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Determination of L-selectin in blood plasma using DNA aptamer–based surface-enhanced Raman spectroscopy assay

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Abstract

In the human body, tumor cell occurrence can be indirectly monitored using the L-selectin concentration in the blood, since selectin ligands are present on the surface of tumor cells, and with tumor progression, a decrease in L-selectin levels can be expected and observed. In this study, we present a selective DNA-based surface-enhanced Raman spectroscopy (SERS) assay for the detection and determination of L-selectin in biological samples. Two calibration curves (linear in the 40–190 ng mL−1 region and exponential in the 40–500 ng mL−1 region) are fitted to the obtained SERS experimental data, i.e., the ratio of I732/I1334 band intensities (LOQ = 46 ng mL−1). Calculated determination coefficients are found to be R2 = 0.997 for the linear region of the calibration curve and R2 = 0.977 for the exponential region. Moreover, we demonstrate very good selectivity of the assay even in the presence of P- and E-selectin in a sample containing L-selectin. With our SERS assay, the L-selectin concentration in biological samples can be estimated directly from the calibration curves.

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Authors and Affiliations

  1. Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224, Warsaw, Poland

    Aneta Aniela Kowalska, Ariadna B. Nowicka, Tomasz Szymborski & Agnieszka Kamińska

  2. Faculty of Materials Engineering and Technical Physics, Poznan University of Technology, Piotrowo 3, 60-965, Poznan, Poland

    Ariadna B. Nowicka

  3. Lukasiewicz Institute of Microelectronics and Photonics, al. Lotnikow 32/46, 02-668, Warsaw, Poland

    Piotr Cywiński

Authors
  1. Aneta Aniela Kowalska
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  2. Ariadna B. Nowicka
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  3. Tomasz Szymborski
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  4. Piotr Cywiński
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  5. Agnieszka Kamińska
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Correspondence to Aneta Aniela Kowalska.

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Ethics approval

Measurement for clinical samples was performed in compliance with the relevant laws and institutional guidelines. The protocol of study was approved by the Ethics and Bioethics Committee of Cardinal Stefan Wyszynski University in Warsaw (Poland): Opinion number 13/2019.

Source of biological material

Plasma samples from patients with tumor were collected according to standard best practice and ethics and bioethics guidelines. Informed consent was obtained from all subjects.

Plasma samples from patients with tumor are gathered by the Department of Pneumonology, Oncology and Allergology, Medical University of Lublin, (Lublin, Poland). Human plasma blood samples from 3 healthy patients (healthy tissue samples) were acquired from Regional Blood Center in Warsaw, Poland.

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The authors declare no competing interests.

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Kowalska, A.A., Nowicka, A.B., Szymborski, T. et al. Determination of L-selectin in blood plasma using DNA aptamer–based surface-enhanced Raman spectroscopy assay. Anal Bioanal Chem 416, 1189–1197 (2024). https://doi.org/10.1007/s00216-023-05110-x

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  • DOI: https://doi.org/10.1007/s00216-023-05110-x

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