Abstract
A method for conducting fast and efficient capillary electrophoresis (CE) based on short separation capillaries in vertical alignment was developed. The strategy enables for high-throughput analysis from small sample vials (low microliter to nanoliter range). The system consists of a lab-made miniaturized autosampling unit and an amperometric end-column detection (AD) cell. The device enables a throughput of up to 200 separations per hour. CE-AD separations of a dye model system in capillaries of only 4 to 7.5 cm length with inner diameters (ID) of 10 or 15 μm were carried out under conditions of very high electric field strengths (up to 3.0 kV/cm) with high separation efficiency (half peak widths below 0.2 s) in less than 3.5 s migration time. A non-aqueous background electrolyte, consisting of 10 mM ammonium acetate and 1 M acetic acid in acetonitrile, was used. The practical suitability of the system was evaluated by applying it to the determination of dyes in overhead projector pens.
Similar content being viewed by others
References
Opekar F, Coufal P, Stulik K (2009) Chem Rev 109:4487
Vandaveer WR, Pasas SA, Martin RS, Lunte SM (2002) Electrophoresis 23:3667
Huang X, Zare RN, Sloss S, Ewing AG (1991) Anal Chem 63:189
Wallingford RA, Ewing AG (1987) Anal Chem 59:1762
Buglione L, See HH, Hauser PC (2013) Electrophoresis 34:2072
Zhong M, Lunte SM (1996) Anal Chem 68:203
Matysik FM (1998) Electrochim Acta 43:3475
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
ESM 1
(PDF 173 kb)
Rights and permissions
About this article
Cite this article
Mark, J.J.P., Piccinelli, P. & Matysik, FM. Very fast capillary electrophoresis with electrochemical detection for high-throughput analysis using short, vertically aligned capillaries. Anal Bioanal Chem 406, 6069–6073 (2014). https://doi.org/10.1007/s00216-014-7993-3
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00216-014-7993-3