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Recent advances in optical microscopic methods for single-particle tracking in biological samples

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Abstract

With the rapid development of optical microscopic techniques, explorations on the chemical and biological properties of target objects in biological samples at single-molecule/particle level have received great attention recently. In the past decades, various powerful techniques have been developed for single-particle tracking (SPT) in biological samples. In this review, we summarize the commonly used optical microscopic methods for SPT, such as total internal reflection fluorescence microscopy (TIRFM), super-resolution fluorescence microscopy (SRM), dark-field optical microscopy (DFM), total internal reflection scattering microscopy (TIRSM), and differential interference contrast microscopy (DICM). We then discuss the image processing and data analysis methods, including particle localization, trajectory reconstruction, and diffusion behavior analysis. The application of SPT on the cell membrane, within the cell, and the cellular invading process of viruses are introduced. Finally, the challenges and prospects of optical microscopic technologies for SPT are delineated.

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Acknowledgments

This work was supported by national natural science foundation of China (NSFC, Project no. 21522502), the Fundamental Research Funds for the Central Universities, the Excellent Youth Scholars of Hunan Provincial Education Department (17B155) and the Opening Fund of Key Laboratory of Chemical Biology and Tradition Chinese Medicine Research (Ministry of Education of China), Hunan Normal University.

Funding

This study was funded by National Natural Science Foundation of China (NSFC, Project no. 21522502), the Fundamental Research Funds for the Central Universities, the Excellent Youth Scholars of Hunan Provincial Education Department (17B155), and the Opening Fund of Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education of China), Hunan Normal University.

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  1. Yuanyuan Ma and Xiao Wang contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, College of Chemistry, Nankai University, Tianjin, 300071, China

    Yuanyuan Ma, Xiao Wang, Hua Liu & Lehui Xiao

  2. Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Key Laboratory of Phytochemical R&D of Hunan Province, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410082, Hunan, China

    Lin Wei

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  1. Yuanyuan Ma
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The manuscript was written through contributions of all the authors. All the authors have given approval to the final version of the manuscript.

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Published in the topical collection Young Investigators in (Bio-)Analytical Chemistry with guest editors Erin Baker, Kerstin Leopold, Francesco Ricci, and Wei Wang.

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Ma, Y., Wang, X., Liu, H. et al. Recent advances in optical microscopic methods for single-particle tracking in biological samples. Anal Bioanal Chem 411, 4445–4463 (2019). https://doi.org/10.1007/s00216-019-01638-z

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