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Genome-wide identification, expression analysis of GH3 family genes in Medicago truncatula under stress-related hormones and Sinorhizobium meliloti infection

  • Genomics, transcriptomics, proteomics
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Abstract

Auxin plays a pivotal role in the regulation of plant growth and development by controlling the expression of auxin response genes rapidly. As one of the major auxin early response gene families, Gretchen Hagen 3 (GH3) genes are involved in auxin homeostasis by conjugating excess auxins to amino acids. However, how GH3 genes function in environmental stresses and rhizobial infection responses in Medicago truncatula are largely unknown. Here, based on the latest updated M. truncatula genome, a comprehensive identification and expression profiling analysis of MtGH3 genes were performed. Our data showed that most of MtGH3 genes were expressed in tissue-specific manner and were responsive to environmental stress-related hormones. To understand the possible roles of MtGH3 genes involved in symbiosis establishment between M. truncatula and symbiotic bacteria, quantitative real-time polymerase chain reaction (qRT-PCR) was used to test the expressions of MtGH3 genes during the early phase of Sinorhizobium meliloti infection. The expression levels of most MtGH3 genes were upregulated in shoots and downregulated in roots by S. meliloti infection. The differences in expression responses to S. meliloti infection between roots and shoots were in agreement with the results of free indoleacetic acid (IAA) content measurements. The identification and expression analysis of MtGH3 genes at the early phase of S. meliloti infection may help us to understand the role of GH3-mediated IAA homeostasis in the regulation of nodule formation in model legumes M. truncatula.

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Acknowledgments

This work was funded by the National Natural Science Foundation of China (31401935) and Zhejiang Provincial Natural Science Foundation of China under Grant No. LQ14C060001, LQ14C020002 and LQ13H28006.

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The authors declare that they have no competing interests.

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Authors and Affiliations

  1. College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China

    Yanjun Yang, Tao Sun, Houqing Zeng, Huizhong Wang & Chenjia Shen

  2. Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China

    Runqing Yue

  3. Department of Plant Pathology, Washington State University, Pullman, WA, 99164-6430, USA

    Lei Zhang

  4. College of Life Sciences, Zhejiang University, Hangzhou, 310058, China

    Wei Chen

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  1. Yanjun Yang
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Correspondence to Chenjia Shen.

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Yanjun Yang and Runqing Yue contributed equally to this work.

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Yang, Y., Yue, R., Sun, T. et al. Genome-wide identification, expression analysis of GH3 family genes in Medicago truncatula under stress-related hormones and Sinorhizobium meliloti infection. Appl Microbiol Biotechnol 99, 841–854 (2015). https://doi.org/10.1007/s00253-014-6311-5

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