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Evaluation of coenzyme Q10 combined with or without N-acetyl cysteine or atorvastatin for preventing contrast-induced kidney injury in diabetic rats

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Abstract

Combined antioxidants effect for prevention of contrast-induced nephropathy (CIN) remains unclear. This study assessed the potential protective effects of coenzyme Q10 (CoQ10) alone or combined with N-acetyl cysteine (NAC) or atorvastatin against CIN in diabetic rats. Animals were randomly divided into five groups, including control and four disease groups with CIN and diabetes. Group 2 included diabetic rats with CIN. Groups 3–5 included diabetic rats that received CoQ10, CoQ10 and NAC, or CoQ10 and atorvastatin, respectively, before CIN induction. Serum, urine, and tissue were collected to evaluate renal protective effects of tested agents. Renal biomarkers, oxidative stress, and histopathological alterations were investigated. Rats with CIN showed significant renal impairment as revealed by the deleterious effects on kidney function and histology. While induction of CIN did not affect the renal levels of catalase, glutathione peroxidase (GPx), and thiobarbituric acid reactive substances, pretreatment of animals with CoQ10/NAC showed significant increase in GPx and catalase levels versus controls. Lastly, pretreatment with CoQ10/atorvastatin showed regenerative effect on distal tubules with mild kidney histology alterations relative to CIN rats. The combined use of CoQ10/atorvastatin could be a potential strategy to prevent CIN. However, future studies are warranted to test different combinations for longer prophylactic periods.

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Abbreviations

AKI:

acute kidney injury

CIN:

contrast-induced nephropathy

CoQ10:

coenzyme Q10

GPx:

glutathione peroxidase

IP:

intraperitoneal

L-NAME:

NG-nitro-L-arginine methyl ester

NAC:

N-acetyl cysteine

STZ:

streptozotocin

TBARS:

thiobarbituric acid reactive substances.

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Funding

This work was funded by a grant from the Deanship of Research, Jordan University of Science and Technology (Grant number: 380/2018).

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Authors and Affiliations

  1. Department of Clinical Pharmacy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, 22110, Jordan

    Osama Y. Alshogran, Shreen D. Nusair, Karem H. Alzoubi, Abdullah Obeidat & Maya Sweidan

  2. Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, 22110, Jordan

    Tamam El-Elimat

Authors
  1. Osama Y. Alshogran
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  2. Shreen D. Nusair
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  3. Tamam El-Elimat
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  4. Karem H. Alzoubi
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  5. Abdullah Obeidat
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  6. Maya Sweidan
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Contributions

OYA: Idea generation and conceptualization, experimental design, methodology, data processing, analysis and interpretation, manuscript writing. SDN: Experimental design, methodology, data processing and interpretation, manuscript writing. TE: Methodology, data interpretation, manuscript writing. KHA: Experimental design, data interpretation, manuscript writing. AO: Experimental work and data processing. MS: Experimental work and data processing. All authors have approved the final version of the manuscript and all data were generated in-house and that no paper mill was used.

Corresponding author

Correspondence to Osama Y. Alshogran.

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Compliance with ethical standards

The Animal Care and Use Committee of JUST approved the study protocol (protocol approval number 16/3/3/320). The study was conducted in accordance with the standards of Guide for the US National research Council’s and Laboratory.

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The authors declare that they have no conflicts of interest.

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Alshogran, O.Y., Nusair, S.D., El-Elimat, T. et al. Evaluation of coenzyme Q10 combined with or without N-acetyl cysteine or atorvastatin for preventing contrast-induced kidney injury in diabetic rats. Naunyn-Schmiedeberg's Arch Pharmacol 394, 1403–1410 (2021). https://doi.org/10.1007/s00210-021-02070-w

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