyoto, 606-8585, Japan

    Kazuo Takahashi

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  1. Koki Itooka
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  2. Kazuo Takahashi
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  3. Shingo Izawa
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Correspondence to Shingo Izawa.

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This study was supported by a grant from the Japan Society for the Promotion of Science (No. 26,292,039).

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Itooka, K., Takahashi, K. & Izawa, S. Fluorescence microscopic analysis of antifungal effects of cold atmospheric pressure plasma in Saccharomyces cerevisiae . Appl Microbiol Biotechnol 100, 9295–9304 (2016). https://doi.org/10.1007/s00253-016-7783-2

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