Abstract
Knowledge of human leukocyte antigen (HLA) allele frequencies is essential for bone marrow and kidney donor searches. The Romanian Caucasian population is heterogeneous and information on HLA polymorphism has not been well studied. We characterized the HLA genetic profile and allele frequencies of regional populations in Romania. HLA-A, B and DRB1 alleles were examined in 8252 individuals, belonging to the four main regions of Romania. The most common alleles found in the Romanian population are the following: HLA-A*01, A*02, A*03, A*11, A*24; HLA-B*18, B*35, B*44, B*51 and HLA-DRB1*01, DRB1*03, DRB1*07, DRB1*11, DRB1*13, DRB1*15, DRB1*16. More than half of the alleles are non-homogeneously spread in Romania. These results provide a starting point for future analyses of genetic heterogeneity in Romania.
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Ahn SH, Han KH, Park JY, Lee CK, Kang SW, Chon CY, Kim YS, Park K, Kim DK, Moon YM (2000) Association between hepatitis B virus infection and HLA-DR type in Korea. Hepatology 31:1371–1373
Cuzick J, Terry G, Ho L, Monaghan J, Lopes A, Clarkson P, Duncan I (2000) Association between high-risk HPV types, HLA DRB1* and DQB1* alleles and cervical cancer in British women. Br J Cancer 82:1348–1352
Erlich HA, Opelz G, Hansen (2001) HLA DNA typing and transplantation. Immunity 14:347–356
Godkin A, Davenport M, Hill AV (2005) Molecular analysis of HLA class II associations with hepatitis B virus clearance and vaccine nonresponsiveness. Hepatology 41:1383–1390
Guan W, Liang L, Boehnke M, Abecasis GR (2009) Genotype-based matching to correct for population stratification in large-scale case–control genetic association studies. Genet Epidemiol 33:508–517
Kim J, Mueller CW (1978a) Factor analysis: what it is and how to do it. Sage Publications, Beverly Hills
Kim J, Mueller CW (1978b) Factor analysis: statistical methods and practical issues. Sage Publications, Beverly Hills
Klein J, Sato A (2000) The HLA system. First of two parts. N Engl J Med 343:702–709
Mack SJ, Bugawan TL, Moonsamy PV, Erlich JA, Trachtenberg EA, Paik YK, Begovich AB, Saha N, Beck HP, Stoneking M, Erlich HA (2000) Evolution of Pacific/Asian populations inferred from HLA class II allele frequency distributions. Tissue Antigens 55:383–400
Mack SJ, Sanchez-Mazas A, Single RM, Meyer D, Hill J, Dron HA, Jani AJ, Thomson G, Erlich HA (2007) Population samples and genotyping technology. Tissue Antigens 69:188–191
Mahmud SM, Robinson K, Richardson H, Tellier PP, Ferenczy AS, Roger M, Coutlée F, Franco EL (2007) HLA polymorphisms and cervical human papillomavirus infection in a cohort of Montreal University students. J Infect Dis 196:82–90
Marsh SGE, Parham P, Barber LD (2000) Evolution and anthropology of HLA. In: Marsh SGE (ed) The HLA facts book. Academic, London, pp 73–78
Metcalfe S, Roger M, Faucher MC, Coutlée F, Franco EL, Brassard P (2013) The frequency of HLA alleles in a population of Inuit women of northern Quebec. Int J Circumpolar Health 72:21350
Middleton D (2014) New allele frequency database: http://www.allelefrequencies.net
Ramezani A, Hasanjani Roshan MR, Kalantar E, Eslamifar A, Banifazl M, Taeb J, Aghakhani A, Gachkar L, Velayati AA (2008) Association of human leukocyte antigen polymorphism with outcomes of hepatitis B virus infection. J Gastroenterol Hepatol 23:1716–1721
Reed E, Ho E, Lupu F, McManus P, Vasilescu R, Foca-Rodi A, Suciu-Foca N (1992) Polymorphism of HLA in the Romanian population. Tissue Antigens 39:8–13
Sanchez-Mazas A, Vidan-Jeras B, Nunes JM, Fischer G, Little AM, Bekmane U, Buhler S, Buus S, Claas FHJ, Dormoy A, Dubois V, Eglite E, Eliaou JF, Gonzalez-Galarza F, Grubic Z, Ivanova M, Lie B, Ligeiro D, Lokki ML, Martins da Silva B, Martorell J, Mendonça D, Middleton D, Papioannou Voniatis D, Papasteriades C, Poli F, Riccio ME, Spyropoulou Vlachou M, Sulcebe G, Tonks S, Toungouz Nevessignsky M, Vangenot C, Walraven AM, Tiercy JM (2012) Strategies to work with HLA data in human populations for histocompatibility, clinical transplantation, epidemiology and population genetics: HLA-NET methodological recommendations. Int J Immunogenet 39:459–476
Scotto G, Fazio V, D’Alessandro G, Monno L, Saracino A, Palumbo E, Angarano G (2003) Association between HLA class II antigens and hepatitis C virus infection. J Biol Regul Homeost Agents 17:316–321
Wang K (2009) Testing for genetic association in the presence of population stratification in genome-wide association studies. Genet Epidemiol 33:637–645
Yuliwulandari R, Kashiwase K, Nakajima H, Uddin J, Susmiarsih TP, Sofro ASM, Tokunaga K (2009) Polymorphisms of HLA genes in Western Javanese (Indonesia): close affinities to Southeast Asian populations. Tissue Antigens 73:46–53
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The authors are grateful to Biosupply Company for the technical support which made this study possible.
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Constantinescu, I., Boșcaiu, V., Cianga, P. et al. The frequency of HLA alleles in the Romanian population. Immunogenetics 68, 167–178 (2016). https://doi.org/10.1007/s00251-015-0891-9
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DOI: https://doi.org/10.1007/s00251-015-0891-9