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Screening of Lactobacillus strains that enhance SCFA uptake in intestinal epithelial cells

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Abstract

The objective was to screen Lactobacillus strains with strong survivability in oro-gastrointestinal tract and adhesion abilities of intestinal mucins and Caco-2 cells, and their effect on absorption of short-chain fatty acids (SCFAs) by epithelial cells was studied. The survival rate of Lactobacillus strains was studied after exposure to oral stress, gastric stress and intestinal stress successively, and then their adhesion ability was also researched. The model of intestinal epithelial cells absorbing SCFAs was established, which was used to evaluate the effect of Lactobacillus strains on Caco-2 cells absorption of SCFAs. The survival rate of Lactobacillus plantarum L58, L67, L97, L123, and L198 and Lactobacillus fermentum L146 was significantly higher than others in oro-gastrointestinal tract (P < 0.05), which also showed high adhesion to mucins and Caco-2 cells. The model was successfully established with the Caco-2 cell line, which formed a polarized cell monolayer and developed tight junctions with an appropriate permeability coefficient for phenol red lower than 1 × 10–6 cm/s after culturing for 15 days, and the viability of Caco-2 cells was significantly higher than other concentrations when the content of propionic acid or butyric acid was 1 mmol/L in the model. The propionic acid content in Caco-2 cells inoculated with L. plantarum L58, L67, L97, L123, and L198 was significantly higher than that of cells without L. plantarum inoculation (P < 0.05), and the butyric acid content in cells inoculated with L. fermentum L146 was significantly higher than that of cells without inoculation (P < 0.05). Our results highlight that L. plantarum L58, L67, L97, L123, L198 and L. fermentum L146 are more resistant to oro-gastrointestinal conditions and their high adhesion to the intestine can enhance SCFAs uptake in intestinal epithelial cells.

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Abbreviations

SCFAs:

Short-chain fatty acids

GC–MS:

Gas chromatography-mass spectrometer

OGT:

Oro-gastrointestinal

MRS:

De Man, Rogosa and Sharpe

PBS:

Phosphate buffered saline

CFDA-SE:

5-(And 6-) carboxyfluorescein diacetate, succinimidyl ester

MEM:

Modified Eagle's medium

FBS:

Fetal bovine serum

NEAA:

Nonessential amino acids

PSN:

Penicillin/streptomycin/neomycin

Ap:

Apical side

BL:

Basolateral

TEER:

Trans-epithelial electrical resistance

P app :

Apparent permeability coefficients

TEM:

Transmission electron microscopy

CCK-8:

Cell counting kit-8 reagent

RSD:

Relative standard deviation

QC:

Quality control

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Acknowledgements

This study was financially supported by the National Natural Science Foundation of China (31701627, 31972094), the Natural Science Foundation of the Jiangsu Higher Education Institutions of China National (19KJA140004, 17KJB550009), the National Key Research and Development Program of China (2019YFF0217602) and the Major Science and Technology Application Demonstration Program of Chengdu (2019-YF09-00055-SN).

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Authors and Affiliations

  1. College of Food Science and Engineering, Yangzhou University, Yangzhou, People’s Republic of China

    Da-wei Chen, Chun-meng Chen, Heng-xian Qu, Chen-yu Ren, Xian-tao Yan, Yu-jun Huang, Cheng-ran Guan, Chen-chen Zhang & Rui-xia Gu

  2. Jiangsu Key Laboratory of Dairy Biotechnology and Safety Control, Yangzhou, People’s Republic of China

    Da-wei Chen, Chun-meng Chen, Heng-xian Qu, Chen-yu Ren, Xian-tao Yan, Yu-jun Huang, Cheng-ran Guan, Chen-chen Zhang & Rui-xia Gu

  3. Jiangsu Dairy Biotechnology Engineering Research Center, Kang Yuan Dairy Co. Ltd., Yangzhou University, Yangzhou, People’s Republic of China

    Da-wei Chen, Chun-meng Chen, Heng-xian Qu, Chen-yu Ren, Xian-tao Yan, Yu-jun Huang, Cheng-ran Guan, Chen-chen Zhang & Rui-xia Gu

  4. New Hope Dairy Co., Ltd, Chengdu, People’s Republic of China

    Qi-ming Li

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  1. Da-wei Chen
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Da-wei Chen, Chun-meng Chen, Heng-xian Qu, Chen-yu Ren, Xian-tao Yan, Yu-jun Huang, Cheng-ran Guan, Chen-chen Zhang, Qi-ming Li and Rui-xia Gu declare that they have no conflict of interest.

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Chen, Dw., Chen, Cm., Qu, Hx. et al. Screening of Lactobacillus strains that enhance SCFA uptake in intestinal epithelial cells. Eur Food Res Technol 247, 1049–1060 (2021). https://doi.org/10.1007/s00217-021-03686-1

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