Skip to main content
SpringerLink
Log in

Seeds recovered from by-products of selected fruit processing as a rich source of tocochromanols: RP-HPLC/FLD and RP-UPLC-ESI/MSn study

  • Original Paper
  • Published:
European Food Research and Technology Aims and scope Submit manuscript

Abstract

Tocochromanol composition was analysed in the seeds of nine various fruits: apple (Malus domestica Borkh.), red currant (Ribes rubrum L.), gooseberry (Ribes uva-crispa L.), grape (Vitis vinifera L.), pomegranate (Punica granatum L.), Japanese quince (Chaenomeles japonica (Thunb.) Lindl. ex Spach), sea buckthorn (Hippophae rhamnoides L.), watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai) and canary melon (Cucumis melo L.), recovered from by-products of fruit industry. The concentration range of subsequent tocopherols (Ts) and tocotrienols (T3s) was as follows: 1.29–29.30, 0.00–11.68, 0.42–95.11, 0.00–7.66, 0.03–5.03, 0.00–0.18 and 0.00–6.68 mg/100 g of seed dry weight for α-T, β-T, γ-T, δ-T, α-T3, β-T3 and γ-T3, respectively. The δ-T3 was not detected in any of the tested seeds. All tocochromanols were successfully separated by rapid RP-HPLC/FLD method and confirmed by the RP-UPLC-ESI/MS3 technique.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

Abbreviations

RP-HPLC/FLD:

Reverse-phase high-performance liquid chromatograph/fluorescence detector

RP-UPLC-ESI/MSn :

Reverse-phase ultra-performance liquid chromatography–electrospray ionisation/mass spectrometry

T:

Tocopherol

T3:

Tocotrienol

References

  1. FAOSTAT (2013) FAO Statistical Database. http://www.fao.org. Accessed 12 Dec 2013

  2. Górnaś P, Siger A, Juhņeviča K, Lācis G, Šnē E, Segliņa D (2014) Cold‐pressed Japanese quince (Chaenomeles japonica (Thunb.) Lindl. ex Spach) seed oil as a rich source of α‐tocopherol, carotenoids and phenolics: a comparison of the composition and antioxidant activity with nine other plant oils. Eur J Lipid Sci Technol 116:563–570

  3. Górnaś P, Siger A, Pugajeva I, Segliņa D (2014) Sesamin and sesamolin as unexpected contaminants in various cold-pressed plant oils: NP-HPLC/FLD/DAD and RP-UPLC-ESI/MSn study. Food Addit Contam Part A 31:567–573

  4. Górnaś P, Siger A, Segliņa D (2013) Physicochemical characteristics of the cold-pressed Japanese quince seed oil: new promising unconventional bio-oil from by-products for the pharmaceutical and cosmetic industry. Ind Crops Prod 48:178–182

    Google Scholar 

  5. Wie M, Sung J, Choi Y, Kim Y, Jeong HS, Lee J (2009) Tocopherols and tocotrienols in grape seeds from 14 cultivars grown in Korea. Eur J Lipid Sci Technol 111:1255–1258

    CAS  Google Scholar 

  6. Eitenmiller R, Lee J (2004) Vitamin E: food chemistry, composition, and analysis. Marcel Dekker Inc., New York

    Google Scholar 

  7. Dwiecki K, Górnaś P, Jackowiak H, Nogala-Kałucka M, Polewski K (2007) The effect of D-alpha-tocopherol on the solubilization of dipalmitoylphosphatidylcholine membrane by anionic detergent sodium dodecyl sulfate. J Food Lipids 14:50–61

    CAS  Google Scholar 

  8. Dwiecki K, Górnaś P, Wilk A, Nogala-Kałucka M, Polewski K (2007) Spectroscopic studies of D-α-tocopherol concentration-induced transformation in egg phosphatidylcholne vesicles. Cell Mol Biol Lett 12:51–69

    CAS  Google Scholar 

  9. Ruiz RP (2005) Water. In: Wrolstad RE, Decker EA, Schwartz SJ, Sporns P (eds) Handbook of food analytical chemistry, water, proteins, enzymes, lipids, and carbohydrates. Wiley, New Jersey, pp 7–8

    Google Scholar 

  10. Slavin M, Yu LL (2012) A single extraction and HPLC procedure for simultaneous analysis of phytosterols, tocopherols and lutein in soybeans. Food Chem 135:2789–2795

    CAS  Google Scholar 

  11. Górnaś P, Siger A, Czubinski J, Dwiecki K, Segliņa D, Nogala-Kalucka M (2014) An alternative RP‐HPLC method for the separation and determination of tocopherol and tocotrienol homologues as butter authenticity markers: a comparative study between two European countries. Eur J Lipid Sci Technol. doi:10.1002/ejlt.201300319

  12. Górnaś P, Siger A, Polewski K, Pugajeva I, Waśkiewicz A (2014) Factors affecting tocopherol contents in coffee brews: NP-HPLC/FLD, RP-UPLC-ESI/MSn and spectroscopic study. Eur Food Res Technol 238:259–264

    Google Scholar 

  13. Cenkowski S, Yakimishen R, Przybylski R, Muir W (2006) Quality of extracted sea buckthorn seed and pulp oil. Can Agric Eng 48:3.9–3.16

  14. Fromm M, Bayha S, Kammerer DR, Carle R (2012) Identification and quantitation of carotenoids and tocopherols in seed oils recovered from different Rosaceae species. J Agric Food Chem 60:10733–10742

    CAS  Google Scholar 

  15. Habibnia M, Ghavami M, Ansaripourc M, Vosough S (2012) Chemical evaluation of oils extracted from five different varieties of Iranian pomegranate seeds. JFBT 2:35–40

    Google Scholar 

  16. Raziq S, Anwar F, Mahmood Z, Shahid S, Nadeem R (2012) Characterization of seed oils from different varieties of watermelon [Citrullus lanatus (Thunb.)] from Pakistan. Grasas Aceites 63:365–372

    CAS  Google Scholar 

  17. Nyam KL, Tan CP, Lai OM, Long K, Che Man Y (2009) Physicochemical properties and bioactive compounds of selected seed oils. LWT Food Sci Technol 42:1396–1403

    CAS  Google Scholar 

  18. Mariod A, Matthäus B (2008) Fatty acids, tocopherols, sterols, phenolic profiles and oxidative stability of Cucumis melo Var. agrestis oil. J Food Lipids 15:56–67

    CAS  Google Scholar 

  19. Goffman FD, Galletti S (2001) Gamma-linolenic acid and tocopherol contents in the seed oil of 47 accessions from several Ribes species. J Agric Food Chem 49:349–354

    CAS  Google Scholar 

  20. Shintani D, DellaPenna D (1998) Elevating the vitamin E content of plants through metabolic engineering. Science 282:2098–2100

    CAS  Google Scholar 

  21. Fromm M, Bayha S, Carle R, Kammerer DR (2012) Comparison of fatty acid profiles and contents of seed oils recovered from dessert and cider apples and further Rosaceous plants. Eur Food Res Technol 234:1033–1041

    CAS  Google Scholar 

  22. Aggarwal BB, Sundaram C, Prasad S, Kannappan R (2010) Tocotrienols, the vitamin E of the 21st century: its potential against cancer and other chronic diseases. Biochem Pharmacol 80:1613–1631

    CAS  Google Scholar 

  23. Rimm EB, Stampfer MJ, Ascherio A, Giovannucci E, Colditz GA, Willett WC (1993) Vitamin E consumption and the risk of coronary heart disease in men. N Engl J Med 328:1450–1456

    CAS  Google Scholar 

  24. Stampfer MJ, Hennekens CH, Manson JE, Colditz GA, Rosner B, Willett WC (1993) Vitamin E consumption and the risk of coronary disease in women. N Engl J Med 328:1444–1449

    CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by the National Research Programme “Sustainable Use of Local Resources (Entrails of the Earth, Forest, Food and Transport)—New Products and Technologies” project “Improvement and maintenance of apple quality, and new processing possibilities” (2010–2013).

Conflict of interest

None.

Compliance with Ethics Requirements

This article does not contain any studies with human or animal subjects.

Author information

Authors and Affiliations

  1. Latvia State Institute of Fruit-Growing, Graudu 1, Dobele, 3701, Latvia

    Paweł Górnaś & Dalija Segliņa

  2. Institute of Food Safety, Animal Health and Environment BIOR, Lejupes 3, Riga, 1076, Latvia

    Iveta Pugajeva

Authors
  1. Paweł Górnaś
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Iveta Pugajeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dalija Segliņa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Paweł Górnaś.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Górnaś, P., Pugajeva, I. & Segliņa, D. Seeds recovered from by-products of selected fruit processing as a rich source of tocochromanols: RP-HPLC/FLD and RP-UPLC-ESI/MSn study. Eur Food Res Technol 239, 519–524 (2014). https://doi.org/10.1007/s00217-014-2247-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00217-014-2247-3

Keywords

Search

Navigation