Abstract
Balb/c mice fed 0 mg, 20 mg of β-carotene (BC) or 50 mg of vitamin E (Vit E) per Kg of diet for 7 weeks were immunized intraperitoneally with β-Lactoglobulin (β-Lg). Specific IgG and IgE anti β-Lg were determined in the mouse sera by an enzyme-linked immunosorbent assay (ELISA), and the jejunum was used for assessment of local anaphylactic responses ex vivo in Ussing chambers. Short-circuit current (Isc), a measure of active ion transport, and epithelial conductance (G), a measure of passive ion permeability, were measured for each tissue. Specimens were also processed for examination by histological analysis. Compared with sensitized mice (unsupplemented mice), those fed BC or Vit E had lower β-Lg-specific IgG (p < 0.001 and p < 0.0001, respectively) and IgE levels (p < 0.02 and p < 0.01, respectively). A secretory response reflecting local intestinal anaphylaxis was evident in sensitized mice, as indicated by an increase in Isc (p < 0.009). Sensitized mice demonstrated also an enhanced epithelial permeability as measured by conductance (p < 0.03). BC supplementation resulted in a significant decrease in secretory response (p < 0.02) with no effect on the epithelial permeability. Interestingly, the secretory response and epithelial permeability were significantly reduced by supplementation with Vit E (p < 0.02 and p < 0.009, respectively). These findings provide evidence for the first time that both BC and Vit E regulate epithelial ion transport, but it should be noted that only Vit E-supplemented mice showed a decreased tissue conductance, indicating protection from β-Lg-induced epithelial permeability increase. Analysis of histological sections revealed that BC or Vit E treatment clearly reduced the microscopic lesions caused by β-Lg sensitization.
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Acknowledgments
This research was supported by the Directorate General for Scientific Research and Technological Development (DGRSDT, MESRS, Algeria). The authors would like to thank Mr. Thierno Aliou Diallo for accepting to read our article.
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Compliance with Ethics Requirements
The experiments described in this study comply with the current Algerian legislation covering the protection of animals.
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Grar, H., Dib, W., El Mecherfi, K.E. et al. Supplementation with β-carotene or vitamin E protects against increase in anaphylactic response in β-lactoglobulin-sensitized Balb/c mice: ex vivo study. Eur Food Res Technol 241, 393–398 (2015). https://doi.org/10.1007/s00217-015-2471-5
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DOI: https://doi.org/10.1007/s00217-015-2471-5