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Mesenchymal stem cells with modafinil (gold nanoparticles) significantly improves neurological deficits in rats after middle cerebral artery occlusion

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Abstract

Systemic treatments for ischemic stroke as a disease with high disability and death have been yet unsuccessful. Combined treatments can potentially cause better results in treatment of patients with Stroke. In this study we assessed the neuroprotective effect of modafinil-coated gold nanoparticles (AuNPs) and mesenchymal stem cell (MSC) in ischemic stroke rats. Stem cells and AuNPs offer great promise for new medical treatments. 60 male Wistar rats were randomly divided into five groups (12 in each group): (1) the group that developed middle cerebral artery occlusion (MCAO or ischemia), (2) the normal group (control), (3) the MCAO group that received MSC (C + MCAO), (4) the MCAO group that received MSC and modafinil (CM + MCAO), and (5) the MCAO group that received MSC and modafinil-coated AuNPs (CMN + MCAO). Middle Cerebral Artery Occlusion (MCAO) was performed by inserting a silicone coat filament in the right internal carotid artery via the external carotid artery until it reached the anterior cerebral artery. The filament was located in the internal carotid artery for 60 min and then removed. Modafinil-coated AuNPs (100 mg/kg) or Modafinil (100 mg/kg) were given to the rats as an oral gavage, once a day in the morning time. Finally, infarct volume, BDNF (Brain-derived neurotrophic factor), GDNF (Glial cell-derived neurotrophic factor), NeuN (neuronal nuclear protein) expression, and cell apoptosis in brain were analyzed. The brain infarct volume and apoptosis significantly decreased and BDNF, NeuN, and GDNF increased in C + MCAO, CM + MCAO, and CMN + MCAO groups compared to ischemia. CMN + MCAO groups did not show significant difference in these factors compared to control group. These results demonstrated that the administration of stem cells and Modafinil-coated AuNPs at the same time had a good effect on ischemic brain injuries. It happened through increasing neurotrophic factors and decreasing brain cell apoptosis.

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Authors and Affiliations

  1. Department of Pharmacology, Karaj Branch, Islamic Azad University, Karaj, Iran

    Sepideh Nazarian, Zohreh Abdolmaleki & Alireza Torfeh

  2. Department of Clinical Sciences, Karaj Branch, Islamic Azad University, Karaj, Iran

    Seyed Hamed Shirazi Beheshtiha

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  1. Sepideh Nazarian
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  2. Zohreh Abdolmaleki
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  3. Alireza Torfeh
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  4. Seyed Hamed Shirazi Beheshtiha
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Correspondence to Zohreh Abdolmaleki.

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Communicated by Sreedharan Sajikumar.

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Nazarian, S., Abdolmaleki, Z., Torfeh, A. et al. Mesenchymal stem cells with modafinil (gold nanoparticles) significantly improves neurological deficits in rats after middle cerebral artery occlusion. Exp Brain Res 238, 2589–2601 (2020). https://doi.org/10.1007/s00221-020-05913-9

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  • DOI: https://doi.org/10.1007/s00221-020-05913-9

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