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Peptide-anchored biomimetic interface for electrochemical detection of cardiomyocyte-derived extracellular vesicles

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Abstract

Cardiomyocyte-derived extracellular vesicles (EVs) are a promising class of biomarkers that can advance the diagnosis of many kinds of cardiovascular diseases. Herein, we develop a new electrochemical method for the feasible detection of cardiomyocyte-derived EVs in biological fluids. The core design of the method is the fabrication of a peptide-anchored biomimetic interface consisting of a lipid bilayer and peptide probes. On the one hand, the lipid bilayer provides excellent antifouling ability to the electrode interface and facilitates the anchoring of peptide probes. On the other hand, the peptide probes equip the electrode interface with excellent binding capability and affinity to CD172a, a specific marker of cardiomyocyte-derived EVs, thus enabling the efficient and selective detection of target EVs. Taking EVs derived from the heart myoblast cells H9C2 as the model target, the method displays a wide linear detection range from 1 × 103 to 1 × 108 particles/mL with a desirable detection limit of 132 particles/mL. Furthermore, the method shows good performance in biological fluids such as serum, and thus may have great potential for practical use in the diagnosis of cardiovascular diseases.

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All data generated or analyzed during this study are included in this article and its supplementary information file.

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Funding

This work was supported by the grant from Sichuan Medical Association (No. Q17041).

Author information

Authors and Affiliations

  1. Department of Cardiothoracic Surgery, People’s Hospital of Leshan, Leshan, Sichuan, 614000, People’s Republic of China

    Yang Zhou, Fei Zhao, Bo Zheng, Shihai Tang, Juan Gong, Bin He, Zhi Zhang, Na Jiang, Huijuan Zha & Jun Luo

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  1. Yang Zhou
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  2. Fei Zhao
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  3. Bo Zheng
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Contributions

All authors contributed to the study conception and design. The first draft of the manuscript was written by YZ, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Yang Zhou.

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Ethics approval

Clinical experiment was approved by the Ethics Committee of People’s Hospital of Leshan and performed according to ethical standards.

Competing interests

The authors declare no competing interests.

Source of biological material

Serum samples were collected from AMI patients and control donors with informed consent.

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Published in the topical collection Advances in Extracellular Vesicle Analysis with guest editors Lucile Alexandre, Jiashu Sun, Myriam Taverna, and Wenwan Zhong.

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Zhou, Y., Zhao, F., Zheng, B. et al. Peptide-anchored biomimetic interface for electrochemical detection of cardiomyocyte-derived extracellular vesicles. Anal Bioanal Chem 415, 1305–1311 (2023). https://doi.org/10.1007/s00216-022-04419-3

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  • DOI: https://doi.org/10.1007/s00216-022-04419-3

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