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The Combined Effect of Temperature and Host Clonal Line on the Microbiota of a Planktonic Crustacean

  • Host Microbe Interactions
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Abstract

Host-associated microbiota vary across host individuals and environmental conditions, but the relative importance of their genetic background versus their environment is difficult to disentangle. We sought to experimentally determine the factors shaping the microbiota of the planktonic Crustacean, Daphnia magna. We used clonal lines from a wide geographic distribution, which had been kept under standardized conditions for over 75 generations. Replicate populations were kept for three generations at 20 and 28 °C. The interaction of the host clonal line and environment (i.e., temperature) influenced microbiota community characteristics, including structure, the relative abundance of common microbial species, and the microbial richness and phylogenetic diversity. We did not find any correlation between host-associated microbiota and the geographic origin of the clones or their temperature tolerance. Our results highlight the prominent effects that host clonal lineage and its interaction with the environment has on host-associated microbiota composition.

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Acknowledgements

We thank Jürgen Hottinger, Elodie Burcklen, Marilou Sison-Mangus, Mahendra Mariadassou, Lev Yampolsky, and members of the Ebert Group at the Zoological Institute for assistance in the laboratory and helpful discussion and feedback. We also thank the anonymous reviewers for their comments on the manuscript.

Funding

This work was supported with grants from the European Research Council, the Swiss National Science, and the Forschungsfonds of the University of Basel.

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  1. Samuel Pichon

    Present address: Institut de Recherche sur la Biologie de l’Insecte, UMR CNRS 7261, bâtiment I étage 1 bureau 1340, Université François Rabelais de Tours, Parc Grandmont, 37200, Tours, France

  2. Karen E. Sullam and Samuel Pichon contributed equally to this work.

Authors and Affiliations

  1. Zoological Institute, University of Basel, Vesalgasse 1, 4051, Basel, Switzerland

    Karen E. Sullam, Samuel Pichon, Tobias M. M. Schaer & Dieter Ebert

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  1. Karen E. Sullam
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Correspondence to Karen E. Sullam.

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Sullam, K.E., Pichon, S., Schaer, T.M.M. et al. The Combined Effect of Temperature and Host Clonal Line on the Microbiota of a Planktonic Crustacean. Microb Ecol 76, 506–517 (2018). https://doi.org/10.1007/s00248-017-1126-4

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