Abstract
The present study was designed to investigate the antioxidant properties [selected polyphenol content (SPC), radical scavenging activity] and colour intensity of Greek unifloral honeys. For this purpose, one hundred and seventy honey samples of different botanical origin (thyme, pine, orange blossom, and fir) were collected during the harvesting period 2011 and 2012 from thirteen different regions in Greece. The phenolic profile was evaluated based on the quantification of syringic acid, myricetin, quercetin, kaempferol, and chrysin using high performance liquid chromatography. Radical scavenging activity and colour intensity were estimated using spectrometric assays. Honey samples analysed showed variations in the SPC (3.86 ± 0.67 to 65.79 ± 8.08 mg/kg), colour intensity (164 ± 49 to 517 ± 110 mAU) and % radical scavenging activity (% RSA) (17.24 ± 5.50 to 50.38 ± 19.17), depending on botanical origin. On the basis of results obtained, the tested honey samples may be considered as easily accessible natural sources of antioxidants and valuable supplements to the daily diet.
Similar content being viewed by others
References
Erdman Jr JW, Balentine D, Arab L, et al. (2007) Flavonoids and heart health. In: Proceedings of the ILSI North America Flavonoids Workshop, May 31–June 1 2005, Washington, DC. J Nutr 137(3):718–737
Gheldof N, Wang XH, Engeseth NJ (2002) Identification and quantification of antioxidant components of honeys from various floral sources. J Agric Food Chem 50:5870–5877
Wang XH, Gheldof N, Engeseth NJ (2004) Effect of processing and storage on antioxidant capacity of honey. J Food Sci 69:96–101
Turkmen N, Sari F, Poyrazoglu ES, Velioglu YS (2006) Effects of prolonged heating on antioxidant activity and colour of honey. Food Chem 95:653–657
Bertoncelj J, Dobersek U, Jamnik M, Golob T (2007) Evaluation of the phenolic content, antioxidant activity and colour of Slovenian honey. Food Chem 105:822–828
Schramm DD, Karim M, Schrader HR, Holt RR, Cardetti M, Keen C (2003) Honey with high levels of antioxidants can provide protection to healthy human subjects. J Agric Food Chem 51(6):1732–1735
Al-Waili NS (2003) Identification of nitric oxide metabolites in various honeys: effects of intravenous honey on plasma and urinary nitric oxide metabolites concentrations. J Med Food 6(4):359–364
Al-Mamary M, Al-Meeri A, Al-Habori M (2002) Antioxidant activities and total phenolics of different types of honey. Nutr Res 22(9):1041–1047
Tsiapara AV, Jaakkola M, Chinou I, Graikou K, Tolonen T, Virtanen V, Moutsatsou P (2009) Bioactivity of Greek honey extracts on breast cancer (MCF-7), prostate cancer (PC-3) and endometrial cancer (Ishikawa) cells: profile analysis of extracts. Food Chem 116:702–708
Kassim M, Achoui M, Mohd-Rais M, Ali-Mohd M, Kamaruddin MY (2010) Ellagic acid, phenolic acids, and flavonoids in Malaysian honey extracts demonstrate in vitro anti-inflammatory activity. Nutr Res 30:650–659
Spilioti E, Jaakkola M, Tolonen T, Lipponen M, Virtanen V, Chinou I, Kassi E, Karabournioti S, Moutsatsou P (2014) Phenolic acid composition, antiatherogenic and anticancer potential of honeys derived from various regions in Greece. PLoS One 9(4):e94860
Anklam E (1998) A review of the analytical methods to determine the geographical and botanical origin of honey. Food Chem 63:549–562
Latorre MJ, Peña R, García S, Herrero C (2000) Authentication of Galician (N.W. Spain) honeys by multivariate techniques based on metal content data. Analyst 125:307–312
Popek S (2000) A procedure to identify a honey type. Food Chem 79:401–406
Alissandrakis E, Tarantilis PA, Pappas C, Harizanis PC, Polissiou M (2009) Ultrasound-assisted extraction gas chromatography-mass spectrometry analysis of volatile compounds in unifloral thyme honey from Greece. Eur Food Res Technol 229:365–373
Antony SM, Han IY, Rieck JR, Dawson PL (2000) Antioxidative effect of Maillard reaction products formed from honey at different reaction times. J Agric Food Chem 48:3985–3989
Beretta G, Granata P, Ferrero M, Orioli M, Facino RM (2005) Standardization of antioxidant properties of honey by a combination of spectrophotometric/fluorimetric assays and chemometrics. Anal Chim Acta 533:185–191
Blasa M, Candiracci M, Accorsi A, Piacentini MP, Albertini MC, Piatt E (2006) Raw millefiori honey is packed full of antioxidants. Food Chem 97:217–222
Gorjanović SZ, Alvarez-Suarez JMA, Novaković MM, Pastor FT, Pezo L, Battino M, Sužnjević DZ (2013) Comparative analysis of antioxidant activity of honey of different floral sources using recently developed polarographic and various spectrophotometric assay. J Food Comp Anal 30:13–18
Karabagias ΙΚ, Vavoura MV, Badeka A, Kontakos S, Kontominas MG (2014) Differentiation of Greek thyme honeys according to geographical origin based on the combination of phenolic compounds and conventional quality parameters using chemometrics. Food Anal Method 7:2113–2121
Council Directive 2001/110/EC relating to honey. Official Journal of the European Communities, L 10, 47–52
IHC Working Group” Authenticity of Bee Products”. Summary Report of the annual meeting, 1 October 2013 (during the 43th Apimondia Conference in Kiev, Ukraine)
Sant’Ana LDO, Sousa JPLM, Salgueiro FB, Lorenzon MCA, Castro RN (2012) Characterization of monofloral honeys with multivariate analysis of their chemical profile and antioxidant activity. J Food Sci 71(1):135–140
SPPS, v.22.0 (2013) IBM
Sergiel I, Pohl P, Biesaga M (2014) Characterisation of honeys according to their content of phenolic compounds using high performance liquid chromatography/tandem mass spectrometry. Food Chem 145:404–408
Escriche I, Kadar M, Juan-Borrás M, Domenech E (2014) Suitability of antioxidant capacity, flavonoids and phenolic acids for floral authentication of honey. Impact of industrial thermal treatment. Food Chem 142:135–143
Castro RM, Escamilla MJ, Reig FB (1992) Evaluation of the color of some unifloral honey types as a characterization parameter. J AOAC Int 75:537–542
González-Miret ML, Terrab A, Hernanz D, Fernández-Recamales MA, Heredia FJ (2005) Multivariate correlation between color and mineral composition of honeys and by their botanical origin. J Agric Food Chem 53:2574–2580
Vela L, de Lorenzo C, Pérez RA (2007) Antioxidant capacity of Spanish honeys and its correlation with polyphenol content and other physicochemical properties. J Sci Food Agric 87:1069–1075
Saxena S, Gautam S, Sharma A (2010) Physical, biochemical and antioxidant properties of some Indian honeys. Food Chem 118:391–397
Khalil MI, Mahaneem M, Jamalullail SMS, Alam N, Sulaiman SA (2011) Evaluation of radical scavenging activity and colour intensity of nine Malaysian honeys of different origin. J Apiprod ApiMed Sci 3(1):4–11
Khalil MdI, Moniruzzaman M, Boukraâ L, Benhanifia MdM, Islam A, Islam MdN, Sulaiman SA, Hua Gan S (2012) Physicochemical and antioxidant properties of Algerian honey. Molecules 17:11199–11215
Baltrušaitytė V, Venskutonis PR, Čeksterytė V (2007) Radical scavenging activity of different floral origin honey and beebread phenolic extracts. Food Chem 101:502–514
Estevinho L, Pereira AP, Moreira L, Dias LG, Pereira E (2008) Antioxidant and antimicrobial effects of phenolic compounds extracts of Northeast Portugal honey. Food Chem Toxicol 46:3774–3779
Bogdanov S, Jurendic T, Sieber R, Gallmann P (2008) Honey for nutrition and health: a review. J Am Coll Nutr 27:677–689
Acknowledgments
The authors are grateful to Attiki honey S.A, Athens, Greece, Dr. Sofia Karabournioti and to local beekeepers from Lakonia, for the donation of honey samples. We thank Associate Professor K.A. Riganakos for the donation of pure syringic acid.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Compliance with ethics requirements
This article does not contain any studies with human or animal subjects.
Rights and permissions
About this article
Cite this article
Karabagias, I.K., Dimitriou, E., Kontakos, S. et al. Phenolic profile, colour intensity, and radical scavenging activity of Greek unifloral honeys. Eur Food Res Technol 242, 1201–1210 (2016). https://doi.org/10.1007/s00217-015-2624-6
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00217-015-2624-6