Abstract
Propionic acid is currently produced mainly via petrochemicals, but there is increasing interest in its fermentative production from renewable biomass. However, the current propionic acid fermentation process suffers from low product yield and productivity. In this work, the gene encoding phosphoenolpyruvate carboxylase (PPC) was cloned from Escherichia coli and expressed in Propionibacterium freudenreichii. PPC catalyzes the conversion of phosphoenolpyruvate to oxaloacetate with the fixation of one CO2. Its expression in P. freudenreichii showed profound effects on propionic acid fermentation. Compared to the wild type, the mutant expressing the ppc gene grew significantly faster, consumed more glycerol, and produced propionate to a higher final titer at a faster rate. The mutant also produced significantly more propionate from glucose under elevated CO2 partial pressure. These effects could be attributed to increased CO2 fixation and resulting changes in the flux distributions in the dicarboxylic acid pathway.
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Acknowledgments
This work was supported in part by The Dow Chemical Company. We thank Prof. Murooka of Osaka University, Japan, for providing the plasmid pKHEM04 used in this work. The Egyptian government’s scholarship (General Mission) to Ammar is also acknowledged.
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Ammar, E.M., Jin, Y., Wang, Z. et al. Metabolic engineering of Propionibacterium freudenreichii: effect of expressing phosphoenolpyruvate carboxylase on propionic acid production. Appl Microbiol Biotechnol 98, 7761–7772 (2014). https://doi.org/10.1007/s00253-014-5836-y
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DOI: https://doi.org/10.1007/s00253-014-5836-y