Abstract
The present work was aimed at developing stability enhanced silica gel-supported macroporous chitosan membrane for immobilization of enzymes. The membrane was surface modified using various cross-linking agents for covalent immobilization of enzyme Bovine serum albumin. The results of FT-IR, UV–vis, and SEM analyses revealed the effect of cross-linking agents and confirmed the formation of modified membranes. The presence of silica gel as a support could provide a large surface area, and therefore, the enzyme could be immobilized only on the surface, and thus minimized the diffusion limitation problem. The resultant enzyme immobilized membranes were also characterized based on their activity retention, immobilization efficiency, and stability aspects. The immobilization process increased the activity of immobilized enzyme even higher than that of total (actual) activity of native enzyme. Thus, the obtained macroporous chitosan membranes in this study could act as a versatile host for various guest molecules.
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Acknowledgments
We thank the National Central University and National Science Council (NSC, (Grant No.: NSC100-2923-E-008-001-MY3), Taiwan, Republic of China (ROC) and Department of Science and Technology (DST, Ref no/S/FST/ESI-101/2010), New Delhi, India, for financial support.
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Yang, WY., Thirumavalavan, M., Malini, M. et al. Development of Silica Gel-Supported Modified Macroporous Chitosan Membranes for Enzyme Immobilization and Their Characterization Analyses. J Membrane Biol 247, 549–559 (2014). https://doi.org/10.1007/s00232-014-9671-y
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DOI: https://doi.org/10.1007/s00232-014-9671-y