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Identification of lipid- and protein-based binders in paintings by direct on-plate wet chemistry and matrix-assisted laser desorption ionization mass spectrometry

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Abstract

Direct on-target plate processing of small (ca. 100 μg) fragments of paint samples for MALDI-MS identification of lipid- and protein-based binders is described. Fragments were fixed on a conventional stainless steel target plate by colloidal graphite followed by in situ fast tryptic digestion and matrix addition. The new protocol was first developed on paint replicas composed of chicken egg, collagen, and cow milk mixed with inorganic pigments and then successfully applied on historical paint samples taken from a fifteenth century Italian panel painting. The present work contributes a step forward in the simplification of binder identification in very small paint samples since no conventional solvent extraction is required, speeding up the whole sample preparation to 10 min and reducing lipid/protein loss.

Paint binder identification in a 15th-century Vivarini panel painting by direct on-plate wet chemistry followed by MALDI-MS

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Acknowledgments

This work was carried out in the framework of the PRIN 2010-11 funded by the Italian Ministry of Education, University and Research (code H91J12000130001).

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Authors and Affiliations

  1. Dipartimento di Chimica, Università degli Studi di Bari Aldo Moro, Via Orabona 4, 70125, Bari, Italy

    Cosima Damiana Calvano, Inez Dorothé van der Werf, Francesco Palmisano & Luigia Sabbatini

  2. Centro Interdipartimentale S.M.A.R.T., Università degli Studi di Bari Aldo Moro, Via Orabona 4, 70125, Bari, Italy

    Cosima Damiana Calvano & Francesco Palmisano

  3. Centro Interdipartimentale “Laboratorio di ricerca per la diagnostica dei Beni Culturali”, Università degli Studi di Bari Aldo Moro, Via Orabona 4, 70125, Bari, Italy

    Luigia Sabbatini

Authors
  1. Cosima Damiana Calvano
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  2. Inez Dorothé van der Werf
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  3. Francesco Palmisano
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  4. Luigia Sabbatini
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Corresponding author

Correspondence to Inez Dorothé van der Werf.

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Published in the topical collection celebrating ABCs 13th Anniversary.

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Calvano, C.D., van der Werf, I.D., Palmisano, F. et al. Identification of lipid- and protein-based binders in paintings by direct on-plate wet chemistry and matrix-assisted laser desorption ionization mass spectrometry. Anal Bioanal Chem 407, 1015–1022 (2015). https://doi.org/10.1007/s00216-014-8359-6

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  • DOI: https://doi.org/10.1007/s00216-014-8359-6

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