Abstract
SIRT1 is the homologue of SIR2 that links to longevity in lower organisms and has a direct or indirect role that positively regulates the metabolic pathway via insulin signaling. RSV, a naturally occurring small polyphenol that activates SIRT1, has been reported to ameliorate the early development of metabolic syndrome of high-fat-diet-fed mice at a low dose (0.005 w/w) in 10 weeks through inhibition of fatty acid synthase and other relevant enzymes. However, what effect of a higher dose of dietary RSV on the long-term high-fat-diet-fed C57BL/6J mice has not been reported in view of regulating SIRT1 and related genes. This study investigated the effect of a higher dose of RSV (0.1 % w/w) on glucose and lipid metabolism in 13-week high-fat-diet-fed C57BL/6J mice as well as on mRNA and protein expression regulations with respect to relevant genes. The data showed that a higher dose of RSV supplementation significantly lowered the concentration of glucose, plasma total cholesterol, total triglyceride concentrations, and hepatic cholesterol in high-fat-diet-fed mice. Relative mRNA expressions of SIRT1, FOXO1, PPARγ, and PGC-1α were significantly up-regulated, and corresponding protein expressions were also enhanced.
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Abbreviations
- RSV:
-
Resveratrol (3, 5, 4′-trihydroxystilbene)
- SIRT1:
-
Sirtuin 1
- SIR2:
-
Silent information regulator 2
- FOXO1:
-
Forkhead box O1
- PPARγ:
-
Peroxisome proliferator-activated receptor γ
- PGC-1α:
-
Peroxisome proliferator-activated receptor γ coactivator-1alpha
- HDL-C:
-
High-density lipoprotein cholesterol
- total-C:
-
Total cholesterol
- TG:
-
Triglycerides
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Acknowledgments
This work was supported by grants from the National Natural Science Foundation of China (Grant No. 81072521), Tianjin University of Science and Technology (Grant No. 20100411). and Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi (STIP).
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The mice were all treated in strict accordance with the Tianjin University of Science & Technology guidelines for the care and use of laboratory animals.
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Yu, Y., Zhang, N., Jiang, H. et al. Resveratrol treatment improves plasma and blood glucose concentration and lipid metabolism in high-fat-fed C57BL/6J mice. Eur Food Res Technol 242, 1849–1856 (2016). https://doi.org/10.1007/s00217-016-2684-2
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DOI: https://doi.org/10.1007/s00217-016-2684-2