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Determination of chemical purity and isotopic composition of natural and carbon-13-labeled arsenobetaine bromide standards by quantitative1H-NMR

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Abstract

In this study, we report the characterization of three arsenobetaine-certified reference materials by quantitative NMR. We have synthesized an arsenobetaine bromide high-purity standard of natural isotopic composition (ABET-1) and two carbon-13-labeled isotopic standards (BBET-1 and CBET-1). Assignments of the chemical purity and isotopic composition are not trivial in the case of arsenobetaine, and in this study we utilized quantitative1H-NMR techniques for the determination of the mass fractions (chemical purity). The isotopic purity of all three standards was also assessed by NMR from the carbon-13 satellite signals. The standards are non-hygroscopic, high-purity (ca. 0.99 g/g), and the carbon-13 enrichment for both isotopic standards is x(13C)≈0.99. These standards are designed for use as primary calibrators for mass spectrometric determination of arsenobetaine in environmental samples.

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Authors and Affiliations

  1. Measurement Science and Standards, National Research Council Canada, 1200 Montreal Road, Ottawa, ON K1A 0R6, Canada

    Phuong-Mai Le, Donald M. Leek, Zoltan Mester, Anthony Windust & Juris Meija

  2. Security and Disruptive Technologies, National Research Council Canada, 1200 Montreal Road, Ottawa, ON K1A 0R6, Canada

    Jianfu Ding

  3. Energy, Mining and Environment, National Research Council Canada, 1200 Montreal Road, Ottawa, ON K1A 0R6, Canada

    Gilles Robertson

Authors
  1. Phuong-Mai Le
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  2. Jianfu Ding
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  3. Donald M. Leek
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  4. Zoltan Mester
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  5. Gilles Robertson
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  6. Anthony Windust
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  7. Juris Meija
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Correspondence to Juris Meija.

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Le, PM., Ding, J., Leek, D.M. et al. Determination of chemical purity and isotopic composition of natural and carbon-13-labeled arsenobetaine bromide standards by quantitative1H-NMR. Anal Bioanal Chem 408, 7413–7421 (2016). https://doi.org/10.1007/s00216-016-9827-y

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  • DOI: https://doi.org/10.1007/s00216-016-9827-y

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