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Simultaneous electrochemical aptasensing of patulin and ochratoxin A in apple juice based on gold nanoparticles decorated black phosphorus nanomaterial

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Abstract

Simultaneous detection of patulin (PAT) and ochratoxin A (OTA) in food products is in great demand, which can prevent toxins from being exposed to human and animal bodies. However, simultaneous detection of multiple targets still faces a challenge. Herein, we developed a novel electrochemical aptasensor for the simultaneous detection of PAT and OTA in apple juice based on gold nanoparticles decorated black phosphorus (AuNPs-BP) nanomaterial. AuNPs-BP function?/work? as a sensing platform for loading much different electrochemical signal molecules functionalized aptamers. In this context, methylene blue functionalized PAT aptamers (Mb-PAT-aptamers) and ferrocene functionalized OTA aptamers (Fc-OTA-aptamers) have been introduced here to fabricate the aptasensor. Fc close to electrode surface showed a strong signal, whereas Mb was far away from electrode surface so exhibited a weak signal in the absence of OTA and PAT. Two kinds of electrochemical signal changes have been recorded dependent on target of OTA and PAT concentrations. So, simultaneous detection of OTA and PAT is achieved. Under the optimum conditions, using this developed biosensor, PAT and OTA can be quantified at a linearity range of 0.01 × 10−7 μg·mL−1 ~ 0.10 μg·mL−1. In addition, it also has good selectivity, stability and repeatability. For the practical application, it shows promising performance for the simultaneous detection of PAT and OTA in apple juice.

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Acknowledgments

The authors gratefully acknowledge financial support from the National Key R&D Program of China (2019YFC1606703), and the Natural Science Foundation of Shaanxi Province in China (2020JM-429).

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  1. Haiyan Zhao and Xiujuan Qiao contributed equally to this work.

Authors and Affiliations

  1. College of Food Science and Technology, Northwest University, Xi’an, 710069, Shaanxi, China

    Xuelian Zhang, Chen Niu, Tianli Yue & Qinglin Sheng

  2. College of Chemistry & Materials Science/Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education/Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, Xi’an, 710069, Shaanxi, China

    Haiyan Zhao & Xiujuan Qiao

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  1. Haiyan Zhao
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Zhao, H., Qiao, X., Zhang, X. et al. Simultaneous electrochemical aptasensing of patulin and ochratoxin A in apple juice based on gold nanoparticles decorated black phosphorus nanomaterial. Anal Bioanal Chem 413, 3131–3140 (2021). https://doi.org/10.1007/s00216-021-03253-3

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