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A bi-end injection capillary electrophoresis method for simultaneous determination of 37 cations and anions in beers

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Abstract

Capillary electrophoresis (CE) is excellent at separating all the ions in a sample but is rarely used as a result of its detection issue and easy loss of very fast ions by common one-end injection methods. Herein we propose a newly developed method aimed at simultaneous determination of positive and negative ions with a home-made CE device, featuring bi-end injection and contactless conductivity detection at the middle. By simply using 2.5 M acetic acid as a running buffer, the method can separate 37 ions (3 inorganic anions, 8 inorganic cations, 10 biogenic amines, and 16 amino acids) per run, with linearity between 10 and 2000 μM (R2 > 0.99), limit of detection of 1.0–16.6 μM, and limit of quantification of 2.3–31.7 μM. The recovery measured by spiking standards into samples at high, middle, and low levels was between 73% and 110%. The intra- and interday repeatability of the 37 analytes ranged from 0.69% to 8.97% and from 0.68% to 11.04%, respectively. The proposed method was evaluated by analysis of 21 beers and, in addition to acquiring the concentration information, the brands of the tested beers were distinguished. This method is of high throughput, fast, and cost-effective. It could be a promising tool for ionomic analysis.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Nos. 21727809, 21475136, 21235007 & 21621062) and Chinese Academy of Sciences (QYZDJ-SSW-SLH034).

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Authors and Affiliations

  1. Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China

    Chao Guo, Zhenpeng Guo & Yi Chen

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Chao Guo, Zhenpeng Guo & Yi Chen

  3. Beijing National Laboratory for Molecular Sciences, Beijing, 100190, China

    Yi Chen

Authors
  1. Chao Guo
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  2. Zhenpeng Guo
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  3. Yi Chen
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Correspondence to Zhenpeng Guo or Yi Chen.

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Published in the topical collection New Insights into Analytical Science in China with guest editors Lihua Zhang, Hua Cui, and Qiankun Zhuang.

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Guo, C., Guo, Z. & Chen, Y. A bi-end injection capillary electrophoresis method for simultaneous determination of 37 cations and anions in beers. Anal Bioanal Chem 411, 4113–4121 (2019). https://doi.org/10.1007/s00216-018-1507-7

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  • DOI: https://doi.org/10.1007/s00216-018-1507-7

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