Abstract
The human KIR genes encode a family of class I MHC receptors that are expressed on subsets of NK cells. The expression of KIR proteins is controlled by a stochastic process, and competition between sense and antisense promoter elements has been suggested to program the variegated expression of these genes. Previous studies have demonstrated distinct roles of distal, intermediate, and proximal sense promoter/enhancer elements in gene activation and expression. Conversely, proximal and intronic antisense promoter transcripts have been associated with gene silencing at different stages of NK cell development. In the current study, we examine the effect of intermediate promoter deletion on KIR2DL1 expression in the YTS cell line. Homozygous deletion of the KIR2DL1 intermediate element did not affect proximal promoter activity but resulted in increased detection of upstream transcripts. No significant changes in alternative mRNA splicing or expression levels of KIR2DL1 protein were observed. However, intermediate element deletion was associated with a reduced frequency of gene activation by 5-azacytidine. Taken together, these results indicate that the intermediate element is not an enhancer required for KIR expression; however, it is required for the efficient activation of the gene.
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Introduction
Natural killer (NK) cells are lymphoid cells of the innate immune system that can eradicate stressed cells, such as virus-infected and tumor cells, without prior sensitization (Hamerman et al. 2005). The cytolytic activity of NK cells is controlled by receptors that interrogate potential target cells for either aberrant or missing major histocompatibility complex (MHC) class I molecules on their surfaces (Yokoyama 1998; Parham and Guethlein 2018). The predominant category of MHC class I receptors expressed by human NK cells is derived from the killer cell immunoglobulin-like receptor (KIR) family of genes (Yawata et al. 2002). Fourteen KIR genes and two pseudogenes have been identified and mapped to human chromosome 19q13.4 (Trowsdale et al. 2001). Furthermore, the KIR gene cluster has been shown to exhibit a remarkable diversity with respect to gene content and alleles (Middleton and Gonzelez 2010). The human KIR proteins are a family of type I transmembrane glycoproteins that are expressed probabilistically by mature NK cells and a small percentage of T cells (Moretta et al. 1990; Valiante et al. 1997; Yawata et al. 2008). The KIR family of MHC class I receptors possesses a repertoire of both inhibitory and activating receptors which distinguish between normal host MHC class I expression versus missing or modified class I molecules that arise during neoplasia and viral pathogenesis. The net balance of reciprocity between these inhibitory and activating signals ultimately determines the fate of the target cell (Purdy and Campbell 2009).
KIR gene expression has been shown to be variegated, resulting in NK cells with various combinations of specific KIR genes, with most expressing between 1–3 KIR (Yawata et al. 2008; Li et al. 2008). The mechanisms which regulate the probabilistic expression of KIR genes are not fully understood. However, evidence suggests that interaction of sense RNA transcripts originating from distal promoters with antisense transcripts from the bidirectional KIR proximal promoter or an intronic antisense promoter may play a significant role (Fig. 1; Anderson 2014; Pascal et al. 2006; Stulberg et al. 2007; Davies et al. 2007). The KIR loci are initially silenced in stem cells by a noncoding antisense transcript originating within intron 2 that splices into the first exon, producing an antisense transcript covering the promoter region and ending at a polyA site ~ 300 bp upstream of exon 1. MZF1 was identified as a key transcription factor driving the stem cell-specific activity of the intron 2 antisense promoter (Fig. 1A; Wright et al. 2013). Thus, no KIR transcription can be detected in CD34 hematopoietic progenitors (Fig. 1B). Non-translatable distal KIR transcripts first appear in NK precursors potentially activating the downstream bidirectional proximal KIR promoter and subsequentially competing/interacting with antisense transcripts to determine whether the gene is silenced or remains in a state capable of protein expression. The KIR distal promoter contains a MYC-binding site, and overexpression of MYC in developing NK cells has been shown to augment both transcription from this promoter and the frequency of NK cells that express KIR (Fig. 1C, D; Cichocki et al. 2009). Antisense transcripts from the proximal KIR promoter are ~ 300 bp, unspliced, and polyadenylated and can be detected in developing NK cells up to the CD56+ dim/KIR− stage. The antisense lncRNAs have been shown to silence the KIR proximal promoter through the formation of a 28 bp Piwi RNA (piRNA) which mediates gene silencing (Fig. 1A, D, E; Cichocki et al. 2010). An additional intermediate promoter region located ~ 250 bp upstream of the proximal promoter was identified and shown to produce translatable mRNA transcripts (Fig. 1E, F; Wright et al. 2014). Once KIR protein expression is initiated, antisense transcripts are no longer detectable in CD56+ KIR+ cells (Fig. 1F).
KIR2DL4 is a unique member of this gene family, as it is not expressed in a variegated manner, and all mature CD56+ NK cells express KIR2DL4 (Rajagopalan and Long 1999). The KIR2DL4 gene contains two distal promoters 900 bp and 11 kb upstream of the bidirectional proximal promoter (Stulberg et al. 2007). Transcripts originating from both distal promoters are spliced to 190 bp upstream of the start codon. The distal location of KIR2DL4 promoters prevents the formation of promoter-associated dsRNA from the proximal reverse transcripts and subsequent gene silencing (Stulberg et al. 2007). Therefore, studies conducted thus far suggest that sense transcripts originating from both distal and proximal KIR promoters are essential for gene expression while the presence of antisense transcription is indicative of gene silencing.
A study of individuals with low KIR2DL1 expression led to the identification of a novel KIR2DL1 allele (KIR2DL1*0040115) with a cluster of three single nucleotide polymorphisms (SNPs) in the distal promoter region (Wright et al. 2014). Analysis of the three SNPs revealed that they create a ZEB1-binding site near the MYC/USF-binding site that was associated with a significant reduction in distal transcription. Paradoxically, proximal KIR2DL1 transcripts from these individuals increased, but surface expression of KIR2DL1 was diminished, and a direct correlation of expression with levels of distal transcript was observed. Transcripts from the KIR2DL1*0040115 allele showed increased inclusion of alternative exons with stop codons. The KIR2DL1 distal element may act as an enhancer, as suggested by our earlier findings which demonstrated that individuals with KIR2DL1 alleles containing ZEB1 SNPs exhibited decreased transcription from both distal and intermediate promoters. To address the hypothesis that the translatable intermediate KIR2DL1 transcripts are the primary driver of protein expression, a 281 bp region containing the intermediate promoter region was deleted in YTS cells using CRISPR. In contrast to the ZEB1 mutation, deletion of the intermediate promoter region did not decrease distal transcript levels; however, it did result in decreased efficiency of KIR2DL1 gene activation without decreased proximal transcription.
Methods
NK cell isolation
Healthy volunteers were recruited through the NCI-Frederick Research Donor Program (http://ncifrederick.cancer.gov/programs/science/rdp/default.aspx). The KIR genotype of each donor was determined as previously described (Martin and Carrington 2008). Donors possessing only the KIR2DL1*003 allele were chosen to match the allele present in the YTS cell line. NK cells were separated from the peripheral blood of healthy donors by Histopaque (Sigma-Aldrich) gradient centrifugation using the RosetteSep Human NK Cell Enrichment Cocktail (STEMCELL Technologies).
Cell culture
YTS cells were cultured in RPMI 1640 medium supplemented with 10% FBS (Hyclone), 100 units/ml penicillin, 100 µg/ml streptomycin, and 2 mM L-glutamine (Gibco) at 37 °C and 5% CO2. Cells were passaged every 3–4 days and plated at 3 × 105 cells/ml in 75 cm2 flasks. The KIR+ cell line was derived by treating parental YTS with 5-azacytidine transiently for 72 h followed by washing and staining with anti-KIR2DL1 antibody. The KIR2DL1-expressing cells were purified by three rounds of FACS sorting until a stable line was generated.
CRISPR/Cas 9 deletion of the KIR2DL1 intermediate promoter
The following oligonucleotide sequences were used as CRISPR guides to delete a 281 bp region containing the KIR2DL1 intermediate promoter/enhancer region:
3′ Guide-For5′-CACCGTTAGGCATCTCGTGTTCGGG-3′
3′ Guide-Rev5′-AAACCCCGAACACGAGATGCCTAAC-3′
5′ Guide-For5′-CACCGAAACTCCAGAATTTACAGGT-3′
5′ Guide-Rev5′-AAACACCTGTAAATTCTGGAGTTTC-3′
Bold bases indicate sequence added for cloning into vectors. The 3′ guide was inserted into pSpCas9(BB)-2A-GFP (PX458) and the 5′ guide was inserted into pU6-(BbsI)-CBh-Cas9-T2A-mCherry, both obtained from Addgene. Plasmids (1 µg each) were digested with BbsI (New England Biolabs) for 30 min at 37 °C. The digested plasmids were purified using ChargeSwitch PCR Clean-Up Kit (Invitrogen). Each pair of target sequence oligos (10 µM final) was phosphorylated with T4 Polynucleotide Kinase (New England Biolabs) at 37 °C for 30 min, denatured at 95 °C for 5 min, and then slowly annealed down to 25 °C at 5 °C per minute in a thermocycler. Phosphorylated and annealed target sequence oligos were diluted 1:200 and ligated into BbsI digested plasmids with T4 DNA Ligase (New England Biolabs). Plasmids were transformed into One Shot™ Top10 Chemically Competent E. coli (Invitrogen) and purified using the ZymoPURE™ II Plasmid Midiprep kit (Zymo Research). 1 × 106 YTS cells were transfected with GFP and mCherry plasmids containing guide oligos using an Amaxa Nucleofector II transfection system. Cells were pelleted at 200 × g and resuspended in 100 µl of Amaxa transfection buffer with 5 µg of both plasmids. Cells were electroporated using program X-005 and subsequently were cultured for 48 h prior to single-cell sorting of GFP and mCherry double-positive clones into 96 well plates.
Single-cell sorting for GFP/mCherry+ cells
KIR2DL1 intermediate promoter edit clones were sorted using either a Symphony S6 or FACS Aria-II cell sorter (BD Biosciences) to deposit single GFP/mCherry double-positive cells into individual wells of 96 well plates. YTS cells that had been transfected were counted, washed twice, resuspended in sorting buffer (1 × PBS pH7.4, 1% BSA, 0.5 mM EDTA, and 25 mM HEPES), and passed through 30-μm mesh (CellTrics, Sysmex) prior to sorting to remove doublets and aggregated cells. Stringent signal pulse width gates were applied by the sorting software for further assurance of clonality. Sorted single cells were collected in 96 well plates filled with 100 µl/well of RPMI 1640 supplemented with 10% FBS and glutamine/pen/strep. Plates were monitored for growth, and clones were subsequently expanded.
Screening of YTS intermediate promoter edit clones
Genomic DNA was isolated from the YTS intermediate edit clones with the Quick-DNA™ Mini Prep kit (Zymo Research). Thirty nanograms of DNA from each clone was screened by PCR using 2DL1 distal-For (5′-GGAGAGAGACAGACGGAAAAC-3′) and 2DL1 intermediate-Rev (5′-CGCTAGAATTTGACACCTAGTG-3′) primers to identify clones with homozygous or heterozygous deletions and unedited (wild-type) clones.
Cell transfection and luciferase reporter assay
5 × 105 YTS cells were seeded per well in a 24-well plate the day before transfection; 4.5 µg of reporter construct DNA, 400 ng of Renilla DNA, and 6 µl of jetOPTIMUS transfection reagent (Genesee Scientific) were diluted in 150 µl buffer and incubated at room temperature for 10 min before addition to each well. The DNA mixture containing plasmid DNA was then added to each well and incubated at 37 °C in 5% CO2 for 48 h before luciferase activity analysis. Luciferase activity was assayed using the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions. Briefly, the culture medium was removed 48 h post-transfection, and cells were washed with 0.5 ml of phosphate-buffered saline (PBS, pH 7.4). Cells were then lysed with passive lysis buffer. The suspensions were centrifuged at 14,000 rpm for 1 min. A total of 20 μl of cell lysate supernatant was mixed with 100 μl of luciferase substrate, and the light units were measured on a luminometer. Measurements of firefly luciferase activity from promoter constructs were normalized relative to the activity of the Renilla luciferase produced by the pRLSV40 control vector, and each construct was tested in duplicate in at least three independent experiments.
RNA isolation
Total RNA was isolated from YTS cells using the RNeasy® Mini Kit (Qiagen). Cells were lysed in buffer RLT per manufacturer’s recommendations, and lysates were loaded on RNeasy Mini Spin Columns and washed with buffer RW1 and spun by centrifugation at 10,000 × g for 1 min. On-column DNase I digestion was performed using the RNase-Free DNase Set (Qiagen). Digested samples were washed with buffer RW1 followed by two washes with buffer RPE spinning by centrifugation at 10,000 × g after each wash. RNA samples were eluted in 25 µl of elution buffer, and RNA concentrations were determined by using a NanoDrop 2000 Spectrophotometer (Fisher Scientific).
Poly(A)+ mRNA was isolated using the NEBNext® High Input Poly(A) mRNA Isolation Module (New England Biolabs). Fifty micrograms of purified total RNA was the input for each poly(A)+ mRNA sample in a 200 µl reaction. Poly(A)+ mRNA was captured and washed on oligo(T)25 paramagnetic beads provided by the manufacturer, eluted with 17 µl of Tris buffer, and quantified using a NanoDrop 2000 Spectrophotometer (Fisher Scientific). Typical yields ranged from 400 to 600 ng of poly(A)+ mRNA per sample.
cDNA synthesis
cDNA samples were synthesized using TaqMan® Reverse Transcription Reagents (Applied Biosystems). Each cDNA synthesis reaction was performed in a 20 µl volume with either 1 µg of total RNA or 100 ng of poly(A)+ RNA, 1.75 mM MgCl2, 2 mM dNTPs mix, 2.5 µM random hexamers, 1 U RNase Inhibitor, and 1 U of MultiScribe™ RT. The cDNA was synthesized using a 2720 Thermal Cycler (Applied Biosystems) with a single cycle of 25 °C for 10 min, 37 °C for 30 min, and 95 °C for 5 min, followed by a 4 °C hold.
PCR of KIR2DL1 transcripts
PCR amplifications of KIR transcripts were performed in 40 µl reactions with ZymoTaq premix. Each PCR reaction utilized 2 µl of cDNA template, 1 µM of forward and reverse primers, and 20 µl of 2X ZymoTaq premix. Initial denaturation was performed at 95 °C for 10 min per the manufacturer’s recommendation, 30–35 cycles of 95 °C for 30 s, 30 s annealing at a primer-specific temperature, and extension at 72 °C for 0.5–1.5 min for transcripts up to 1 kb in length. A final extension was performed at 72 °C for 7 min.
PCR primers
2DL1 exon 1-For5′-CGGCAGCACCATGTCGCTCT-3′
2DL1 exon 3-Rev5′-AGGTCCCTGCCAGGTCTTGCG-3′
2DL1 intermediate-For5′GAGTTGGTCATAGTGAAGGACACTAG-3′
2DL1 distal-For5′-GGAGAGAGACAGACGGAAAAC-3′
B-actin-For5′-CCTGGCACCCAGCACAATG-3′
B-actin-Rev5′-GGGCCGGACTCGTCATACT-3′
KIR2DL1 detection by flow cytometry
1 × 106 YTS cells were washed twice in staining buffer (1 × PBS pH 7.4 without Ca/Mg, 1% BSA, 0.5 mM EDTA, and 0.1% NaN3 sodium azide) by centrifugation at 100 × g for 10 min in 5-ml polystyrene round-bottom tubes (Falcon 352,052). YTS cell pellets were incubated with 1 µg PE-conjugated CD158/KIR2DL1 antibody (clone REA284, Miltenyi Biotec) for 30 min on ice, washed twice with staining buffer, gently resuspended in Cytofix™ buffer (4.2% formaldehyde, BD Biosciences), and then stored at 4 °C until analysis. Data was collected using a FACS Symphony A5 cytometer (BD Biosciences) and analyzed with FlowJo v10.8.1 (BD). Antibody binding data were expressed as % positive compared to unstained control cells; PE median fluorescence was also assessed to indicate receptor density.
Bulk cell sorting for KIR2DL1+ cells
KIR2DL1+ cells were sorted to uniform high purity using either a Symphony S6 or FACS Aria-II cell sorter (BD Biosciences). YTS cells were counted, washed twice, resuspended in sorting buffer (1 × PBS pH7.4, 1% BSA, 0.5 mM EDTA, and 25 mM HEPES), and incubated with 10 µl PE-conjugated CD158/KIR2DL1 antibody (clone REA284, Miltenyi Biotec) for 30 min on ice. Labeled cells were washed twice with sorting buffer, resuspended in 0.5–15 ml sorting buffer, passed through 30-μm mesh (CellTrics, Sysmex), and maintained at 4 °C during sorting. Sorted KIR2DL1+ cells were collected in RPMI 1640 supplemented with 10% FBS and glutamine/pen/strep. Sorted cells were monitored for growth and subsequently expanded.
Statistical analysis
Mann–Whitney U and two-tailed t-tests were performed using Prism version 9 for Mac OS; p < 0.05 was regarded to be statistically significant.
Results
Deletion of the KIR2DL1 intermediate promoter region
Our previous study of the intermediate promoter regions of the KIR genes revealed distinct sequence elements associated with the major classes of KIR (Li et al. 2016), suggesting that this region may play a role in the tissue specificity or timing of KIR expression. To address the role of this element in KIR expression, CRISPR guides were designed to delete a 281 bp region encompassing the KIR2DL1 intermediate promoter, including the observed transcription start sites. Figure 2A shows a schematic of the KIR2DL1 5′ region, including the observed retention of intron 1 and the alternative exons in intron 2 that contain stop codons. The region of the KIR2DL1 gene targeted for deletion contains previously characterized AP1-, OCT-, and ETS-binding sites (Li et al. 2016), as well as predicted binding sites for the lineage-determining transcription factors NFIL3 and EBP1. Transfection of vectors containing both the Cas9 gene and guide RNAs into the YTS cell line resulted in the efficient deletion of the intermediate promoter element (Fig. 2B). Individual clones were isolated, and the presence of the 281 bp deletion in selected clones was verified by PCR of genomic DNA (Fig. 2C). Sequencing confirmed that the expected deletion was the only alteration in the 2 kb intergenic region. Figure 2D shows the pattern of deletions observed in 6 individual clones. The 5′ cut occurred primarily 3 bp upstream from the end of the guide sequence, and the 3′ cut varied between − 5 to + 2 bp from the end of the guide sequence.
The KIR2DL1 intermediate element does not enhance proximal promoter activity
Luciferase reporter assays were performed in transfected YTS cells to confirm that the intermediate promoter deletion resulted in the loss of promoter activity and to test the effect of the intermediate element on proximal promoter activity. Figure 3 shows the luciferase activity of the intermediate promoter, the combined intermediate and proximal promoters, and the full distal-intermediate-proximal 5′ region. As expected, the 281 bp deletion significantly impacted intermediate promoter activity. However, there was no loss of proximal promoter activity associated with the deletion of the intermediate element, indicating a lack of enhancer activity in the YTS cell line. In the full 5′ region construct, deletion of the intermediate region increased promoter activity, suggesting an inhibitory effect of transcription from this region on either the upstream distal promoter or the downstream proximal promoter. The increased promoter activity of the full-length construct with deletion of the intermediate element did not reach statistical significance due to a large variation in light unit activity between experiments. However, the trend of increased activity in the deleted construct as compared to the wild-type construct was maintained.
Intermediate promoter deletion increases upstream transcripts without affecting alternative splicing
We had previously noted effects of the introduction of an inhibitory ZEB1-binding site on the utilization of alternative exons 2a and 2b which contain premature stop codons that would block KIR expression when included in spliced mRNAs. Decreased distal transcription in KIR2DL1 alleles containing the ZEB1 site was associated with increased proximal transcription and inclusion of alternative exons in peripheral blood NK cells. Figure 4A shows a comparison of KIR2DL1 transcript isoforms in immature CD56bright versus mature CD56dim peripheral blood NK cells from individuals bearing only KIR2DL1*003 alleles. KIR2DL1*003 is expressed by a higher percentage of NK cells than other common KIR2DL1 alleles, and it is the allele present in the YTS cell line (Dunphy et al. 2015). CD56bright NK cells had distal transcripts containing alternative exon 2b, with no detectable productively spliced KIR2DL1 mRNA. Distal transcripts in CD56dim NK cells contained both splice forms, suggesting a switch from untranslatable to productively spliced KIR2DL1 mRNA in mature NK cells. Intermediate transcripts showed primarily productively spliced mRNAs, indicating that exon 2a inclusion is associated with transcription from the distal promoter. Figure 4B, C shows transcripts detected in YTS clones induced to express KIR2DL1 with 5-azacytidine. Consistent with the results observed in Fig. 4A, alternatively spliced KIR2DL1 mRNAs were readily detected in distal transcripts but not intermediate transcripts. Furthermore, the deletion of the intermediate region did not affect alternative splicing, indicating that intermediate transcripts are not alternatively spliced (Fig. 4C). Although alternatively spliced mRNAs containing exons 2a and/or 2b could be readily detected in peripheral blood NK cell RNA or 5-azacytidine-induced YTS cells (Fig. 4A–C), they were not detected in YTS clones stably expressing KIR2DL1 protein, likely due to the low to undetectable levels of distal transcription (Fig. 4D). Interestingly, transcription from the region adjacent to the intermediate element was increased in stable KIR2DL1-expressing YTS clones containing the 281 bp deletion, suggesting that elements in the region flanking the deleted element contain promoter activity that was unmasked by the deletion. The increased promoter activity of the full distal–proximal construct lacking the intermediate promoter region is consistent with this result (Fig. 3). However, the enhanced transcriptional activity is not due to increased distal promoter activity since PCR with a distal primer did not detect significant levels of distal transcripts in clones with the deletion (Fig. 4D).
Intermediate promoter deletion affects the efficiency of gene activation
FACS analysis was performed on YTS clones to determine the effect of intermediate promoter deletion on gene activation and expression. The parental YTS line and 8 subclones from the CRISPR experiment with an intact intermediate region were compared with 9 subclones that had a homozygous deletion of the intermediate element. The 5-azacytidine-induced expression was significantly reduced in deleted clones relative to control clones, with one clone in the unedited group exhibiting low expression (clone I8; Fig. 5A) and one clone in the edited group that had the same level of induction as unedited clones (clone K7; Fig. 5B). These results suggest that the intermediate KIR2DL1 element plays a significant role in the induction of gene expression. To determine if there was an effect of the deletion on the level of protein expression, KIR2DL1-expressing subclones were isolated from 5-azacytidine-induced clones. Three unedited and three edited subclones with stable expression of KIR2DL1 were isolated, and the relative levels of KIR2DL1 expression were determined. As shown in Fig. 5C, only K8 had a significant reduction in median fluorescence intensity, and it showed the least efficient induction of KIR2DL1 expression (Fig. 5B). The unusual behavior of this clone may be related to the unique 3′ deletion junction observed in this clone (Fig. 2D). The reduced expression of K8 is also consistent with an observed decrease in proximal transcripts (Fig. 4B). These results support the predicted role of the intermediate promoter in gene activation rather than acting as an enhancer for the proximal promoter.
Discussion
Previous studies have established a central role for the KIR proximal promoter in the regulation of KIR expression. Methylation of the proximal promoter has been shown to represent the primary mechanism distinguishing silent KIR genes from those that are actively transcribed and expressed, and treatment with the demethylating agent 5-azacytidine is sufficient to induce KIR expression in NK cell lines (Santourlidis et al. 2002; Chan et al. 2003). Notably, unlike silent mouse Ly49 genes which contain both repressive histone signatures and gene methylation (Rouhi et al. 2006; 2007), silent KIR genes are found in regions with active histone signatures and thus seem to be primarily controlled at the level of DNA methylation (Santourlidis et al. 2008). Although the precise mechanism controlling the differential methylation of KIR genes has yet to be fully defined, antisense transcription from either proximal or intronic promoters has been implicated (Cichocki et al. 2010; Wright et al. 2013). Furthermore, distal KIR transcription in immature NK cells and progenitors may play a role in the initial opening of the KIR loci (Fig. 1C; Cichocki et al. 2009). In the current study, we addressed the role of an intermediate promoter/enhancer element in KIR gene activation and expression. Previous analyses revealed gene-specific differences in the properties of the intermediate element among distinct KIR gene subfamilies, such as the KIR2DL2/KIR2DL3 genes that are expressed earlier and at a higher frequency after bone marrow transplantation (Fischer et al. 2007; Li et al. 2016). Additionally, reduced distal/intermediate transcription in the KIR2DL1*0040115 allele was associated with decreased KIR protein expression, even though proximal transcription was increased, suggesting that either distal or intermediate transcripts are required for KIR expression (Wright et al. 2014). In the current study, we observe that deletion of the intermediate element does not decrease the activity of the proximal promoter, making it unlikely that the intermediate region acts as an enhancer element (Fig. 3). Although deletion of the KIR2DL1 intermediate element in luciferase reporter constructs reduced in vitro promoter activity, homozygous deletion of the element in the YTS cell line resulted in an unexpected increase in upstream transcription. It is conceivable that negative regulatory elements are present within the intermediate region. However, the observed increase in upstream transcripts exhibited by edited YTS clones stably expressing KIR2DL1 had no effect on the level of proximal transcripts or KIR protein expression compared to wild-type cells. Deletion of the intermediate element did not alter the production of alternatively spliced transcripts retaining intron 1 or exons 2a/2b, indicating that the production of untranslatable mRNAs is associated with distal rather than intermediate transcription. However, distal transcripts seem to be required, and their level is correlated with the level of proximal transcripts during 5-azacytidine-induced gene activation. While the distal transcript is low/undetectable in stable KIR2DL1-expressing cells, the highest levels of protein expression seen in 5-azacyticine treated cells in this study were observed in both the edit clone K7 and wild-type clone A4. Both clones had higher levels of distal transcript than their respective counterparts and exhibited higher levels of surface KIR2DL1 expression. The most significant effect of intermediate element deletion was on the efficiency of gene activation by 5-azacytidine-induced gene demethylation. This is consistent with our previous observation that the intermediate element is distinct in KIR gene subfamilies with different expression characteristics, suggesting that the principal role of this element is to initiate gene expression.
In conclusion, the current study implicates the intermediate element in the process of KIR gene activation and suggests that the distal element may act as an enhancer and play a role in regulating the level of KIR expression. The study of KIR gene regulation reveals the importance of upstream/downstream promoter elements in the initial opening of the locus, in contrast to the traditional view of a proximal promoter regulated by enhancer/silencer elements. Additional studies examining the effect of deletion of additional upstream and downstream KIR enhancer/promoter elements active at various stages of NK cell development will be required to achieve a complete understanding of KIR gene regulation.
Data Availability
All sequence information is available in public databases. Primary data, recombinant DNA, and cell lines generated in this study are available upon request.
References
Anderson SK (2014) Probabilistic bidirectional promoter switches: noncoding RNA takes control. Mol Ther Nucleic Acids 3:e191. https://doi.org/10.1038/mtna.2014.42
Chan HW, Kurago ZB, Stewart CA, Wilson MJ, Martin MP, Mace BE, Carrington M, Trowsdale J, Lutz CT (2003) DNA methylation maintains allele-specific KIR gene expression in human natural killer cells. J Exp Med 197:245–255. https://doi.org/10.1084/jem.20021127
Cichocki F, Hanson RJ, Lenvik T, Pitt M, McCullar V, Li H, Anderson SK, Miller JS (2009) The transcription factor c-Myc enhances KIR gene transcription through direct binding to an upstream distal promoter element. Blood 113:3245–3253. https://doi.org/10.1182/blood-2008-07-166389
Cichocki F, Lenvik T, Sharma N, Yun G, Anderson SK, Miller JS (2010) Cutting edge: KIR antisense transcripts are processed into a 28-base PIWI-like RNA in human NK cells. J Immunol 185:2009–2012. https://doi.org/10.4049/jimmunol.1000855
Davies GE, Locke SM, Wright PW, Li H, Hanson RJ, Miller JS, Anderson SK (2007) Identification of bidirectional promoters in the human KIR genes. Genes Immun 8:245–253. https://doi.org/10.1038/sj.gene.6364381
Dunphy SE, Guinan KJ, Chorcora CN, Jayaraman J, Traherne JA, Trowsdale J, Pende D, Middleton D, Gardiner CM (2015) 2DL1, 2DL2 and 2DL3 all contribute to KIR phenotype variability on human NK cells. Genes Immun 16:301–310. https://doi.org/10.1038/gene.2015.15
Fischer JC, Ottinger H, Ferencik S, Sribar M, Punzel M, Beelen DW, Schwan MA, Grosse-Wilde H, Wernet P, Uhrberg M (2007) Relevance of C1 and C2 epitopes for hemopoietic stem cell transplantation: role for sequential acquisition of HLA-C-specific inhibitory killer Ig-like receptor. J Immunol 178:3918–3923. https://doi.org/10.4049/jimmunol.178.6.3918
Hamerman JA, Ogasawara K, Lanier LL (2005) NK cells in innate immunity. Curr Opin Immunol 17:29–35. https://doi.org/10.1016/j.coi.2004.11.001
Li H, Pascal V, Martin MP, Carrington M, Anderson SK (2008) Genetic control of variegated KIR gene expression: polymorphisms of the bi-directional KIR3DL1 promoter are associated with distinct frequencies of gene expression. PLoS Genet 4:e1000254. https://doi.org/10.1371/journal.pgen.1000254
Li H, Wright PW, McCullen M, Anderson SK (2016) Characterization of KIR intermediate promoters reveals four promoter types associated with distinct expression patterns of KIR subtypes. Genes Immun 17:66–74. https://doi.org/10.1038/gene.2015.56
Martin MP, Carrington M (2008) KIR locus polymorphisms: genotyping and disease association analysis. Methods Mol Biol 415:49–64. https://doi.org/10.1007/978-1-59745-570-1_3
Middleton D, Gonzelez F (2010) The extensive polymorphism of KIR genes. Immunology 129:8–19. https://doi.org/10.1111/j.1365-2567.2009.03208.x
Moretta A, Bottino C, Pende D, Tripodi G, Tambussi G, Viale O, Orengo A, Barbaresi M, Merli A, Ciccone E (1990) Identification of four subsets of human CD3-CD16+ natural killer (NK) cells by the expression of clonally distributed functional surface molecules: correlation between subset assignment of NK clones and ability to mediate specific alloantigen recognition. J Exp Med 172:1589–1598. https://doi.org/10.1084/jem.172.6.1589
Parham P, Guethlein LA (2018) Genetics of natural killer cells in human health, disease, and survival. Annu Rev Immunol 36:519–548. https://doi.org/10.1146/annurev-immunol-042617-053149
Pascal V, Stulberg MJ, Anderson SK (2006) Regulation of class I major histocompatibility complex receptor expression in natural killer cells: one promoter is not enough! Immunol Rev 214:9–21. https://doi.org/10.1111/j.1600-065X.2006.00452.x
Purdy AK, Campbell KS (2009) Natural killer cells and cancer: regulation by the killer cell Ig-like receptors (KIR). Cancer Biol Ther 8(23):2211–2220. https://doi.org/10.4161/cbt.8.23.10455
Rajagopalan S, Long EO (1999) A human histocompatibility leukocyte antigen (HLA)-G-specific receptor expressed on all natural killer cells. J Exp Med 189:1093–1100. https://doi.org/10.1084/jem.189.7.1093
Rouhi A, Brooks CG, Takei F, Mager DL (2007) Plasticity of Ly49g expression is due to epigenetics. Mol Immunol 44:821–826. https://doi.org/10.1016/j.molimm.2006.04.006
Rouhi A, Gagnier L, Takei F, Mager DL (2006) Evidence for epigenetic maintenance of Ly49a monoallelic gene expression. J Immunol 176:2991–2999. https://doi.org/10.4049/jimmunol.176.5.2991
Santourlidis S, Graffmann N, Christ J, Uhrberg M (2008) Lineage-specific transition of histone signatures in the killer cell Ig-like receptor locus from hematopoietic progenitor to NK cells. J Immunol 180:418–425. https://doi.org/10.4049/jimmunol.180.1.418
Santourlidis S, Trompeter HI, Weinhold S, Eisermann B, Meyer KL, Wernet P, Uhrberg M (2002) Crucial role of DNA methylation in determination of clonally distributed killer cell Ig-like receptor expression patterns in NK cells. J Immunol 169:4253–4261. https://doi.org/10.4049/jimmunol.169.8.4253
Stulberg MJ, Wright PW, Dang H, Hanson RJ, Miller JS, Anderson SK (2007) Identification of distal KIR promoters and transcripts. Genes Immun 8:124–130. https://doi.org/10.1038/sj.gene.6364363
Trowsdale J, Barten R, Haude A, Stewart CA, Beck S, Wilson MJ (2001) The genomic context of natural killer receptor extended gene families. Immunol Rev 181:20–38. https://doi.org/10.1034/j.1600-065x.2001.1810102.x
Valiante NM, Uhrberg M, Shilling HG, Lienert-Weidenbach K, Arnett KL, D’Andrea A, Phillips JH, Lanier LL, Parham P (1997) Functionally and structurally distinct NK cell receptor repertoires in the peripheral blood of two human donors. Immunity 7:739–751. https://doi.org/10.1016/s1074-7613(00)80393-3
Wright PW, Huehn A, Cichocki F, Li H, Sharma N, Dang H, Lenvik TR, Woll P, Kaufman D, Miller JS, Anderson SK (2013) Identification of a KIR antisense lncRNA expressed by progenitor cells. Genes Immun 14:427–433. https://doi.org/10.1038/gene.2013.36
Wright PW, Li H, Huehn A, O’Connor GM, Cooley S, Miller JS, Anderson SK (2014) Characterization of a weakly expressed KIR2DL1 variant reveals a novel upstream promoter that controls KIR expression. Genes Immun 15:440–448. https://doi.org/10.1038/gene.2014.34
Yawata M, Yawata N, Abi-Rached L, Parham P (2002) Variation within the human killer cell immunoglobulin-like receptor (KIR) gene family. Crit Rev Immunol 22:463–482
Yawata M, Yawata N, Draghi M, Partheniou F, Little AM, Parham P (2008) MHC class I-specific inhibitory receptors and their ligands structure diverse human NK-cell repertoires toward a balance of missing self-response. Blood 112:2369–2380. https://doi.org/10.1182/blood-2008-03-143727
Yokoyama WM (1998) Natural killer cell receptors. Curr Opin Immunol 10:298–305. https://doi.org/10.1016/s0952-7915(98)80168-4
Funding
This project has been funded in whole or in part with federal funds from the Frederick National Laboratory for Cancer Research, National Institutes of Health, under contract HHSN261200800001E. This research was supported in part by the Intramural Research Program of NIH, Frederick National Lab, Center for Cancer Research.
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Conceptualization: S. K. A. Methodology: P. W. W., H. L., M. A. R., and S. K. A. Formal analysis and investigation: P. W. W., H. L., M. A. R., E. M. A., M. K., J. C., and S. K. A. Writing—original draft preparation: P. W. W. and S. K. A. Writing—review and editing: P. W. W., H. L., M. A. R., E. M. A., J.C., and S. K. A. Supervision: S. K. A.
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Wright, P.W., Li, H., Rahman, M.A. et al. The KIR2DL1 intermediate upstream element participates in gene activation. Immunogenetics 75, 495–506 (2023). https://doi.org/10.1007/s00251-023-01321-9
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DOI: https://doi.org/10.1007/s00251-023-01321-9