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The M1 muscarinic acetylcholine receptor regulates the surface expression of the AMPA receptor subunit GluA2 via PICK1

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Abstract

Muscarinic acetylcholine receptors (mAChRs) have been shown to play significant roles in the regulation of normal cognitive processes in the hippocampus, and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs) are also involved in these processes. This study aims to explore the mAChR-mediated regulation of AMPARs GluA2 trafficking and to reveal the key proteins and the signaling cascade involved in this process. Primary hippocampal neurons, as cell models, were treated with agonist 77-LH-28-1 and antagonist VU0255035, Fsc231, and APV. C57BL/6J male mice were stereotactically injected with 77-LH-28-1 and Fsc231 to obtain hippocampal slices. The trafficking of GluA2 was detected by surface biotinylation and immunostaining. Activation of M1 mAChRs promoted endocytosis and decreased the postsynaptic localization of the AMPA receptor subunit GluA2 and that phosphorylation of GluA2 at Ser880 was increased by M1 mAChR activity. Fsc231 blocked the endocytosis and postsynaptic localization of GluA2 induced by 77-LH-28-1 without affecting the phosphorylation of Ser880. PICK1 was required for M1 mAChR-mediated GluA2 endocytosis and downstream of phosphorylation of GluA2-Ser880, and the PICK1-GluA2 interaction was essential for M1 mAChR-mediated postsynaptic expression of GluA2. Taken together, our results show a functional correlation of M1 mAChRs with GluA2 and the role of PICK1 in their interplay.

Graphical abstract

The schematic diagram for the modulation of GluA2 trafficking by M1 mAChRs. Activation of M1 mAChRs induces PKC activation, and the interaction of PICK1-GluA2 determines the endocytosis and postsynaptic localization of GluA2

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Data availability

The datasets generated and analyzed during the present study are not publicly available due to unfinished follow-up research but are available from the corresponding author upon reasonable request.

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Acknowledgements

We are very grateful to Professor Yu Qiu for providing us with 77-LH-28-1 and to Dr. Lanxue Zhao for her methodological knowledge.

Funding

This work was supported by the National Natural Science Foundation of China (81703478).

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Authors and Affiliations

  1. Department of Pharmacy, Children’s Hospital of Soochow University, 92# Street Zhongnan, Soochow, 215025, Jiangsu, China

    Zengyan Zhu, Wenjuan Wang, Chao Gu, Mei Wang & Yinghui Yan

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  1. Zengyan Zhu
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  2. Wenjuan Wang
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  3. Chao Gu
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  4. Mei Wang
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  5. Yinghui Yan
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Contributions

Y.-H. Yan designed the study and supervised the work; Z.-Y. Zhu, W.-J. Wang, and C. Gu performed the experiment; M. Wang and Y.-H. Yan analyzed the data; Z.-Y. Zhu and Y.-H. Yan wrote the manuscript; and all authors reviewed the manuscript.

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Correspondence to Yinghui Yan.

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Ethics approval

This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Ethics Committee of Children’s Hospital of Soochow University (March 2018).

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The authors declare no competing interests.

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Zhu, Z., Wang, W., Gu, C. et al. The M1 muscarinic acetylcholine receptor regulates the surface expression of the AMPA receptor subunit GluA2 via PICK1. Psychopharmacology 240, 239–248 (2023). https://doi.org/10.1007/s00213-022-06304-4

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