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Silanized carbon dot-based thermo-sensitive molecularly imprinted fluorescent sensor for bovine hemoglobin detection

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Abstract

Using the surface molecular imprinting technique, a thermo-sensitive molecularly imprinted fluorescent sensor was constructed for bovine hemoglobin (BHb) detection with the silanized carbon dots (CD@SiO2) as fluorescent signal, N-isopropylacrylamide as monomer sensitive to temperature, and BHb as template. The silanized carbon dots coated by the molecularly imprinted polymer (CD@SiO2@MIP) were characterized by high-resolution transmission electron microscopy, Fourier transform infrared spectroscopy, and fluorescence spectroscopy. Owing to the combination of the strong fluorescence sensitivity of CDs and the high selectivity of the molecular imprinting shell, the prepared sensor showed good recognition and detection performance to the target protein BHb, with a linear range of 0.31–1.55 μM and a detection limit of 1.55 μM. Furthermore, the sensor was utilized to detect the content of BHb in real urine with a recovery of 98.6–100.5%. The CD@SiO2@MIP sensors present a high potential for applications in the detection of BHb in biological systems.

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References

  1. Duan L, He Q, Yan XH, Cui Y, Wang KW, Li JB. Hemoglobin protein hollow shells fabricated through covalent layer-by-layer technique. Biochem Biophys Res Commun. 2007;354:357–62.

    CAS  PubMed  Google Scholar 

  2. Duan HM, Wang XJ, Wang YH, Li JB, Luo CN. Bioreceptor multi-walled carbon nanotubes@Fe3O4@SiO2-surface molecular imprinted polymer in an ultrasensitive chemiluminescent biosensor for bovine hemoglobin. RSC Adv. 2015;5:88492–9.

    CAS  Google Scholar 

  3. Wang YQ, Zhang HM, Zhou QH. Studies on the interaction of caffeine with bovine hemoglobin. Eur J Med Chem. 2009;44:2100–5.

    CAS  PubMed  Google Scholar 

  4. Chen LX, Xu SF, Li JH. Recent advances in molecular imprinting technology: current status, challenges and highlighted applications. Chem Soc Rev. 2011;40:2922–42.

    CAS  PubMed  Google Scholar 

  5. Chen LX, Wang XY, Lu WH, Wu XQ, Li JH. Molecular imprinting: perspectives and applications. Chem Soc Rev. 2016;45:2137–211.

    CAS  PubMed  Google Scholar 

  6. Wu Q, Li MN, Huang Z, Shao YM, Bai L, Zhou LC. Well-defined nanostructured core-shell magnetic surface imprinted polymers (Fe3O4@SiO2@MIPs) for effective extraction of trace tetrabromobisphenol A from water. J Ind Eng Chem. 2018;60:268–78.

    CAS  Google Scholar 

  7. Gao DM, Zhang ZP, Wu MH, Xie CG, Guan GJ, Wang DP. A surface functional monomer-directing strategy for highly dense imprinting of TNT at surface of silica nanoparticles. J Am Chem Soc. 2007;129:7859–66.

    CAS  PubMed  Google Scholar 

  8. Ge L, Wang SSM, Yu JH, Li NQ, Ge SG, Yan M. Molecularly imprinted polymer grafted porous Au-paper electrode for an microfluidic electro-analytical origami device. Adv Funct Mater. 2013;23:3115–23.

    CAS  Google Scholar 

  9. Ma Y, Pan PGQ, Zhang Y, Guo XZ, Zhang HQ. Narrowly dispersed hydrophilic molecularly imprinted polymer nanoparticles for efficient molecular recognition in real aqueous samples including river water, milk, and bovine serum. Angew Chem Int Ed. 2013;52:1511–4.

    CAS  Google Scholar 

  10. Xu SF, Lu HZ, Li JH, Song XL, Wang AX, Chen LX, et al. Dummy molecularly imprinted polymers-capped CdTe quantum dots for the fluorescent sensing of 2,4,6-trinitrotoluene. ACS Appl Mater Interfaces. 2013;5:8146–54.

    CAS  PubMed  Google Scholar 

  11. Saylan Y, Yilmaz F, Ozgur E, Derazshamshir A, Yavuz H, Denizli A. Molecular imprinting of macromolecules for sensor applications. Sensors. 2017;17:898–928.

    Google Scholar 

  12. Li DY, He XW, Chen Y, Li WY, Zhang YK. Novel hybrid structure silica/CdTe/molecularly imprinted polymer: synthesis, specific recognition, and quantitative fluorescence detection of bovine hemoglobin. ACS Appl Mater Interfaces. 2013;5:12609–16.

    CAS  PubMed  Google Scholar 

  13. Wang XY, Yu SM, Liu W, Fu LW, Wang YQ, Li JH, et al. A molecular imprinting based hybrid ratiometric fluorescence sensor for the visual determination of bovine hemoglobin. ACS Sens. 2018;3:378–85.

    CAS  PubMed  Google Scholar 

  14. Yang YQ, He XW, Wang YZ, Li WY, Zhang YK. Epitope imprinted polymer coating CdTe quantum dots for specific recognition and direct fluorescent quantification of the target protein bovine serum albumin. Biosens Bioelectron. 2014;54:266–72.

    CAS  PubMed  Google Scholar 

  15. Li DY, Zhang XM, Yan YJ, He XW, Li WY, Zhang YK. Thermo-sensitive imprinted polymer embedded carbon dots using epitope approach. Biosens. Bioelectron. 2016;79:187–92.

    CAS  PubMed  Google Scholar 

  16. Zhang Z, Li JH, Wang XY, Shen DZ, Chen LX. Quantum dots based mesoporous structured imprinting microspheres for the sensitive fluorescent detection of phycocyanin. ACS Appl Mater Interfaces. 2015;7:9118–27.

    CAS  PubMed  Google Scholar 

  17. Li W, Sun Y, Yang CC, Yan XM, Guo H, Fu GQ. Fabrication of surface protein-imprinted nanoparticles using a metal chelating monomer via aqueous precipitation polymerization. ACS Appl Mater Interfaces. 2015;7:27188–96.

    CAS  PubMed  Google Scholar 

  18. Bereli N, Andac M, Baydemir G, Say R, Galaev IY, Denizli A. Protein recognition via ion-coordinated molecularly imprinted supermacroporous cryogels. J Chromatogr A. 2008;1190:18–26.

    CAS  PubMed  Google Scholar 

  19. Qin L, He XW, Zhang W, Li WY, Zhang YK. Macroporous thermosensitive imprinted hydrogel for recognition of protein by metal coordinate interaction. Anal Chem. 2009;81:7206–16.

    CAS  PubMed  Google Scholar 

  20. Stobiecka M, Chalupa A. Modulation of plasmon-enhanced resonance energy transfer to gold nanoparticles by protein survivin channeled-shell gating. J Phys Chem B. 2015;119:13227–35.

    CAS  PubMed  Google Scholar 

  21. Stobiecka M. Novel plasmonic field-enhanced nanoassay for trace detection of proteins. Biosens. Bioelectron. 2014;55:379–85.

    CAS  PubMed  Google Scholar 

  22. Li H, Shao FQ, Zou SY, Yang QJ, Huang H, Feng JJ, et al. Microwave-assisted synthesis of N,P-doped carbon dots for fluorescent cell imaging. Microchim. Acta. 2016;183:821–6.

    CAS  Google Scholar 

  23. Wang WP, Lu YC, Huang H, Feng JJ, Chen JR, Wang AJ. Facile synthesis of water-soluble and biocompatible fluorescent nitrogen-doped carbon dots for cell imaging. Analyst. 2014;139:1692–6.

    CAS  PubMed  Google Scholar 

  24. Huang H, Lv JJ, Zhou DL, Bao N. One-pot green synthesis of nitrogen-doped carbon nanoparticles as fluorescent probes for mercury ions. RSC Adv. 2013;3:21691–6.

    CAS  Google Scholar 

  25. Baker SN, Baker GA. Luminescent carbon nanodots: emergent nanolights. Angew Chem Int Ed. 2010;49:6726–44.

    CAS  Google Scholar 

  26. Mao Y, Bao Y, Han DX, Li FH, Niu L. Efficient one-pot synthesis of molecularly imprinted silica nanospheres embedded carbon dots for fluorescent dopamine optosensing. Biosens Bioelectron. 2012;38:55–60.

    CAS  PubMed  Google Scholar 

  27. Wang H, Yi JH, Velado D, Yu YY, Zhou SQ. Immobilization of carbon dots in molecularly imprinted microgels for optical sensing of glucose at physiological pH. ACS Appl. Mater. Interfaces. 2015;7:15735–45.

    CAS  PubMed  Google Scholar 

  28. Zhou Y, Qu ZB, Zeng YB, Zhou TS, Shi GY. A novel composite of graphene quantum dots and molecularly imprinted polymer for fluorescent detection of paranitrophenol. Biosens Bioelectron. 2014;52:317–23.

    CAS  PubMed  Google Scholar 

  29. Liu HC, Ding L, Chen LG, Chen YH, Zhou TY, Li HY, et al. A facile, green synthesis of biomass carbon dots coupled with molecularly imprinted polymers for highly selective detection of oxytetracycline. J Ind Eng Chem. 2019;69:455–63.

    CAS  Google Scholar 

  30. Fang MY, Zhuo KL, Chen YJ, Zhao YJ, Wang JJ. Fluorescent probe based on carbon dots/silica/molecularly imprinted polymer for lysozyme detection and cell imaging. Anal Bioanal Chem. 2019;411:5799–807.

    CAS  PubMed  Google Scholar 

  31. Zhu SJ, Meng QN, Wang L, Zhang JH, Song YB, Jin H, et al. Highly photoluminescent carbon dots for multicolor patterning, sensors, and bioimaging. Angew Chem Int Ed. 2013;52:3953–7.

    CAS  Google Scholar 

  32. Stöber W, Fink A. Controlled growth of monodisperse silica spheres in the micron size range. J Colloid Interface Sci. 1968;26:62–9.

    Google Scholar 

  33. Zhang X, Yang S, Sun LQ, Luo AQ. Surface-imprinted polymer coating L-cysteine-capped ZnS quantum dots for target protein specific recognition. J Mater Sci. 2016;51:6075–85.

    CAS  Google Scholar 

  34. Yang YH, Gao MY. Preparation of fluorescent SiO2 particles with single CdTe nanocrystal cores by the reverse microemulsion method. Adv Mater. 2005;17:2354–7.

    CAS  Google Scholar 

  35. Lutz JF, Akdemir O, Hoth A. Point by point comparison of two thermosensitive polymers exhibiting a similar LCST: is the age of poly (NIPAM) over. J Am Chem Soc. 2006;128:13046–7.

    CAS  PubMed  Google Scholar 

  36. Chen YF, Rosenzweig Z. Luminescent CdS quantum dots as selective ion probes. Anal Chem. 2002;74:5132–8.

    CAS  PubMed  Google Scholar 

  37. Guo T, Deng QL, Fang GZ, Gu DH, Yang YK, Wang S. Upconversion fluorescence metal-organic frameworks thermo-sensitive imprinted polymer for enrichment and sensing protein. Biosens Bioelectron. 2016;79:341–6.

    CAS  PubMed  Google Scholar 

  38. Zhang W, He XW, Li WY, Zhang YK. Thermo-sensitive imprinted polymer coating CdTe quantum dots for target protein specific recognition. Chem Commun. 2012;48:1757–9.

    CAS  Google Scholar 

  39. Wang XY, Yu JL, Li JH, Kang Q, Shen DZ, Chen LX. Quantum dots based imprinting fluorescent nanosensor for the selective and sensitive detection of phycocyanin: a general imprinting strategy toward proteins. Sens Actuat B-Chem. 2018;255:268–74.

    CAS  Google Scholar 

  40. Lv PP, Xie DD, Zhang ZH. Magnetic carbon dots based molecularly imprinted polymers for fluorescent detection of bovine hemoglobin. Talanta. 2018;188:145–51.

    CAS  PubMed  Google Scholar 

  41. Tan L, Kang CC, Xu SY, Tang YW. Selective room temperature phosphorescence sensing of target protein using Mn-doped ZnS QDs-embedded molecularly imprinted polymer. Biosens. Bioelectron. 2013;48:216–23.

    CAS  PubMed  Google Scholar 

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Funding

This study received financial supports from the National Natural Science Foundation of China (Nos. 21873026, 21573058, 21773059, 21303044).

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Authors and Affiliations

  1. Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, 453007, Henan, China

    Yajing Zhao, Yujuan Chen, Mengyao Fang, Yabing Tian, Guangyue Bai & Kelei Zhuo

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  1. Yajing Zhao
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  2. Yujuan Chen
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  3. Mengyao Fang
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  4. Yabing Tian
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  5. Guangyue Bai
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  6. Kelei Zhuo
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Corresponding author

Correspondence to Kelei Zhuo.

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The informed consent was obtained from the healthy volunteer who provide urine sample for this study. All experiments in this study were approved by the Academic Ethics Committee of Henan Normal University, China, and have been performed in accordance with the ethical standards.

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The authors declare that they have no competing interests.

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Zhao, Y., Chen, Y., Fang, M. et al. Silanized carbon dot-based thermo-sensitive molecularly imprinted fluorescent sensor for bovine hemoglobin detection. Anal Bioanal Chem 412, 5811–5817 (2020). https://doi.org/10.1007/s00216-020-02803-5

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  • DOI: https://doi.org/10.1007/s00216-020-02803-5

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