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Unravelling the transcriptome response of Enterobacter sp. S-33 under varying temperature

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Abstract

Enterobacter genus includes the bacteria occupying every aspect of environment, however, their adaptability at varying temperature is not clear. In the present study, we analyzed the transcriptome response of Enterobacter sp. S-33 and their functional genes under various temperatures (30–45 ℃) that were expressed and accumulated in cells under temperature-stress. During a temperature shift from 37 to 45 ℃, 165 genes showed differential expression including 112 up-regulated and 53 down-regulated. In particular, heat-shock genes such as CspA, 16 kDa heat shock protein A/B and transcriptional regulators such as LysR, TetR, and LuxR were differentially expressed, indicating the role of complex molecular mechanism of Enterobacter adapting to temperature stress. Similarly, genes associated to signal transduction, ABC transporters, iron homeostasis, and quorum sensing were also induced. The Gene ontology enrichment analysis of differentially expressed genes (DEGs) were categorized into “transmembrane transport”, “tRNA binding”, “hydrogen sulfide biosynthetic process” and “sulfate assimilation” terms. In addition, Kyoto Encyclopedia of Genes and Genomes pathways showed that ABC transporter as well as quorum sensing pathways were significantly enriched. Overall, current study has contributed to explore the adaptive molecular mechanisms of Enterobacter spp. upon temperature change, which further opens new avenues for future in-depth functional studies.

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Acknowledgements

RPS acknowledges the Department of Biotechnology, Government of India for providing the Ramalingaswami Re-entry Fellowship.

Funding

The work was supported by the Ramalingaswami Re-entry Grant provided by Government of India.

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Authors and Affiliations

  1. Department of Bioengineering and Biotechnology, Birla Institute of Technology, Mesra, Ranchi, Jharkhand, 835215, India

    Kiran Kumari

  2. Department of Plant Sciences and Landscape Architecture, University of Maryland, College Park, MD, 20742, USA

    Parva Kumar Sharma

  3. Department of Biotechnology, Jaypee Institute of Information Technology, Noida, India

    Rajnish Prakash Singh

Authors
  1. Kiran Kumari
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  2. Parva Kumar Sharma
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  3. Rajnish Prakash Singh
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Contributions

KK analysed the data. PKS did the gene network analysis. RPS wrote the manuscript and supervised the work.

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Correspondence to Rajnish Prakash Singh.

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Communicated by Yusuf Akhter.

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Kumari, K., Sharma, P.K. & Singh, R.P. Unravelling the transcriptome response of Enterobacter sp. S-33 under varying temperature. Arch Microbiol 206, 81 (2024). https://doi.org/10.1007/s00203-023-03792-6

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  • DOI: https://doi.org/10.1007/s00203-023-03792-6

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