Abstract
Exosomes are special extracellular vesicles secreted by cells, which are of great significance in the basic research of life science and clinical application and has become a hot research field with rapid development in recent 10 years. Therefore, the isolation and separation of exosomes is particularly important for the research and application of exosomes. This paper aims to review the research progress of exosome isolation and separation methods in recent years, including ultracentrifugation, ultrafiltration, size‑exclusion chromatography, precipitation, immunomagnetic bead capture method, aptamer-based isolation, and isolation methods based on microfluidic technology. It is generally accepted that most of the existing methods have limitations, for example, ultracentrifugation is time-consuming and laborious, and immunomagnetic bead capture method and aptamer-based separation method have small sample processing capacity and high cost. As a result, we also introduce some common situations in which two or more methods are combined for use. Finally, the separation and isolation methods including all those presented in this review were compared and summarized.
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Acknowledgements
The authors thank the laboratory and the research group for their consistent research on new methods for exosome separation. The authors also thank the foundation for its support.
Funding
This work was supported by National Natural Science Foundation of China international (Regional) Cooperation and exchange project “Research on Bio-inspired New Material Additive Manufacturing (Number 5181101987) “.
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ES performed the conceptualization; WX, JC, LA wrote the main manuscript text; ES was in charge of review and supervise. WX prepared Fig. 1 and Table 1 and was responsible for the edit. All authors have reviewed and approved the final manuscript.
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Xu, WM., Li, A., Chen, JJ. et al. Research Development on Exosome Separation Technology. J Membrane Biol 256, 25–34 (2023). https://doi.org/10.1007/s00232-022-00260-y
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DOI: https://doi.org/10.1007/s00232-022-00260-y