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A novel graphene oxide/chitosan foam incorporated with metal–organic framework stationary phase for simultaneous enrichment of glycopeptide and phosphopeptide with high efficiency

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Abstract

A novel hydrophilic porous biocomposite was fabricated by incorporating graphene oxide (GO) @chitosan (CS) foam substrate (GO@CS@ZIF-8 foam) with ZIF-8 crystals in situ via a facile stirring method for simultaneous enrichment of glycopeptides and phosphopeptides from complex biological samples. The experimental results demonstrated that GO@CS@ZIF-8 foam exhibited favorable specificity for simultaneous enrichment of N-glycopeptides and phosphopeptides under the same condition for HRP and β-casein tryptic digest mixtures. The novel material was further applied to enriching both glycopeptides and phosphopeptides simultaneously from 4 μL complex human serum, and 423 N-glycopeptides and 40 phosphopeptides corresponding to 133 glycoproteins and 29 phosphoproteins were identified, respectively.

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Funding

This work was supported by the National Natural Science Foundation of China (Grant Nos. U20A20121 and 61971248), Key Research and Development Program of Zhejiang Province (Grant No. 2020C03064), Science and Technology Major Project of Ningbo (Grant No. 2018B10075), Open Funds of Key Laboratory of Diagnosis and Treatment of Digestive System Tumors of Zhejiang Province (Grant No. KFJJ-202102), and sponsored by the K.C. Wong Magna Fund in Ningbo University (Grant No. xkzwl1507).

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Authors and Affiliations

  1. Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, Institute of Mass Spectrometry, School of Materials Science and Chemical Engineering, Ningbo University, Ningbo, People’s Republic of China

    Rong Liu, Wenqing Gao, Jiaqian Yang, Chenlu Wang & Keqi Tang

  2. Ningbo Institute of Life and Health Industry, University of Chinese Academy of Sciences, Ningbo, People’s Republic of China

    Shun Zhang, Jing Lin & Sijia Zhang

  3. Key Laboratory of Diagnosis and Treatment of Digestive System Tumors of Zhejiang Province, 2019E10020, Ningbo, People’s Republic of China

    Shun Zhang, Jing Lin & Sijia Zhang

  4. Faculty of Electrical Engineering and Computer Science, Ningbo University, Ningbo, People’s Republic of China

    Jiancheng Yu

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  1. Rong Liu
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  2. Wenqing Gao
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  4. Shun Zhang
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Liu, R., Gao, W., Yang, J. et al. A novel graphene oxide/chitosan foam incorporated with metal–organic framework stationary phase for simultaneous enrichment of glycopeptide and phosphopeptide with high efficiency. Anal Bioanal Chem 414, 2251–2263 (2022). https://doi.org/10.1007/s00216-021-03861-z

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