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Copper Content and Resistance Mechanisms in the Terrestrial Moss Ptychostomum capillare: A Case Study in an Abandoned Copper Mine in Central Spain

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Abstract

We present a case study on the tissue absorption of copper of a widely distributed moss species, Ptychostomum capillare in the polluted soil of an abandoned copper mine in central Spain. We studied the soil properties in a copper soil pollution gradient and sampled the moss tufts growing on them in four plots with contrasted soil copper levels. We determined the copper content in the soil and in the moss tissues. On these moss samples, we also performed histochemical tests and X-ray dispersive spectrometry coupled with scanning electron microscopy (SEM-EDX), both in untreated shoots and in samples where surface waxes were removed. We checked the behavior of this species using a metallophillous moss, Scopelophila cataractae, for comparative purposes. Copper contents in P. capillare seem to depend more on available, rather than total soil copper contents. Our results indicate that this moss is able to concentrate 12-fold the available soil copper in soil with low available copper content, whereas in the most polluted soil the concentration of Cu in the moss was only half those levels. Both histochemical and SEM-EDX tests show no surface copper in the mosses from the least polluted plot, whereas in samples from the soil with highest copper content, the removal of surface waxes also reduces or removes copper from the moss shoots. Our observations point at a mixed strategy in P. capillare in this copper mine, with metal accumulation behavior in the lowest Cu plot, and an exclusion mechanism involving wax-like substances acting as a barrier in the most polluted plots. These distortions impede the estimation of environmental levels and thus compromise the value of this moss in biomonitoring. We highlight the need of extending these studies to other moss species, especially those used in biomonitoring programs.

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Acknowledgements

The authors want to thank Esperanza Salvador and Enrique Rodríguez (SEM-EDX Laboratory, SIdI, UAM) and Dr. Carlos García-Delgado (AAS Laboratory, Department of Agricultural Chemistry, UAM) for their helpfulness and kind assistance. They also thank three anonymous reviewers for their careful revision and their insights, which have helped significantly to improve the manuscript.

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Authors and Affiliations

  1. Terrestrial Ecology at Ecological and Forestry Applications Research Centre (CREAF- UAB), Barcelona, Spain

    N. J. Elvira

  2. CIBC-UAM, Dpto. Biología, Fac. Ciencias, Universidad Autónoma de Madrid, Madrid, Spain

    N. G. Medina

  3. Department of Biogeography and Global Change, Museo Nacional de Ciencias Naturales (MNCN-CSIC), Dpto. Biología, Fac. Ciencias, Universidad Autónoma de Madrid, Madrid, Spain

    M. Leo

  4. Dpto. Geología y Geoquímica, Fac. Ciencias, Universidad Autónoma de Madrid, Madrid, Spain

    V. Cala

  5. Dpto. Biología, Fac. Ciencias, Universidad Autónoma de Madrid, Madrid, Spain

    B. Estébanez

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  1. N. J. Elvira
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Elvira, N.J., Medina, N.G., Leo, M. et al. Copper Content and Resistance Mechanisms in the Terrestrial Moss Ptychostomum capillare: A Case Study in an Abandoned Copper Mine in Central Spain. Arch Environ Contam Toxicol 79, 49–59 (2020). https://doi.org/10.1007/s00244-020-00739-6

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