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Comprehensive analysis of phenylpropanoids and butylidenephthalides in Angelica sinensis (Oliv.) Diels from different regions and processing methods by high-performance liquid chromatography combined with chemometrics

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Abstract

Angelicae sinensis radix is commonly used in Chinese medicine and is also widely used as a spice and condiment in many countries. Due to the different geographical sources and processing methods, the contents of chemical components are different and affect the quality of Angelica sinensis radix. A simple and efficient method was established to comprehensively evaluate the quality of Angelica sinensis radix by combining HPLC fingerprint and single-standard multi-component quantitative analysis. With ferulic acid as a single marker, the contents of eight main active ingredients, including ferulic acid, senkyunolide I, senkyunolide H, coniferyl ferulate, E-ligustilide, E-butylidenephthalide, Z-ligustilide, and Z-butylidenephthalide, were simultaneously determined in 30 min by reliable relative correction factor. The overall chemical profiles of Angelica sinensis radix were compared by fingerprint similarity evaluation, the specific content level of each component was compared by simultaneous content determination of multiple components of a single standard, and the specific different components of Angelica sinensis radix before and after processing were analyzed by chemometrics. The established method was successfully applied to the quality evaluation of 44 batches of Angelica sinensis radix from 4 different origins and 45 batches of Angelica sinensis radix with different processing methods. This study proposed a simple, rapid, low-cost, and reliable quality comprehensive evaluation method for ASR from different origins and processing methods, which may be readily transferred and applied to other industries.

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Data availability

The data that support the findings of this study are available from the authors, upon reasonable request.

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Acknowledgements

This work was supported by Organizational Key Research and Development Program of Shanghai University of Traditional Chinese Medicine [Number 2023YZZ02]; Sanming Project of Medicine in Shenzhen (grant number SZZYSM202106004); National Key R&D Program of China under Grant [Number 2018YFC1707900 and 2018YFC1707001]; Research Project of Science and Technology Commission of Shanghai Municipality under Grant [Number 19DZ2200400].

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Author notes

  1. Yan Sun, Jing Zhou and Yuxin Hu made equal contributions to this work.

Authors and Affiliations

  1. School of Pharmacy, Henan University, Kaifeng, 475004, China

    Yan Sun & Wanying Wu

  2. Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China

    Yan Sun, Jing Zhou, Yuxin Hu, Sihan Chen, Hui Ni, Ruihong Feng, Zijia Zhang, Min Lei, Jinjun Hou, Huali Long, Wanying Wu & De-an Guo

  3. School of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing, 210029, China

    Yuxin Hu, Hui Ni & Wanying Wu

  4. University of Chinese Academy of Sciences, Beijing, 100049, China

    Sihan Chen, Wanying Wu & De-an Guo

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  1. Yan Sun
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Correspondence to Huali Long or Wanying Wu.

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The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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Supplementary Information

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Supplementary file 1: Table S1.

The information of 89 batches of medicinal materials, raw decoction pieces and wine-processed Angelicae Sinensis Radix samples. Table S2. The parameters of the optimization for sample preparation. Table S3. The content (%) of the eight analytes with different extraction solvents. Table S4. The stability (%) of the eight analytes in 34 hours. Table S5. The system suitability parameters of different columns. Table S6. The information about the three chromatographic systems. Table S7. The RRT of each component in three chromatographic systems

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Sun, Y., Zhou, J., Hu, Y. et al. Comprehensive analysis of phenylpropanoids and butylidenephthalides in Angelica sinensis (Oliv.) Diels from different regions and processing methods by high-performance liquid chromatography combined with chemometrics. Eur Food Res Technol 250, 983–998 (2024). https://doi.org/10.1007/s00217-023-04422-7

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