Abstract
Glucose, which plays an essential role in carbon and energy metabolism in eukaryotes, is vital in directing various energy-consuming cellular processes. In S. cerevisiae, transcription factors involved in regulating hexose transporters and their mechanisms of action under different carbon sources were revealed in detail. However, there is limited information on these processes in S. pombe. In this study, the effect of SPCC320.03 (named SpRgt1), the ortholog of ScRgt1 whose molecular mechanism is known in detail in S. cerevisiae, on the transcriptional regulation of hexose transporters (ght1-8) dependent on different carbon sources was investigated. We measured the transcript levels of ght1-8 using the qPCR technique and performed relative evaluation in S. pombe strains (parental, rgt1 deleted mutant, rgt1 overexpressed, and vectoral rgt1 carrying mutant). We aimed to investigate the transcriptional changes caused by the protein product of the rgt1 (SPCC320.03) gene in terms of ght1-8 genes in strains that are grown in different carbon sources (2% glucose, 2% glycerol + 0.1% glucose, and 2% gluconate). Here, we show that SpRgt1 is involved in the regulation of the ght3, ght4, ght6, and ght7 genes but that the ght1, ght2, ght5, and ght8 gene expression vary depending on carbon sources, independently of SpRgt1.
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This study was funded by Scientific Research Projects Coordination Unit of Istanbul University. Project number: 34756.
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by MSI, MT, MY, SY, BP. The first draft of the manuscript was written by MSI, MT, MY, SY, BP, SKU, ATS. All authors have commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Communicated by Yusuf Akhter.
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Ibisoglu, M.S., Tan, M., Yilmazer, M. et al. Effects of ScRgt1-Like DNA-binding transcription factor SpRgt1 (SPCC320.03) on Hexose transporters gene expression in Schizosaccharomyces pombe. Arch Microbiol 206, 155 (2024). https://doi.org/10.1007/s00203-024-03901-z
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DOI: https://doi.org/10.1007/s00203-024-03901-z