Abstract
A novel matrix certified reference material (CRM) for clenbuterol in mutton (GBW 10216) was developed to assist measurement and risk monitoring of clenbuterol in mutton. The candidate CRM raw samples were obtained by oral administration of clenbuterol and investigating the pharmacokinetics of clenbuterol in sheep. A high-precision isotope dilution coupled with liquid chromatography tandem mass spectrometry (LC-ID-MS/MS) method was established and assigned the value of clenbuterol in mutton powder through combined detection of nine inter-laboratories. The certified value with expanded uncertainty was 21.1 ± 2.2 μg/kg (k = 2, 95% confidence) for clenbuterol in mutton. The prepared matrix CRM was sufficiently homogeneous between and within bottles. The long-term stability of clenbuterol in mutton powder was evaluated for 12 months at −20℃ and short-term stability for 7 days at 4℃ and 50℃. The uncertainties originating from characterization, homogeneity, and stability were systematically analyzed and evaluated. The prepared matrix CRM can be applied for proficiency testing and nationwide risk monitoring programs to guarantee the accuracy and comparability of clenbuterol measurement results in mutton.
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Acknowledgements
We sincerely acknowledge Prof. Xiaoou Su and master Meng Liu for providing the candidate CRM mutton samples.
Funding
This research was supported by The National Key Research and Development Program of China (2022YFF0607900), The Ministry of Agriculture and Rural Affairs (MOARA) of the People’s Republic of China (Special Fund for Agro-scientific Research), and Innovation Program of Chinese Academy of Agricultural Sciences.
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All animal experiments were approved by the Animal Care and Ethics Committee of Nanjing Agricultural University (permit number IACECNAU20111105) and followed the Guiding Principles for Biomedical Research Involving Animals.
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Li, F., Zhou, J., Wang, M. et al. Production of a matrix certified reference material for measurement and risk monitoring of clenbuterol in mutton. Anal Bioanal Chem 415, 1487–1496 (2023). https://doi.org/10.1007/s00216-023-04543-8
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DOI: https://doi.org/10.1007/s00216-023-04543-8